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Full-Text Articles in Medicine and Health Sciences
Adenoviral And Adeno-Associated Viral Vectors-Mediated Neuronal Gene Transfer To Cardiovascular Control Regions Of The Rat Brain, Yanling Zhang, Yongxin Gao, Robert C. Speth, Nan Jiang, Yingying Mao, Colin Sumners, Hongwei Li
Adenoviral And Adeno-Associated Viral Vectors-Mediated Neuronal Gene Transfer To Cardiovascular Control Regions Of The Rat Brain, Yanling Zhang, Yongxin Gao, Robert C. Speth, Nan Jiang, Yingying Mao, Colin Sumners, Hongwei Li
HPD Articles
Viral vectors have been utilized extensively to introduce genetic material into the central nervous system. In order to investigate gene functions in cardiovascular control regions of rat brain, we applied WPRE (woodchuck hepatitis virus post-transcriptional regulatory element) enhanced-adenoviral (Ad) and adeno-assoicated virus (AAV) type 2 vectors to mediate neuronal gene delivery to the paraventricular nucleus of the hypothalamus, the nucleus tractus solitarius and the rostral ventrolateral medulla, three important cardiovascular control regions known to express renin-angiotensin system (RAS) genes. Ad or AAV2 harboring an enhanced green fluorescent protein (EGFP) reporter gene or the angiotensin type 2 receptor gene were microinjected …