Medicine and Health Sciences Commons^™

Negative Cooperatively In The Binding Of 3-Aminopyridine Adenine Dinucleotide To L-Α-Glycero-3-Phosphate Dehydrogenase, David Wheeler

Chemistry & Biochemistry Theses & Dissertations

Glycerol-3-phosphate dehydrogenase catalyzes the reversible reaction:

NAD⁺ + G-3-P ⇌ NADH + DHAP + H⁺

The enzyme is found in organisms from E. coli to man. The primary function of the enzyme varies from organism to organism. Depending on the metabolism of the organism, the enzyme may function primarily to generate G-3-P for lipid (in organisms with a highly aerobic biosynthesis metabolism), or it may function primarily to produce NAD⁺ to support glycolysis. Complex multicellular organisms possess tissue specific isozymes of GPDH whose kinetic parameters reflect the relative importance of G-3-P and NAD⁺ production in various …

A Survey Of Burnout Among Dental Hygiene Educators, Sandra Boggs Ricks

Dental Hygiene Theses & Dissertations

A survey was conducted on 159 full-time dental hygiene educators in order to collect data on job satisfaction, morale, and perceived institutional/program effectiveness. Educators were chosen for the study by cluster sampling from two- and four-year accredited dental hygiene programs in the United States. The programs were selected at random from stratified groups according to type of institutional setting. Participants were asked to complete a questionnaire which contained four major sections: demographic data, job satisfaction, institutional/program effectiveness, and feelings related to their position. Findings were analyzed utilizing contingency tables and Chi-Square analysis. No attempt was made to establish a cause …

Two-Dimensional Electrophoretic Studies Of Normal Spematozoal Proteins, Ying Lin

Chemistry & Biochemistry Theses & Dissertations

Protein profiles of human spermatozoa have been mapped by using high resolution two-dimensional electrophoresis which combines isoelectric focusing as the first dimension and polyacrylamide gradient gels electrophoresis in SDS as the second dimension. The profiles of normal spermatozoa! extraction, acrosomal extraction and seminal plasma showed high resolution in its separation capability. No qualitative or quantitative differences were observed by using Coomassie Brilliant Blue R-250 staining in a much higher concentration of proteins. However, coomassie blue lacks the sensitivity to detect many minor components. Therefore, a silver staining procedure was used, which resulted in greatly increased sensitivity.

Samples from normal fertile …

The Effects Of Tri-O-Cresyl Phosphate On 2',3'-Cyclicnucleotide-3'-Phosphohydrolase Activity In Neural And Non-Neural Tissue Of The Rat, Mark K. Schlager

Chemistry & Biochemistry Theses & Dissertations

A total of 44 Long-Evans rats received 5.7 g/kg tri-O-cresyl phosphate (TOCP) orally every 3 days for 6 days. A second group of 13 rats received 4.6 g/kg orally every 3 days for 6 days. Animals were administered atropine sulfate (10 mg/kg) intraperitoneally every 6 hours or as needed to alleviate cholinergic symptoms. Ten control animals were given corn oil orally. Eight rats (18%) survived the 5.7 g/kg dose regimen and 5 rats (38%) survived the 4.6 g/kg dose regimen. Surviving rats were killed at 30-33 days post-dose. Signs of delayed neuropathy included ataxia and motor impairment.

The CNP activity …

Orthodontists' Attitudes Toward Expanding Career Opportunities For The Dental Hygienist In Orthodontics, Julie A. Foster

Dental Hygiene Theses & Dissertations

The purpose of this survey was to determine attitudes of orthodontists toward employing dental hygienists. A self-designed, 33 item questionnaire was used to obtain descriptive data. The questionnaire was mailed to a random sample of orthodontists chosen from the United States portion of the Orthodontic Directory of the World (N=500). Data were obtained from 298 orthodontists for a 58.6 percent response rate.

The study suggests that although orthodontists believe dental hygienists are important oral health professionals (96.2 percent) and important parts of the orthodontic practice (32.6 percent), only 10.9 percent of orthodontists surveyed employ dental hygienists. Factors determined to be …

Human Need Fulfillment Alteration In The Client With Uterine Cancer: The Registered Nurses' Perception Versus The Clients' Perception, Linda Lane Lilley

Nursing Theses & Dissertations

This study was a comparison of the similarities and differences between the registered nurses' perception and clients' perception of human need fulfillment alterations in the client with uterine cancer. The effect of level of educational preparation and number of years work experience on the nurses' perception was also analyzed. The participants were 15 registered nurses and 15 clients who had uterine cancer and were admitted to two acute care hospitals in Tidewater. Registered nurses were asked to complete the tool entitled Human Needs Assessment Scale and rate the 35 human needs in relation to the perceived importance for the client. …

A Study Of Loneliness And Health Related Complaints In Residents Of High Rise Apartment Buildings For The Elderly, Susan H. Jones

Nursing Theses & Dissertations

The purpose of this study was to explore feelings of loneliness among relocated elderly residents of age-segregated, high-rise apartment buildings and any relationship between these subjective feelings of loneliness and certain variables which were hypothesized as predictors of feelings of loneliness. The variables studied in relation to loneliness were cathectic investment, activity, and perceptions of health. A non-experimental survey design where data were collected by interviewing subjects was used. The sample consisted of 18 female residents of a high-rise apartment building for the elderly. Each was interviewed using the Loneliness Rating Scale developed by Dr. Gloria M. Francis and a …

How Registered Nurses In Skilled Nursing Facilities Rate The Importance Of And The Frequency Engaged In Specific Nursing Behaviors Related To Their Role, Christine Anne Heine

Nursing Theses & Dissertations

Registered nurses in skilled nursing facilities were surveyed using the Six-Dimension Scale of Nursing Performance to determine: (1) how they rated the importance of specific nursing behaviors related to their role, (2) how they rated the frequency engaged in the same specific nursing behaviors related to their role, and (3) if there was a relationship between how they rated the specific nursing behaviors for importance and frequency. There were five subgroups of nursing behaviors: (a) leadership, (b) nursing process, (c) teaching and collaboration, (d) interpersonal relations and communication, and (e) professional development. Registered nurses rated all of the behaviors as …

Predictors Of Appointment-Keeping Behaviors Among Users Of Adult Medical Clinics, Ruth L. Campau

Nursing Theses & Dissertations

In a descriptive correlational study, three predictors of appointment keeping behaviors of adults who attend general medical clinics in a primary health care setting were explored. The three selected predictors from the Health Belief Model, a preventive health behavior model, were: (a) perceived barriers to health care, (b) health locus of control (HLC), and (c) satisfaction with health care. The purpose of this study was to identify the predictive value of multiple variables for a specific health-related behavior. Data on the study were collected on a non-probability, purposive sample of 52 users of the adult general medical clinics. The tool …

Trait Anxiety In Female Heads Of Household, Bonnie Martinette Wheeler

Nursing Theses & Dissertations

The purpose of this investigation was to determine if being the female head of a single-parent household was associated with increased trait anxiety. Ninety-four female nurses completed the Trait portion of the State-Trait Anxiety Inventory developed by Spielberger, Gorsuch, and Lushene (1964) and a demographic inventory. The demographic inventory provided the information regarding marital status and presence or absence of children needed to delineate the sample into four groups: Group I (n=31) married females with dependent children in the home; Group II (n=10) married females with no dependent children in the home; Group III (n=26) single, separated or divorced females …

A Method For The Comparison Of Hdl Containing Apoprotein E And Hdl Cholesterol, Steven A. Knizner

Chemistry & Biochemistry Theses & Dissertations

Human serum high density lipoproteins (HDL) were isolated by preparative salt density gradient ultracentrifugation and analyzed further by heparin-sepharose affinity chromatography. Separation of the major classes of serum lipoproteins was achieved after a single ultracentrifugation on a salt density gradient at 272,000 x g for 36 hours in a swinging bucket rotor. After isolation, the total HDL was subdivided into HDL containing apoprotein E and HDL without this apoprotein by heparin-sepharose affinity chromatography. Relative amounts of these subclasses were then calculated.

HDL-cholesterol was measured after precipitation of LDL and VLDL by phosphotungstic acid and magnesium. The statistical relationship between HDL …

Investigation Of The Coenzyme Binding Site Of Lactate Dehydrogenase From Chicken Muscle, Jia-Hwei Lin

Chemistry & Biochemistry Theses & Dissertations

Investigation of the coenzyme binding site of lactate dehydrogenase was approached by using coenzyme analogs. Inhibition studies of this enzyme using n-alkylphosphates as coenzyme analogs were performed with a fluorometric method. The results showed that all of the n-alkylphosphates were competitive inhibitors with respect to NAD⁺.

The n-alkyl-ADPs were synthesized from AMP and dicycloheaylammonium salts of n-alkylphosphates. The synthetic compounds were subjected to NMR spectroscopic studies, thin layer chromatography and determination of the ratio of adenosine moiety to phosphate groups. The results indicated that the synthetic compounds were n-alkyl-ADPs.

These synthetic compounds were used as coenzyme analogs in …

Admissions Criteria As Predictors Of Students' Successful Completion Of A Dental Hygiene Program, M. Linda Brown

Dental Hygiene Theses & Dissertations

The purpose of this study was to investigate the criteria used to select dental hygiene students and to determine the ability of these criteria to predict students' successful completion of a dental hygiene program. One hundred sixteen graduates of the certificate level dental hygiene program at Old Dominion University between 1971- 1981, inclusive, comprised the sample. The majority of the subjects were Caucasian females, ages 20 to 24. The admissions criteria examined were (1) Dental Hygiene Aptitude Test scores, (2) Scholastic Aptitude Test scores, (3) preadmission quality point average, (4) college chemistry quality point average, and (S) percentile rank in …

Evaluation Of Peptide Assimilation In Ehrlich Ascites Tumor Cells, John M. Castellano

Biological Sciences Theses & Dissertations

The dipeptides glycyl-L-(³H)-leucine and L-phenylalanyl-L-(³H)-leucine were synthesized in radioactive form and utilized in the analysis of peptide transport and hydrolysis by Ehrlich ascites tumor cells. The tumor cells were cultured in the abdominal cavity of CF-1 albino mice and harvested into sterile isotonic buffer for all experimental analyses.

The results of this study reveal that the tumor cells rapidly accumulate radiolabel when incubated with the above mentioned peptides, but that the peptide is most probably hydrolyzed extracellularly and free L-(³)-leucine transported. This hypothesis is based on the observations of reduction in accumulation of radiolabel …