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SelectedWorks

Dentistry

2005

Articles 1 - 9 of 9

Full-Text Articles in Medicine and Health Sciences

Human Telomerase Reverse Transcriptase Expression In Oral Carcinogenesis - A Preliminary Report, Kumar S. K. S., Rosnah Binti Zain, Ismail S. M., Cheong S. C. Dec 2005

Human Telomerase Reverse Transcriptase Expression In Oral Carcinogenesis - A Preliminary Report, Kumar S. K. S., Rosnah Binti Zain, Ismail S. M., Cheong S. C.

Prof. Dr. Rosnah Binti Zain

Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, is strongly associated with telomerase activity implicated in cellular immortalization and tumorigenesis. In situ detection of hTERT will aid in determining the localization of telomerase positive cells. The aim of this study was to detect hTERT protein expression in multistep oral carcinogenesis using paraffin embedded tissue samples, and to study the relationship of hTERT expression with different histological stages in oral carcinogenesis. Normal (n=4), hyperplastic (n=4), dysplastic (n=4) and neoplastic (n=10) oral epithelia representing different histological stages in oral carcinogenesis were included in the study. hTERT protein detection was done …

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Proliferative And Apoptotic Activity At The Tumour Invasive Front In Oral Squamous Cell Carcinoma, W.C. Ngeow, R. B. Zain, Z. Abidin, K. S. Phaik, S. Kumar Apr 2005

Proliferative And Apoptotic Activity At The Tumour Invasive Front In Oral Squamous Cell Carcinoma, W.C. Ngeow, R. B. Zain, Z. Abidin, K. S. Phaik, S. Kumar

Wei Cheong Ngeow

Introduction: the tumour invasive front of squamous cell carcinoma (OSCC) has long been recognized as an important area which harbour prognostic information. The aim of the present study was to describe the expression of K1-67, MDM2 and Bcl-2 at the tumour invasive front in the buccal mucosa and also to investigate whether there is any association between the expression of K1-67, MDM2 and Bcl-2 with the certain sociodemographic characteristics, clinical and histopathological parameters. Materials and Methods: The sample of this study consisted of 31 biopsy cases of OSCC from the buccal mucosa. Immunohistochemistry was used with specific antibodies to K1-67, …

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Oral Cancer And Precancer Research In Malaysia - The Database And Tissue Resource Bank, Rosnah Binti Zain Apr 2005

Oral Cancer And Precancer Research In Malaysia - The Database And Tissue Resource Bank, Rosnah Binti Zain

Prof. Dr. Rosnah Binti Zain

Introduction: Uncoordinated data collection by different research groups prompted the initiation of Malaysian oral cancer Research Initiative (MOCRI) group. Objectives: To coordinate and standardize data and tissue collection and storage, develop a minimum dataset on risk factors, intervention techniques and quality of life of oral cancer patients. Materials and Methods: A computer software programme is currently being developed to accommodate multi-centre data collection and research activities in eight hospitals. The complete dataset includes parameters on sociodemographic, clinical, pathological, quality of life measures, details of treatment methods, vital status and dietary intake. Tissues are being collected, stored and catalogued as fresh …

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Immunohistochemical Localization Of Htert Protein In Oral Carcinogenesis, Kumar S., Ismail S. M., Rosnah Binti Zain, Abraham M. T., Cheong S. C. Apr 2005

Immunohistochemical Localization Of Htert Protein In Oral Carcinogenesis, Kumar S., Ismail S. M., Rosnah Binti Zain, Abraham M. T., Cheong S. C.

Prof. Dr. Rosnah Binti Zain

Introduction: Human telomerase reverse transcriptase (hTERT) the catalytic subunit of telomerase is associated with cellular immortalization and tumorigenesis .In situ detection of hTERT using immunohistochemistry (IHC) allows localizing the hTERT- positive cells and also overcomes the limitation of requirement of fresh tissues for molecular techniques such as Telomeric Repeat Amplification Protocol (TRAP) for telomerase/ hTERT detection. The objectives of this study are to detect hTERT protein expression using immunohistochemistry in different histological stages in oral carcinogenesis with paraffin embedded tissue samples and cultured cells and to relate the hTERT expression with different histological stages in oral carcinogenesis. Materials and Methods: …

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Expression Of P53, Prb, Ki-67 And Bcl-2 In Betel Quid Induced Oral Mucosal Lesions, Kipli N. P., Kumar S. K. S., Rosnah Binti Zain, Fan C. Y., Boon P. K. Apr 2005

Expression Of P53, Prb, Ki-67 And Bcl-2 In Betel Quid Induced Oral Mucosal Lesions, Kipli N. P., Kumar S. K. S., Rosnah Binti Zain, Fan C. Y., Boon P. K.

Prof. Dr. Rosnah Binti Zain

Introduction: Alterations in p53, pRb, KI-67 and bcl-2 have been implicated the development of oral cancer although their combined expression in betel quid induced oral mucosal lesions as not clear. The objective of this study is to investigate the expressions of p53, pRb, K1-67 and bcl-2 m oral mucosa of betel quid chewing subjects. Materials and Methods: A total of 64 tissue sections were obtained from biopsy samples of 50 subjects clinically diagnosed with betel chewer's mucosa (BCM, n=32), BCM with leukoplakia (n=12) and leukoplakia (n=6). Oral squamous cell carcinoma (OSCC, 11=10) and normal oral mucosa (n=4) section were used …

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Clinical Pathological Evaluation And Risk Factors Of Oral Cancer Cases Of East Coast Of Peninsular Malaysia, Farini M. S., Azlina A., Rushdan I., Manoharan M., Rosnah Binti Zain, Samsudin A. R. Apr 2005

Clinical Pathological Evaluation And Risk Factors Of Oral Cancer Cases Of East Coast Of Peninsular Malaysia, Farini M. S., Azlina A., Rushdan I., Manoharan M., Rosnah Binti Zain, Samsudin A. R.

Prof. Dr. Rosnah Binti Zain

Introduction: Oral cancer is one of the common cancers in Malaysia. Tile population of east coast of Peninsular Malaysia has a different hfestyle and Malay is the predominant race. Oral cancer research in this area started since the Malaysian National Oral Cancer group was established. The aim: of this study is to evaluate the clinical pathological findings and to investigate the role of tobacco smoking, alcohol consurnption and betel quid chewing as tile risk factors among oral cancer cases in east coast of peninsular Malaysia. Materials and Methods: Fifty case records of only treated/operated cases of patients with oral cancer …

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Establishment And Characterization Of An Oral Squamous Carcinoma Cell Line From A Patient With No Apparent Habits Associated With Oral Cancer, Sok C. C., Hamid S., Peng L. K., Ismail S. M., Rosnah Binti Zain Jan 2005

Establishment And Characterization Of An Oral Squamous Carcinoma Cell Line From A Patient With No Apparent Habits Associated With Oral Cancer, Sok C. C., Hamid S., Peng L. K., Ismail S. M., Rosnah Binti Zain

Prof. Dr. Rosnah Binti Zain

Introduction: We have established a cell line ORL-48(T) from a surgically resected specimen of an untreated primary human oral squamous cell carcinona of the mandible.This patient did not appear to have any oral habits which have been reported to be associated with oral cancer Materials and Method: The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, HPV infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of the ORL-48(T) cell line were investigated. Results: The ORL-48(T) cell line is immortal, 3T3- independent and grew as a monolayer with the doubling time of …

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Darier Disease: A Case Report, Jalil A. A., Rosnah Binti Zain, Van Der Waal, I. Jan 2005

Darier Disease: A Case Report, Jalil A. A., Rosnah Binti Zain, Van Der Waal, I.

Prof. Dr. Rosnah Binti Zain

Darier disease, also known as keratosis follicularis, dyskeratosis follicularis, and benign dyskeratosis, is a rare disorder of keratinisation that primarily affects the skin and, to a lesser extent, the oral mucosa. It was described independently by both Darier and White in 1889. It has a prevalence of 1:100,000 of the population and is inherited as an autosomal dominant trait.1 The age of onset is childhood or adolescence. Patients usually present with multiple small firm reddish-brown papules on the forehead, scalp, neck, shoulders, chest, and limbs. Other cutaneous signs include punctate keratotic pits of the palms and soles and dystrophy of …

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The Effects Of Oxygen-Based Disinfectant On Dimensional Accuracy Of Alginates, Noor Hayaty Abu Kasim Abu Kasim N.H. Jan 2005

The Effects Of Oxygen-Based Disinfectant On Dimensional Accuracy Of Alginates, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objective: This study evaluated the effect of an active oxygen based disinfectant, 2% Perform® (Schulke & Mayr, Germany) on the dimensional accuracy of four alginates, Alginoplast (Heraeus Kulzer, Holland), Kromopan (LASCOD, Italy), Alginmax (MAJOR, Italy) and Duplast (Dentsply, China). Method: A cobalt chromium master model with four cylindrical studs representing 2 canines and 2 molars was constructed. A total of 40 impressions for each alginates were taken and treated in 4 different conditions, Immediate pour (control); 10 minutes immersion in distilled water; 10 minutes immersion in 2% Perform® and 30 minutes in 2% Perform®. The impressions were then poured in …

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