Medicine and Health Sciences Commons^™

Mapping Next Generation Sequence Data With Bwa (Burrows-Wheel Aligner) On Galaxy Software, Rabeh Z. Omar

Honors College Theses

Advancement of next-generation sequencing technologies introduces a vast amount of data which has become a challenge for researchers to organize and sequence data sets. BWA (Burrows-Wheeler Aligner) is one of the widely used software for aligning and mapping sequencing data against a reference genome. In my thesis, I present a comprehensive guide for analyzing genome sequences using BWA. I discuss the various steps involved in the process, including gathering the data, preparing the reference genome, aligning the sequences, and processing the data to visualize the results.