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Heterologous Complementation Reveals That Mutant Alleles Of Qsr1 Render 60s Ribosomal Subunits Unstable And Translationally Inactive, Frederick A. Dick, Bernard L. Trumpower

Dartmouth Scholarship

QSR1 is a highly conserved gene which encodes a 60S ribosomal subunit protein that is required for joining of large and small ribosomal subunits. In this report we demonstrate heterologous complementation of a yeast QSR1 deletion strain with both the human and corn homologs and show that the human and corn proteins are assembled into hybrid yeast/human and yeast/corn ribosomes. While the homologous genes complement lethality of the QSR1 deletion, they also result in a diminished growth rate. Analyses of the translation rates of ribosomes containing the human and corn proteins reveal a partial loss of function. Velocity gradient analyses …