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Full-Text Articles in Medicine and Health Sciences
Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin
Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin
The Thai Journal of Veterinary Medicine
FCoV viruses exhibit great genetic diversity, leading to the presence of FIPV-causing variants. Current molecular evolution analysis and genetic variation studies of FCoV in China are predominately focused on gene encoding the spike protein or other structural proteins, while few studies have evaluated genetic variations in nonstructural FCoV genes, which can play an important role in disease pathogenesis. In this study, the gene encoding the open reading frame (ORF) 7b nonstructural FCoV protein of the Chinese Fujian strain FJLY20201 was amplified from the ascitic fluid of a Chinese domestic cat infected with FIPV and compared with ORF 7b from previously …
Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen
Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen
The Thai Journal of Veterinary Medicine
To identify further the infectivity, genetic diversity and molecular characteristics of the porcine epidemic diarrhea virus (PEDV). 286 samples of the intestinal tissue of pigs with severe diarrhea were assessed from 5 provinces in the north of Vietnam. The full-length ORF3 gene of 20 representative field strains from 36 farms in 5 provinces was sequenced and analyzed. The prevalence of PEDV was 90.56% and was detected in 259 of 286 samples, with 94.44% (34 of 36) of pig farms harboring the disease. Based on the phylogenetic analysis of the ORF3 genes, our isolates all fell into group G2 (variants) and …
Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China, Jing Yang, Ming Li, Tianfei Yu
Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China, Jing Yang, Ming Li, Tianfei Yu
The Thai Journal of Veterinary Medicine
Muscovy duck parvovirus (MDPV) is fatal for young ducklings of less than 3 weeks of age and has caused severe economic loss in duck production in many countries and regions. We report here on a complete genome sequence of a recombinant MDPV strain YL08 isolated from an ill Muscovy duck in 2008 at Qiqihar, Heilongjiang province, China. This genome, MDPV YL08, was 5061 nucleotides long and retained the two ORFs, which are typical in the Parvovirinae family. The genome of YL08 strain has a putative recombination breakpoint on its VP1 gene. This study has helped our understanding of the genetic …
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan, Maria Komal, Kiran Afshan, Sabika Firasat, Màrius V. Fuentes
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan, Maria Komal, Kiran Afshan, Sabika Firasat, Màrius V. Fuentes
The Thai Journal of Veterinary Medicine
This study determined the molecular characterization of Paramphistomum epiclitum using the second internal transcribed spacer (ITS-2) grouping and secondary structure analysis from buffaloes in Khyber Pakhtunkhwa province, Pakistan. Paramphistomes were collected from the rumen of 25 infected buffaloes and their DNA was separated. DNA was intensified, followed by sequencing. Phylogenetic examinations were carried out using the Maximum Composite Likelihood approach. The results revealed extensive intraspecific and interspecific varieties among different paramphistomid species. The sequences in the present study (GenBank accession number: MW481321 and MW481322) were compared to 46 reference sequences of Paramphistomum and other trematode data from GenBank. The results …
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China, Yulong Zhou, Guoqiang Gao, Liang Zheng, Zhijun Wu, Hongwei Cao, Hua Zhang, Yanlong Cong
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China, Yulong Zhou, Guoqiang Gao, Liang Zheng, Zhijun Wu, Hongwei Cao, Hua Zhang, Yanlong Cong
The Thai Journal of Veterinary Medicine
In this present study, 11 porcine epidemic diarrhea viruses (PEDVs) were isolated from the fecal samples of piglets infected with PEDV in HeiLongJiang province of China. In order to analyze the molecular evolution of these reemerging PEDVs, the ORF3 and M genes of 11 PEDVs were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed with each other as well as other PEDV reference strains. The complete nucleotide sequence comparison was performed with Bioedit software, and the results showed that the similarity of 11 ORF3 and M genes ranged from 97% to 100%. Nucleotides and amino acids were …
Sequence And Phylogenetic Analysis Of The Vp1 Segment Of Chicken Infectious Anemia Virus (Ciav) During An Outbreak In Palestine, Ibrahim Alzuheir, Nasr Jalboush, Adnan Fayyad, Mohammad Manassra
Sequence And Phylogenetic Analysis Of The Vp1 Segment Of Chicken Infectious Anemia Virus (Ciav) During An Outbreak In Palestine, Ibrahim Alzuheir, Nasr Jalboush, Adnan Fayyad, Mohammad Manassra
The Thai Journal of Veterinary Medicine
In this study, chicken infectious anemia virus (CIAV) was detected in two unvaccinatedcommercial broiler flocks, showing clinical signs relevant to Chicken infectious anemia (CIA) disease. The clinical signs included subcutaneous hemorrhage, depression and death. Necropsy findings were pale liver, severe atrophy of bursa of the Fabricius and thymus and discoloration of the bone marrow. DNA was extracted from the livers of the infected birds and the VP1 gene was detected by polymerase chain reaction. The virus strains were detected and genetically characterized in almost the whole VP 1 gene (1338 base pairs). The nucleic acid analysis showed that the two …
Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li
Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li
The Thai Journal of Veterinary Medicine
Trichuris ovis (Nematoda: Trichuridae) is a widespread nematode that can infect a broad range of animal hosts, such as goats, sheep and oryx. This parasite is of vital importance as a pathogen, but its population genetics, molecular epidemiology and biology are still not well understood. In this study, T. ovis isolates were collected from goats in Hunan province, China, and sequences variation in cox1 gene were examined. A portion of cox1 gene from each collected adult T. ovis individual was separately amplified using the PCR method, and the amplicons were sequenced from both directions. The sequences of pcox1 gene were …
Molecular Characterization Of Common Respiratory Viral Infections In Broilers In Al-Hassa, Eastern Province, Saudi Arabia, Ahmed M. Al-Ali, Ibrahim M. El-Sabagh, Mahmoud H. Mohamed, Ahmed M. Alluwaimi, Ibrahim A. Arif
Molecular Characterization Of Common Respiratory Viral Infections In Broilers In Al-Hassa, Eastern Province, Saudi Arabia, Ahmed M. Al-Ali, Ibrahim M. El-Sabagh, Mahmoud H. Mohamed, Ahmed M. Alluwaimi, Ibrahim A. Arif
The Thai Journal of Veterinary Medicine
Newcastle disease virus (NDV), Infectious bronchitis virus (IBV) and H9N2 avian influenza virus (AIV) are the most significant respiratory pathogens. Several outbreaks have been reported frequently in Saudi Arabia despite of the intensive use of vaccination programs. Ten broiler flocks suffering from complicated respiratory disease were subjected to necropsy and samples were collected for molecular identification of involved viruses and pathogenic bacterial infections. Seven out of the ten flocks were positive for single or combined viral infection. The seven flocks were positive for HA test. All positive samples were subjected for molecular identification, three flocks were positive for single NDV …
Molecular Characteristics Of Vp2 Gene From Wild-Type Infectious Bursal Disease Viruses (Ibdvs) In Saudi Arabia, Mahmoud H A Mohamed, Mahmoud M. Ismail, Ibrahim M. El-Sabagh, Abdul-Rahman S. Al-Ankari
Molecular Characteristics Of Vp2 Gene From Wild-Type Infectious Bursal Disease Viruses (Ibdvs) In Saudi Arabia, Mahmoud H A Mohamed, Mahmoud M. Ismail, Ibrahim M. El-Sabagh, Abdul-Rahman S. Al-Ankari
The Thai Journal of Veterinary Medicine
Infectious bursal disease (IBD) is caused by a virus related to the family Birnaviridae. The virus is a worldwide pathogen and causes immunosuppression and great losses in broiler flocks. In this study, molecular characterization of two Saudi Arabian IBDVs (IBD01/14/SA and IBD02/14/SA) was carried out by reverse transcriptase polymerase chain reaction (RT-PCR) and partial sequence of hypervariable region of VP2 gene. Nucleotide sequences of 740-bp PCR product of the Saudi Arabian isolates were compared with the corresponding nucleotide sequence of 56 reported IBDVs representing different pathotypes from different continents. Phylogenetic analysis showed that the Saudi Arabian isolates were split from …
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
The Thai Journal of Veterinary Medicine
Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. In the current study, genetic identification of sapovirus (SaV) in suckling piglets was investigated in Hunan province, China between May 2013 and May 2014. Diarrheic fecal (n=300) samples collected from suckling piglets in Hunan province, China were evaluated using RT-PCR and then sequenced. Overall, 45 of the 300 (15%) samples were found to contain porcine SaV. In addition, phylogenetic analysis showed that all the porcine SaV isolates belonged to the porcine SaV genogroup III (GIII). The results of the present investigation have implications for the control of …
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
The Thai Journal of Veterinary Medicine
Wenyonella philiplevinei is one of the important pathogens of duck coccidiasis. In the present study, a portion of the 18S rDNA was amplified from W. philiplevinei by Polymerase chain reaction (PCR), cloned and then sequenced. The sequences of 18S rDNA of all samples were 422 bp in size. A + T content of the 18S rDNA sequences was 58-59%. Sequence comparison revealed that the similarity in 18S rDNA sequences between Hunan isolates and W. philiplevinei available (Guangdong isolate) was more than 98%. Intra-specific sequence variations within each of the Hunan isolates were 0-1.7%. However, the inter-specific sequence differences between W. …
Molecular Detection And Genotype Differentiation Of Feline Coronavirus Isolates From Clinical Specimens In Thailand, Somporn Techangamsuwan, Araya Radtanakatikanon, Suphasawatt Purnaveja
Molecular Detection And Genotype Differentiation Of Feline Coronavirus Isolates From Clinical Specimens In Thailand, Somporn Techangamsuwan, Araya Radtanakatikanon, Suphasawatt Purnaveja
The Thai Journal of Veterinary Medicine
Feline coronavirus (FCoV) infection manifests a wide magnitude of clinical symptoms from nonpathogenic mild enteric infection to pathogenic fatal feline infectious peritonitis (FIP). Based on the in vitro properties and antigenic relationship to canine coronavirus (CCoV), FCoVs are classified into type I and II with different continent preference. To investigate the incidence of FCoV infection among cat populations in Thailand during 2010-2011, clinical specimens (103 bodily fluids from 95 FIP clinically suspected cats and 17 feces from 7 healthy cats living in groups and 10 healthy cats living singly) were analyzed by amplification of the 3’UTR gene. Among them, 46% …
Genetic Characterization And Phylogenetic Analysis Of Porcine Circovirus Type 2 In Thai Pigs With Porcine Circovirus Associated Diseases (Pcvad) During 2007-2010, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Roongroje Thanawongnuwech
Genetic Characterization And Phylogenetic Analysis Of Porcine Circovirus Type 2 In Thai Pigs With Porcine Circovirus Associated Diseases (Pcvad) During 2007-2010, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) was first recognized as a causative agent of post weaning multisystemicsyndrome or PMWS in Canada in 1991. Subsequently, it has been reported in almost all intensive pig productioncountries, and currently all associated diseases linked to PCV2 are included under the term porcine circovirusassociated diseases or PCVAD. PCV2 can be divided into 2 major genotypes referred to genotype 1 (PCV2b) andgenotype 2 (PCV2a). In Thailand, PMWS was first reported in 1998. However, only one isolate of Thai PCV2 from ourgroup has been submitted to GenBank (AY864814). Therefore, the objective of this study was to determine thegenetic …
Genetic Characterization Of Porcine Epidemic Diarrhea Virus (Pedv) Isolates From Southern Vietnam During 2009-2010 Outbreaks, Do Tien Duy, Tat Toan Nguyen, Suphasawatt Puranaveja, Roongroje Thanawongnuwech
Genetic Characterization Of Porcine Epidemic Diarrhea Virus (Pedv) Isolates From Southern Vietnam During 2009-2010 Outbreaks, Do Tien Duy, Tat Toan Nguyen, Suphasawatt Puranaveja, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine epidemic diarrhea virus (PEDV) spike (S) glyprotein and membrane (M) glycoprotein genes arebelieved to have genetic variation. The heterogeneity in those genomic sequences has been reported and is knownessentially for the diverse PEDV pathogenicity. Eight southern Vietnamese PEDVs collected from severe waterydiarrhea piglets of the recent emerging outbreaks (2009-2010) were sequenced and analyzed. The results revealedhigh nucleotide homology of the partial S gene of the current isolates at 98.9-100% and 99.7-100% identity of the fullM gene among these isolates despite dividing into two subclusters of different provincial origins. It should be notedthat the Vietnamese PEDVs contained high differences on …