Medicine and Health Sciences Commons^™

Palmitoylation Regulates Intracellular Trafficking Of Β2 Adrenergic Receptor/Arrestin/Phosphodiesterase 4d Complexes In Cardiomyocytes, Ruijie Liu, Dayong Wang, Qian Shi, Qin Fu, Steven Hizon, Yang K. Xiang

Peer Reviewed Articles

β₂ adrenergic receptor (β₂AR) is a prototypical G-protein coupled receptor that stimulates the classic cAMP-protein kinase A (PKA) signaling pathway. Recent studies indicate that the cAMP-PKA activities are spatiotemporally regulated in part due to dynamic association of β₂AR with phosphodiesterase 4D (PDE4D), a group of cAMP degradation enzymes. Here, we demonstrate that in cardiomyocytes, palmitoylation of β₂AR, the covalent acylation of cysteine residue 341, plays a critical role in shaping subcellular cAMP-PKA activities in cardiomyocytes via regulating β₂AR association with arrestin/PDE4D. Replacing cysteine 341 on β₂AR with alanine (C341A) …

How Can We Help Minority Nursing Students?, Nancy Schoofs

Peer Reviewed Articles

This article details the example of remediating an at-risk nursing student who failed two clinical rotations at a large university nursing program. It is a case of working with a minority student using six simulations and two clinical days in the hospital. The author describes the process of how the simulations and clinical experiences were used to assist the student in increasing her confidence and clinical abilities. In addition to the example of the student, an extensive literature review was done prior to working with the student. This aided the author not only in determining methods for assisting the student, …

Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis And Have Contrasting Effects On Chemotaxis And Apoptosis, Robert Boomsma, David Geenen

Peer Reviewed Articles

We have previously shown that mesenchymal stem cells (MSC) improve function upon integration in ischemic myocardium. We examined whether specific cytokines and growth factors produced by MSCs are able to affect angiogenesis, cellular migration and apoptosis. Conditioned media (CM) was prepared by culturing MSC for 48 hours. CM displayed significantly elevated levels of VEGF, Monocyte Chemoattractant Protein-1 (MCP-1), macrophage inflammatory protein-1a (MIP-1a), MIP- 1b and monokine induced by IFN-c (MIG) compared to control media. MSC contained RNA for these factors as detected by RT-PCR. CM was able to induce angiogenesis in canine vascular endothelial cells. MCP-1 and MIP-1a increased cell …

Fall Prevention In Hospitals: An Integrative Review, Sandra L. Spoelstra, Barbara A. Given, Charles W. Given

Peer Reviewed Articles

Purpose: This article summarize research and draws overall conclusions from the body of literature on fall prevention interventions to provide nurse administrators with a basis for developing evidence-based fall prevention programs in the hospital setting.

Method: Data were obtained from published studies. Thirteen articles were retrieved that focused on fall interventions in the hospital setting. An analysis was performed based on levels of evidence using an integrative review process.

Results: Multifactoral fall prevention intervention programs that included fall risk assessments, door/bed/patient fall risk alerts, environmental and equipment modifications, staff and patient safety education, medication management targeted to specific types, and …

Cardiac-Specific Expression Of The Tetracycline Transactivator Confers Increased Heart Function And Survival Following Ischemia Reperfusion Injury, Laila Elsherif, Xuerong Wang, Milana Grachoff, Beata Wolska, David Geenen, John O'Bryan

Peer Reviewed Articles

Mice expressing the tetracycline transactivator (tTA) transcription factor driven by the rat a-myosin heavy chain promoter (a-MHC-tTA) are widely used to dissect the molecular mechanisms involved in cardiac development and disease. However, these a-MHC-tTA mice exhibit a gain-of-function phenotype consisting of robust protection against ischemia/reperfusion injury in both in vitro and in vivo models in the absence of associated cardiac hypertrophy or remodeling. Cardiac function, as assessed by echocardiography, did not differ between a-MHC-tTA and control animals, and there were no noticeable differences observed between the two groups in HW/TL ratio or LV end-diastolic and end-systolic dimensions. Protection against ischemia/reperfusion …