Medicine and Health Sciences Commons^™

Implementation Of Gy-Eq For Deterministic Effects Limitation In Shield Design, John W. Wilson, Myung-Hee Y. Kim, Giovanni De Angelis, Francis A. Cucinotta, Nobuaki Yoshizawa, Francis F. Badavi

Mathematics & Statistics Faculty Publications

The NCRP has recently defined RBE values and a new quantity (Gy-Eq) for use in estimation of deterministic effects in space shielding and operations. The NCRP's RBE for neutrons is left ambiguous and not fully defined. In the present report we will suggest a complete definition of neutron RBE consistent with the NCRP recommendations and evaluate attenuation properties of deterministic effects (Gy-Eq) in comparison with other dosimetric quantities.

Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola

Bioelectrics Publications

In vivo electroporation is an efficient means of increasing plasmid DNA delivery to normal tissues, such as skin and muscle, as well as directly to tumors. In the experiments described here, plasmid DNA was delivered by in vivo electroporation to B16 mouse melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up …

Electric Field Enhanced Plasmid Delivery To Liver Hepatocellular Carcinomas, Richard Gilbert, Mark J. Jaroszeski, Loree Heller, Richard Heller

Bioelectrics Publications

Electric field enhanced molecular delivery for cancer research and treatment is a new technology that has demonstrated its effectiveness in clinical trials using bleomycin or cisplatin (Heller, R., Gilbert, R., Jaroszeski, M. J. Clinical applications of electrochemotherapy, Advanced Drug Delivery Reviews 35,119-129, 1999), as chemotherapeutic agents. The technology is being investigated in research applications for applicability as a method to enhance gene expression in a target tumor. Success is predicated on an appropriate effective electric field mediated delivery protocol that triggers significant appropriate gene expression duration and levels. An electric field mediated delivery protocol includes a set of conditions …

Effects Of Occupational Ultrasonic Noise Exposure On Hearing Of Dental Hygienists: A Pilot Study, Jennifer Dunning Wilson, Michele Leonardi Darby, Susan L. Tolle, Joseph C. Sever Jr.

Dental Hygiene Faculty Publications

Purpose. The purpose of this case-control study was to determine whether long-term ultrasonic noise exposure via the dental office environment is related to dental hygienists' hearing status.

Methods. Registered dental hygienists (N - 698) who live in the Hampon Roads areas of Virginia were mailed a Dental Hygiene Word History Questionnaire to determine who would meet the inclusion criteria and would be willing to participate in the study. Consenting subjects were categorized into one of two groups, according to ultrasonic sealer usage rate, and matched on age. Persons with known hearing loss due to infection, disease, or congenital defect were …

Effect Of Electrically Mediated Intratumor And Intramuscular Delivery Of A Plasmid Encoding Ifn Α On Visible B16 Mouse Melanomas, Loree C. Heller, Stephanie F. Ingram, M. Lee Lucas, Richard A. Gilbert, Richard Heller

Bioelectrics Publications

Interferon α may be used as a single agent therapy for metastatic malignant melanoma or as an adjuvant to chemotherapy. Delivery of interferon α by gene therapy offers an alternative to recombinant protein therapy. Electrically mediated delivery enhances plasmid expression in a number of tissues, for instance skin, liver, muscle and tumors including melanomas. Here we compare the effect of delivery of a plasmid encoding mouse interferon α on growth of visible B16 mouse melanomas following electrically mediated delivery to muscle or directly to the tumor. Intratumoral delivery of interferon α plasmid not only slows melanoma growth, but induces complete, …

Evidence For Infanticide In Bottlenose Dolphins Of The Western North Atlantic, Dale G. Dunn, Susan G. Barco, D. Ann Pabst, William A. Mclellan

Biological Sciences Faculty Publications

Nine bottlenose dolphin (Tursiops truncatus) calves that stranded in Virginia in 1996 and 1997 died of severe blunt-force trauma. Injuries were concentrated on the head and chest and multiple rib fractures, lung lacerations, and soft tissue contusions were prominent. Skeletal and/or soft tissue trauma occurred bilaterally in all of the calves. One had a bite wound across the left mandible that exhibited deep punctures consistent with the tooth placement in an adult bottlenose dolphin. The lesions were not compatible with predation, boat strike, fisheries interactions, rough-surf injury, or blast injury. However, they were similar to traumatic injuries described in stranded …

Characterizing Consumer Motivation As Individual Difference Factors: Augmenting The Sports Interest Inventory (Sii) To Explain Level Of Spectator Support, Daniel C. Funk, Daniel F. Mahony, Lynn L. Ridinger

Human Movement Sciences & Special Education Faculty Publications

The central focus of this study was to examine how individual difference factors could be used to explain various levels of consumer support for a specific sport property. The present study extends the Sport Interest Inventory (SII) in order to enhance current understanding of consumer motives in relation to sport in general and women's competitive sport in particular. Confirmatory factor analysis confirmed the 12-item Sport Interest Inventory, measuring 14 individual difference factors related to spectator interest in soccer. Multiple Linear regression analysis revealed that five motivational characteristic--(a) sport interest, (b) team interest, (c) vicarious achievement, (d) role modeling, and (e) …

Il-12 Plasmid Delivery By In Vivo Electroporation For The Successful Treatment Of Established Subcutaneous B16.F10 Melanoma, M. Lee Lucus, Loree Heller, Domenico Coppola, Richard Heller

Bioelectrics Publications

Interleukin-12 (IL-12) has been used in numerous immunotherapy protocols against melanoma. However, delivery of IL-12 in the form of recombinant protein can result in severe toxicity, and gene therapy has had limited success against B16.F10 murine melanoma. The purpose of this study was to examine the effectiveness of in vivo electroporation for the delivery of plasmid DNA encoding IL-12 as an antitumor agent against B16.F10 melanoma. We treated mice bearing established B16.F10 melanoma tumors with intratumoral (i.t.) or intramuscular (i.m.) injections of a plasmid encoding IL-12, followed by in vivo electroporation. For i.t. treatments, we used an applicator containing six …

Improved Energy Model For Membrane Electroporation In Biological Cells Subjected To Electrical Pulses, R. P. Joshi, Q. Hu, K. H. Schoenbach, H. P. Hjalmarson

Bioelectrics Publications

A self-consistent model analysis of electroporation in biological cells has been carried out based on an improved energy model. The simple energy model used in the literature is somewhat incorrect and unphysical for a variety of reasons. Our model for the pore formation energy E(r) includes a dependence on pore population and density. It also allows for variable surface tension, incorporates the effects of finite conductivity on the electrostatic correction term, and is dynamic in nature. Self-consistent calculations, based on a coupled scheme involving the Smoluchowski equation and the improved energy model, are presented. It is shown that E(r) becomes …

Eating Disorders In The Oral Health Curriculum, Rita D. Debate, Deanne Shuman, Lisa A. Tedesco

Dental Hygiene Faculty Publications

Due to the oral/systemic nature of eating disorders, this serious health issue requires comprehensive patient assessment and coordinated health treatment. The purpose of this study was to assess the breadth and depth of eating disorder and comprehensive care within the dental and dental hygiene curriculum. Survey data were collected from deans of U.S. dental programs (n=24) and dental hygiene program directors (n=94). Statistically significant differences were observed between dental programs (DP) and dental hygiene programs (DHP) as more DHP reported including anorexia nervosa (p<.001), bulimia nervosa (p<.001), and oral manifestations of eating disorders (p=.003) within their curricula. Clock hours dedicated to these topics ranged from seventeen to thirty-five minutes, with no statistically significant differences observed between DP and DHP. Only 58 percent of DP and 56 percent of DHP included patient communication skills specific to eating disorders. Moreover, DHP were observed dedicating more instruction time for this skill (p=.011). As greater emphasis is placed on oral/systemic health and the provision of comprehensive care, many oral health professionals may not be adequately trained to identify, provide education, and communicate with patients regarding the oral/systemic nature of eating disorders. The findings from this study indicate that there is a need for appropriate training to better prepare oral health professionals for comprehensive patient care.

Vo2 Reserve And The Minimal Intensity For Improving Cardiorespiratory Fitness, David P. Swain, Barry A. Franklin

Human Movement Sciences & Special Education Faculty Publications

Purpose: The American College of Sports Medicine has stated that aerobic: training needs to occur at a minimum threshold intensity of 50% VO_2max for most healthy adults and at 40% VO_2max for those with a very low initial fitness. Recently, the concept of VO₂ reserve (% VO₂R, i.e., a percentage of the difference between maximum and resting VO₂ has been introduced for prescribing exercise intensity, This analysis was designed to determine the threshold intensity for improving cardiorespiratory fitness expressed as %VO₂R units. Methods: Previous studies in healthy subjects (N = 18) that …

Is There A Threshold Intensity For Aerobic Training In Cardiac Patients?, David P. Swain, Barry Franklin

Human Movement Sciences & Special Education Faculty Publications

Purpose: Recent guidelines have recommended the use of a percentage of oxygen uptake reserve ((V) over dot O₂R) for prescribing aerobic exercise intensity for cardiac patients. Moreover, these guidelines suggest that a threshold intensity may exist, below which no improvement in peak oxygen uptake ((V) over dot O-_2peak) occurs, The purpose. therefore. was to translate the intensity of aerobic exercise in previous training studies using cardiac patients into %(V) over dot O₂R units, and determine whether a threshold intensity exists, Methods: Twenty-three studies, using 28 groups of aerobically trained cardiac patients. were identified in …

Continuing Competence In Selected Health Care Professions, Burden S. Lundgren, Clare A. Houseman

Nursing Faculty Publications

Health services professionals are confronting the challenge of maintaining and improving competence over the course of lengthy careers in diverse practice specialties. This article reviews the efforts of a selection of health care professions to ensure lifetime competence and reviews some of the challenges encountered in these efforts. Although each profession has its own issues, significant generic questions are common to all.

How Physicians Feel About Assisting Female Victims Of Intimate-Partner Violence, Ramani N. Garimella, Stacey B. Plichta, Clare Houseman, Laurel Garzon

Community & Environmental Health Faculty Publications

Purpose. To assess the feelings of physicians about assisting female victims of intimate-partner violence (IPV), and to examine factors related to positive and negative feelings about assisting victims of IPV.

Method. In 1998, a total site sample of 150 physicians practicing in a large general hospital in the area of Virginia Beach, Virginia, was surveyed by questionnaire via the mail. Four specialties were represented: emergency medicine, family practice, obstetrics-gynecology, and psychiatry. The questionnaire asked about medical training and training in assisting victims of IPV The physicians' feelings about working with victims of IPV were measured on a nine-item, five-point …