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Enhanced Protective Efficacy Of A Chimeric Form Of The Schistosomiasis Vaccine Antigen Sm-Tsp-2, Mark S. Pearson, Darren A. Pickering, Henry J. Mcsorley, Jeffrey M. Bethony, Leon Tribolet, Annette M. Dougall, Peter J. Hotez, Alex Loukas
Enhanced Protective Efficacy Of A Chimeric Form Of The Schistosomiasis Vaccine Antigen Sm-Tsp-2, Mark S. Pearson, Darren A. Pickering, Henry J. Mcsorley, Jeffrey M. Bethony, Leon Tribolet, Annette M. Dougall, Peter J. Hotez, Alex Loukas
Microbiology, Immunology, and Tropical Medicine Faculty Publications
The large extracellular loop of the Schistosoma mansoni tetraspanin, Sm-TSP-2, when fused to a thioredoxin partner and formulated with Freund's adjuvants, has been shown to be an efficacious vaccine against murine schistosomiasis. Moreover, Sm-TSP-2 is uniquely recognised by IgG1 and IgG3 from putatively resistant individuals resident in S. mansoniendemic areas in Brazil. In the present study, we expressed Sm-TSP-2 at high yield and in soluble form in E. coli without the need for a solubility enhancing fusion partner. We also expressed in E. coli a chimera called Sm-TSP-2/5B, which consisted of Sm-TSP-2 fused to …
The Adjuvanticity Of An O. Volvulus-Derived Rov-Asp-1 Protein In Mice Using Sequential Vaccinations And In Non-Human Primates, Jing Wang, Nancy Trichoche, Lanying Du, Meredith Hunter, Bin Zhan, Gaddam Goud, Elizabeth S. Didier, Jing Liu, Lu Lu, Preston A. Marx, Shibo Jiang, Sara Lustigman
The Adjuvanticity Of An O. Volvulus-Derived Rov-Asp-1 Protein In Mice Using Sequential Vaccinations And In Non-Human Primates, Jing Wang, Nancy Trichoche, Lanying Du, Meredith Hunter, Bin Zhan, Gaddam Goud, Elizabeth S. Didier, Jing Liu, Lu Lu, Preston A. Marx, Shibo Jiang, Sara Lustigman
Microbiology, Immunology, and Tropical Medicine Faculty Publications
Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the Onchocerca volvulusrecombinant protein rOv-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6. However, because it is a protein, the r …