Medicine and Health Sciences Commons^™

Regulation Of Hyaluronate Lyase (Hyla) Expression By Regr A Transcriptional Repressor In Streptococcus Pyogenes, Alexis A. Kordis

Biological Sciences Theses & Dissertations

Streptococcus pyogenes, group A streptococci (GAS), causes diseases ranging from asymptomatic to life threatening. Some strains of S. pyogenes produce extracellular hyaluronate lyase (HylA), a potential virulence factor. HylA is an enzyme that degrades hyaluronic acid, which is found in the extracellular matrix of human tissues. The breakdown of the host tissue contributes to the spread of infection. The hylA gene is not constitutively expressed in vitro, which implies regulation.

A proposed regulator of hyaluronate lyase expression is RegR, a LacI/GalR like protein. A vector containing disrupted regR was electro-transformed into S. pyogenes M-type 22 strain 10403. Growth characteristics …

Use Of Immersive Visualization For The Control Of Dental Anxiety During Dental Hygiene Treatment, Carmelo Padrino-Barrios

Dental Hygiene Theses & Dissertations

Purpose: The purpose of this study was to determine the effectiveness of immersive visualization (IV) therapy, a technique that provides visual and stereoscopic display through the use of eyewear, in anxious patients during an oral prophylaxis. Methods: A convenience sample of thirty adults was enrolled. A split mouth design was utilized. Subjects were randomly divided into two groups: group A (use of IV for the right side of the mouth), and group B (use of IV for the left side of the mouth). Subjects received a full mouth oral prophylaxis (removal of supra and subgingival calculus and selective polishing), always …

Child Emotional Abuse And Its Relationship To Obesity, Kelly Anderson Driscoll

Sociology & Criminal Justice Theses & Dissertations

Over the past 30 years in the United States childhood obesity has more than tripled (Center for Disease Control and Prevention, 2010). In 2008, 18.5% of adolescents of the age 12-18 years old were obese in the United States (Center for Disease Control and Prevention, 2010). With the increase of obesity Americans have spent approximately 9% of their total medical costs on obesity-related illnesses (Finkelstein, Fiebelkorn, &Wang, 2003). Previous research documented the relationship between virtually every form of child abuse and subsequent obesity. Restricting focus to child emotional abuse, while studies have linked emotional abuse to the long-term consequence of …

Experiences Of Resident Assistants With Potentially Suicidal Students: Identification, Referral, And Expectations, Katherine M. Bender

Counseling & Human Services Theses & Dissertations

Resident Assistants (RAs), living on campus and tasked with advising students while keeping them safe, are in a position to identify and refer students who may be at risk for suicide or other mental health issues. This study examined RA ability to identify students at risk for suicide, RA comfort in working with students at risk for suicide, RA actions taken when working with students who may be at risk for suicide, and RA expectations for shared information about students the RAs have referred for counseling because they may be at risk for suicide. The study found that RAs report …

Student Retention In Bsn Programs, Katherine Pittman Hensley

Educational Foundations & Leadership Theses & Dissertations

This study examined, by use of a researcher-developed survey instrument, perceptions between three groups on reasons why students drop out of nursing programs. Also examined are recommendations from the three groups on how to try to avoid nursing student attrition. Specific groups surveyed included native BSN students, RNB students, and a mixed group of nursing faculty. Survey items were divided into two general groups: student-related issues, and institutional issues. Since RNB students (which include a larger number of non-traditional students) and BSN students (which include more of the native university population of traditional students) have many differences, the author attempted …

In Vitro Selection Of Chloroquine Tolerant Plasmodium Falciparum Parasites, Daniel A. Daley

Biological Sciences Theses & Dissertations

Malaria is among the most devastating human diseases, and a majority of lethal cases are caused by the protozoan parasite, Plasmodiumfalciparum. The emergence of multi-drug resistant P.falciparum is a major obstacle to malaria control and is highlighted by the abandonment of chloroquine (CQ) as a first-line treatment of P.falciparum infections worldwide. Chloroquine resistance (CQR) is associated primarily with mutations in the transmembrane digestive vacuole protein, PfCRT. However, CQR P.falciparum parasites harboring the same mutant pfcrt allele vary in their CQ response, suggesting the CQ response is multigenic in nature. No gene outside of pfcrt is completely associated with CQR. Thus, …

Fluorescent Technology Versus Visual And Tactile Examination In The Detection Of Oral Lesion: A Pilot Study, Hadeel Mohammed Ayoub

Dental Hygiene Theses & Dissertations

Purpose: The purpose of the study was to compare the effectiveness of the VELscope® Vx, versus a visual and tactile intraoral examination in detecting oral lesions in an adult, high risk population. Methods: A convenience sample of 30 participants (17 cigarette smokers and 13 dual addiction smokers) was enrolled. For the purpose of this study, dual addition was defined as cigarettes plus hookah usage. Two trained and calibrated dental hygienists conducted all examinations. Visual and tactile intraoral examinations were conducted, followed by VELscope® Vx florescence examinations. All subjects received an inspection of the lips, labial mucosa, buccal mucosa, floor of …

Comparing Velscope Vx And Traditional Oral Exams In Shisha (Hookah) Smokers: A Pilot Study, Amanda E. Kimball

Dental Hygiene Theses & Dissertations

The purpose of this study was to compare oral findings using two exam types, the VELscope Vx® screening device versus a modified oral examination in detecting oral potentially malignant lesions in shisha smokers.

Methods: Thirty-one participants who reported currently smoking shisha and tobacco were recruited. Participants were identified for smoking habits to include two groups, to include those who reported smoking shisha exclusively and those who reported smoking shisha and tobacco. Each group received both exam types; examiners used a standardized protocol. A health history questionnaire was also collected to assess participant's oral cancer risk factors such as age, race, …

Investigating Similarities And Differences As Measured By The Durel And Gsq Between Three Subgroups Attending A Local Aa Meeting To Develop A Profile Of Long Term Attendees, Keesha Masean Kerns

Counseling & Human Services Theses & Dissertations

Alcoholics Anonymous groups are growing in popularity due to their cost effectiveness and history of dependability. Although the program clearly has the numbers to support its popularity, skeptics continually analyze its claims of effectiveness through research. While research pertaining to AA is diverse, very little is presented concerning factors that contribute to retention in the program. The primary purpose for this quantitative study was to investigate the similarities and differences in the various stages of participants of a sample attending a local AA program, identify variables that contribute to retention in the AA sample, and to use Grounded Theory to …

Changes In The Dynamics Of Postural And Locomotor Control As A Result Of Varying Task Demands, Kathleen Sherry Thomas

Human Movement Sciences & Special Education Theses & Dissertations

The aim of this study was to examine changes in postural and locomotor control under varying task demands. Three experiments were designed to address the impact that fast walking had on standing posture over time, slow walking had on gait dynamics over time, and the extent to which gait speed interacts with the ability to walk randomly.

For experiment I, the aim was to identify the time course in which postural adaptation occurred while walking at faster than preferred speeds. Postural motion was assessed at specific intervals over a 35-min walking trial. Findings revealed that walking at a faster speed …

Effects Of An Interprofessional Simulation Activity To Improve Students' Perceptions Of Other Healthcare Professions, Chase Raymond Poulsen

STEMPS Theses & Dissertations

Healthcare professionals have historically been educated and trained by members of their own profession within a curriculum that reinforces their individual discipline-specific strengths. This differentiation has contributed to students having little interaction with other professionals until after they have entered the workforce and consequently little formal education in collaboration or integration. The purpose of this mixed-method study was to evaluate the impact of an interprofessional (IP) collaborative activity on student's perceptions of the others discipline for the improvement in care of medical patients.

The sample population consisted of students from two programs, nursing (n=40) and respiratory therapy (n=33). Students were …

Patterns Of Gene Expression From Human Costal Cartilage In Relation To The Chest Wall Deformity Pectus Carinatum, Janna E. Grubbs

Biological Sciences Theses & Dissertations

Cartilage deformities within the human chest wall, specifically pectus excavatum (PE) and pectus carinatum (PC) are common (1/400-1/1000) and yet, despite their clinical significance, are some of the least studied disorders pertaining to cartilage [1]. The costal cartilage connecting "false ribs" 8-10 to the sternum is often abnormally grown and can lead to formation of a severely sunken "funnel" chest (PE) or push outwards to form a "pigeon" chest (PC). Both conditions can have impact on the diaphragm, heart, lungs, and psychological function. An established ratio of PE and PC in males to females is 4:1, indicating a sex-linked male …

Protective Behavioral Strategy Subtypes As Moderators Of The Relationship Between Alcohol Consumption And Alcohol-Related Problems, Benjamin A. Kite

Psychology Theses & Dissertations

Protective behavioral strategy (or drinking control strategy) use is widely regarded as an effective tool for reducing negative consequences from consuming alcohol (Martens et al., 2005; Martens et al., 2008). Research has shown that frequent protective behavioral strategy use buffers the relationship between alcohol consumption and alcohol-related problems (Borden et al., 2011), and that gender moderates this effect (Benton et al., 2004); however. The present research was used to expand on previous research showing that protective behavioral strategy use can buffer the relationship between alcohol consumption and alcohol-related problems. Further, the assessment of protective behavioral strategy use across gender was …

Novel Architecture Of Costal Cartilage And Implications In Chest Wall Deformities, Anthony J. Asmar

Biological Sciences Theses & Dissertations

Costal cartilage is a type of hyaline cartilage that forms rod-like structures that connect the ribs to the sternum. Deformation of costal cartilage is observed in the chest wall deformities, pectus excavatum and pectus carinatum. Pectus excavatum involves a sternal displacement causing a depression of the chest while pectus carinatum causes a protrusion of the chest. As costal cartilage is not a widely studied tissue, this leaves little knowledge into possible factors involved in the pathogenesis of pectus deformities. Costal cartilage in these deformities has been described as being weakened and may implicate proteoglycans which play an important role in …

Functional Near Infrared Detection Of Real And Imagined Finger Taps Using Support Vector Machine, Linear Discriminant Analysis, And Decision Tree Classification Methods, Eugene A. Stoudenmire

Computational Modeling & Simulation Engineering Theses & Dissertations

This study investigates the thesis that given cerebral response samples of an individual's left, right, both, and imagined finger tapping, continuous wave (CW) functional Near Infrared (fNIR), unregistered with fMRI, can differentiate between any two of the four categories.

Fifty subjects were outfitted with a single source/detector attached to a single, square pad, affixed to their heads using devices such as elastic bands and caps for light shielding. Slides depicting arrows pointing left, right, both directions, or made of dashed lines were presented to each subject, with a slide of text interspersed between each. Subjects tapped with their left finger, …

Shared Leadership In Six Sigma Teams From The North Shore-Lij Health System, Brian J. Galli

Engineering Management & Systems Engineering Theses & Dissertations

The main goal of the research was to identify the relationships that the internal research team environment and external coaching variables had with the degree of shared leadership in the context of Six Sigma teams from the North Shore LIJ Health System. Furthermore, the research ascertained the relationships that these variables had with two performance metrics: a team's ability to complete project deliverables and satisfy customer's requirements. This research also sought to understand additional factors that affected the five variables.

The research found that while shared leadership did not positively change as a team progressed through the phases of the …

Attachment Ability And Melanoma Inhibitory Activity Mrna Expression Level Changes In Murine B16-F10 Melanoma Cells Post Nanosecond Electric Pulses, Hongxia Jia

Theses and Dissertations in Biomedical Sciences

The effects of high-voltage nanosecond electric pulses (nsEPs) on metastatic melanoma are still unclear. Hence, we applied one, two, three, and four 300 ns 40 kV/cm pulses to murine B16-F10 melanoma cells. Cell attachment ability was determined by comparing the number of floating cells and the percentage of attached cells. Melanoma inhibitory activity (MIA) is a secretory protein that is highly correlated with the malignancy and metastasis of malignant melanomas. We used MIA as our target to evaluate the effect of nsEPs on metastasis. Pulsed (experimental) and unpulsed (control) cells were incubated at 37°C under a 5% CO₂ atmosphere. …