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Optimization Of The Expression Of Surface Antigen Sag1/2 Of Toxoplasma Gondii In The Yeast Pichia Pastoris, Init Ithoi
Optimization Of The Expression Of Surface Antigen Sag1/2 Of Toxoplasma Gondii In The Yeast Pichia Pastoris, Init Ithoi
Init Ithoi
Surface antigens are the most abundant proteins found on the surface of the parasite Toxoplasma gondii. Surface antigen 1 (SAG1) and Surface antigen 2 (SAG2) remain the most important and extensively studied surface proteins. These antigens have been identified to play a role in host cell invasion, immune modulation, virulence attenuation. Recombinant SAG1/2 was cloned and expressed in yeast Pichia pastoris. We describe here optimization of critical parameters involved in high yield expression of the recombinant SAG1/2. Our results suggest that recombinant SAG1/2 were best expressed at 30 masculineC, pH 6 and 1% methanol as the carbon source by X33 …
Cloning And Expression Of Toxoplasma Gondii Dense Granular Protein 4 (Gra4) In Pichia Pastoris, Init Ithoi
Cloning And Expression Of Toxoplasma Gondii Dense Granular Protein 4 (Gra4) In Pichia Pastoris, Init Ithoi
Init Ithoi
GRA4 of Toxoplasma gondii has been shown to prompt IgG, IgM and IgA responses in previous studies and is thus considered one of the major immunogenic proteins from T. gondii that can be used for both diagnostics purposes and vaccine development. This study seeks to clone and express the GRA4 in Pichia pastoris, which has numerous advantages over other systems for expression of eukaryotic proteins. In order to achieve this, the gene was cloned into the pPICZ alpha A expression vector, which was then incorporated into the P. pastoris genome via insertional integration for expression of the recombinant protein, under …