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Composition And Functional Potential Of The Human Mammary Microbiota Prior To And Following Breast Tumor Diagnosis, Courtney Hoskinson, Kelly Zheng, Jaelyn Gabel, Annie Kump, Rana German, Ram Podicheti, Natascia Marino, Leah T. Stiemsma

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Microbiota studies have reported changes in the microbial composition of the breast upon cancer development. However, results are inconsistent and limited to the later phases of cancer development (after diagnosis). We analyzed and compared the resident bacterial taxa of histologically normal breast tissue (healthy, H, n = 49) with those of tissues donated prior to (prediagnostic, PD, n = 15) and after (adjacent normal, AN, n = 49, and tumor, T, n = 46) breast cancer diagnosis (n total = 159). DNA was isolated from tissue samples and submitted for Illumina MiSeq paired-end sequencing of the V3-V4 region of the …