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Old Dominion University

Series

1989

Articles 1 - 3 of 3

Full-Text Articles in Medicine and Health Sciences

Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little Oct 1989

Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little

Medical Diagnostics & Translational Sciences Faculty Publications

Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …

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Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little Jun 1989

Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little

Medical Diagnostics & Translational Sciences Faculty Publications

Several cell surface proteins (Mr = 120,000, 90,000, 63,000 and 47,000) apparently integral to embryonic fibroblast plasma membranes were extracted with detergent and isolated by collagen affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000, and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist on the embryonic fibroblast cell surface. Some of the proteins may be collagen receptors binding to RGD sequences in the collagen molecule while at least one of the proteins (Mr = 63,000) recognizes …

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House Bill 1024: A Chronology, Clare Houseman, Leslie Hurt, Lucy Smith, Michele Zimmerman Jan 1989

House Bill 1024: A Chronology, Clare Houseman, Leslie Hurt, Lucy Smith, Michele Zimmerman

Nursing Faculty Publications

In speaking to those assembled for Legislative Day, Tim Oksman, lobbyist for HB1024 stated that the bill's passage against all odds was a tribute to the respect that the legislators have for the Nursing Profession. For persons interested in the political process surrounding HB1024 this chronology is written. If at times it seems repetitive, it's because the process was. The same core group of people, give or take a couple, said the same things to different people over and over again. Perhaps it is important to emphasize both the frustration and necessity of this repetition and that patience is needed …

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