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Full-Text Articles in Medicine and Health Sciences
Expression Of Prostaglandin F2 Alpha Receptors (Ptgfr) And C-Reactive Protein (Crp) In The Canine Uterus And Cervix With Opened- And Closed-Cervix Pyometra, Narong Tiptanavattana, Pokchon Khirilak, Theerawat Tharasanit, Natapol Duangtum, Kijsopon Jankaew, Prawit Sae-Jia, Nongnapat Kaewchana
Expression Of Prostaglandin F2 Alpha Receptors (Ptgfr) And C-Reactive Protein (Crp) In The Canine Uterus And Cervix With Opened- And Closed-Cervix Pyometra, Narong Tiptanavattana, Pokchon Khirilak, Theerawat Tharasanit, Natapol Duangtum, Kijsopon Jankaew, Prawit Sae-Jia, Nongnapat Kaewchana
The Thai Journal of Veterinary Medicine
Pyometra is a common reproductive disorder in female dogs. This study investigated the protein expression of prostaglandin F2 alpha receptors (PTGFR) and C-reactive protein (CRP) in responses to inflammatory events of uterine and cervical tissues in dogs. Uterus and cervix samples were collected from six dogs which had closed-cervix pyometra (n = 3) and opened- cervix pyometra (n = 3). Healthy dogs in diestrus (n = 3) and anestrus (n=3) were used as controls. Tissues were histologically prepared and stained with hematoxylin and eosin. Immunohistochemistry (IHC) and western blot analysis for the PTGFR and CRP were used to verify the …
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …
Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici
Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici
The Thai Journal of Veterinary Medicine
In this study, the possible protective effect of red radish containing total phenolic content and antioxidant components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity, triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated in CCl4induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw); while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was …
Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile
Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile
The Thai Journal of Veterinary Medicine
A mature intracranial teratoma was identified in a 5-month-old pet rabbit showing a sudden onset of obtundation, recumbency, flaccid tetraplegia, and hyporeflexia. A space-occupying suprasellar, irregular cystic mass extending from the thalamus to the brainstem was observed at necropsy. Histologically, the tumor was composed of irregular, multilocular cysts lined by a thin wall and interposed tissue where a heterogeneous collection of structures including adipose and myxomatous tissues, cartilage, glandularand ductal structures, skeletal muscle, nervous tissue, nerve fascicles, sebaceous glands, and exocrine pancreatic cells were recognized. Immunohistochemistry showed labeling of the diverse tissues with cytokeratin, vimentin, smooth muscle actin, GFAP, NSE, …
Concise Immunohistochemistry In Carcinoma Of Unknown Primary Origin, Chinnawut Suriyonplengsaeng
Concise Immunohistochemistry In Carcinoma Of Unknown Primary Origin, Chinnawut Suriyonplengsaeng
Chulalongkorn Medical Journal
Carcinoma of unknown primary origin is a malignant epithelial neoplasm clinically defined by the presence of metastasis without known primary origin at the time of diagnosis. When it is clinically encountered, further investigations should be considered. Identification of primary origin of such neoplasm is crucial for proper patient management and prognosis. Immunohistochemistry has become an ancillary study for resolving this issue. Initialimmunohistochemistry panel including AE1/AE3, S100, CD45 and vimentin is suggested for identification of lineage of tumor cell differentiation. If the tumor cells are diffusely positive for AE1/AE3 confirming the diagnosis of carcinoma, additional immunohistochemistry markers including CK7, CK20 and …
Study Of Testicular Immunolocalization Of Inhibin Subunits And Epididymal Histological Structures Among Different Antler Status In Rusa Deer (Rusa Timorensis), Apichaya Sudsukh, Kazuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Nikorn Thongtip
Study Of Testicular Immunolocalization Of Inhibin Subunits And Epididymal Histological Structures Among Different Antler Status In Rusa Deer (Rusa Timorensis), Apichaya Sudsukh, Kazuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Nikorn Thongtip
The Thai Journal of Veterinary Medicine
The number of rusa deer (Rusa timorensis) raising farms has highly increased in Thailand. Basic data on the reproduction of rusa deer are essential for improvement in deer farming reproduction and the assisted reproductive technologies (ARTs) of endangered deer species. This study aimed to determine the relation of antler changes to testicular and epididymis histological structure and cellular source of testicular inhibin subunits in rusa deer. Eighteen rusa deer testes and epididymis were collected from a slaughterhouse located in Kamphaeng Saen, Nakhon Pathom, Thailand. The male rusa deer used in this study were separated into three groups depending on antler …
Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong
Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
Our previous study reported the expression of kisspeptin in the preoptic area (POA) and arcuate nucleus (ARC) of the hypothalamus of cycling buffalo cows. In ruminants, POA and ARC are the main hypothalamic nuclei through which kisspeptin acts on the hypothalamic-pituitary-gonadal axis, due to their stimulation of gonadotropin releasing hormone (GnRH) secretion. In many species, kisspeptin activates GnRH neurons via its receptors (KISS1R or GPR54). The present study was designed to explore the expression of KISS1R by GnRH neurons in the POA and ARC hypothalamic nuclei in buffalo cow brains by double-labeling immunohistochemistry. In both the POA and ARC areas, …
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.
Histopathological And Immunohistochemical Characterization Of Spontaneous Uterine Leiomyomas In Two Captive Asian Elephants, Kidsadagon Pringproa, Hiroo Madarame, Jiraporn Sritun, Pitikarn Bumpenpol, Paninee Pedsri, Chalermchart Somgird, Chatchote Thitaram
Histopathological And Immunohistochemical Characterization Of Spontaneous Uterine Leiomyomas In Two Captive Asian Elephants, Kidsadagon Pringproa, Hiroo Madarame, Jiraporn Sritun, Pitikarn Bumpenpol, Paninee Pedsri, Chalermchart Somgird, Chatchote Thitaram
The Thai Journal of Veterinary Medicine
Two captive female Asian elephants (Elephas maximus) in Thailand were euthanatized after prolonged recumbency, weakness, and unresponsive treatment. Necropsy revealed multinodular masses of 5-10 cm in diameter which were irregular, large, white and firm in consistency scattered throughout the uterine horns and uteri of both the elephants. Histologically, the tumors displayed mildly pleomorphic, spindle-shaped cells arranged in interlacing pattern interpreted as muscle bundles. The bundles of the muscle cells were interspersed by small amounts of collagen fibers and blood vessels. For immunohistochemistry, the tumor cells showed diffuse positivity for vimentin, desmin, and smooth muscle actin. Based on the pathological results, …
Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar
Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar
The Thai Journal of Veterinary Medicine
Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the …
Distribution Of Edwardsiella Tarda Antigens And Igm Containing Cells In Tilapia Immune Organs During Septicemia: An Immunohistochemical Study, Nopadon Pirarat, Takayuki Katagiri, Masashi Maita, Makoto Endo, Achariya Sailasuta
Distribution Of Edwardsiella Tarda Antigens And Igm Containing Cells In Tilapia Immune Organs During Septicemia: An Immunohistochemical Study, Nopadon Pirarat, Takayuki Katagiri, Masashi Maita, Makoto Endo, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
An immunohistochemical study describes the localization of IgM containing cells involved in the primary immune organs of tilapia and the fate of E. tarda antigen distributed in the tissues during a course of intraperitoneal challenge. The E. tarda antigen was diffusely detected in various organs except the brain and spinal cord. The positive signals were identified within various cell types, including phagocytic cells, serosal mesothelia, myocardia, intestinal and gill epithelia and glomerular capillary endothelia. No extracellular deposition of bacterial antigen was observed. The bacterial antigen can persist up to 1 month in the granuloma and melano-macrophage center, confirming the intracellular …
Pathological Study Of Helicobacter Spp. Infection In Pig Stomachs, Nopadon Pirarat, Veerasak Sada, Supradit Wangnaitham, Boonmee Sunyasootcharee
Pathological Study Of Helicobacter Spp. Infection In Pig Stomachs, Nopadon Pirarat, Veerasak Sada, Supradit Wangnaitham, Boonmee Sunyasootcharee
The Thai Journal of Veterinary Medicine
pathological study of Helicobacter spp. infection in pigs was performed to determine the incidence of Helicobacter spp. infection, and to define the relationship between Helicobacter spp. infection and the histopathological changes of gastric mucosa in pigs, using Warthin Starry stain and immunohistochemistry. A total of 115 biopsies of the cardia, fundus, body and pyloric antrum of swine (mean weight 150 kg) obtained from slaughterhouses in Nakornpathom province (Thailand) were investigated. Histopathological results of the cardia region showed thickening of mucosa 54.78% (63/115), vacuolated degeneration of the epithelium 83.47% (96/115), lymphoid aggregation 74.78% (86/115), inflammatory cells infiltration 38.26% (44/115) and neovascularized …
A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong
A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong
The Thai Journal of Veterinary Medicine
Ovarian steroid hormones and their receptors play an important role in both physiological as well as pathological changes seen in the uterus. For the further understanding of the mechanism of pyometra, in relation to steroid hormones and their receptors in the bitch, the difference between the expression of the oestrogen receptor alpha (ERa) between normal and pyometra bitches during dioestrus was investigated. Uterine samples were collected during dioestrus from normal and pyometra bitches. Immunohistochemistry was used to investigate the expression of ERa in the different compartments of the uterus. The results showed that the levels of steroid hormones in the …
An Immunohistochemical Study Of The Progesterone Receptor (Pr) Expression In Pyometra Cases And Normal Uteri At The Time Of Dioestrus In The Bitch, Kongkiat Srisuwatanasagul, Sayamon Sukjumlong, Sudson Sirivaidyapong, Sinchai Pianchop
An Immunohistochemical Study Of The Progesterone Receptor (Pr) Expression In Pyometra Cases And Normal Uteri At The Time Of Dioestrus In The Bitch, Kongkiat Srisuwatanasagul, Sayamon Sukjumlong, Sudson Sirivaidyapong, Sinchai Pianchop
The Thai Journal of Veterinary Medicine
The aims of the present study were to investigate the expression of the progesterone receptor (PR) and to compare whether there was any difference between pyometra cases and normal bitch uteri, during dioestrus, by means of immunohistochemistry. The uterine samples were collected from bitches which clients had submitted for spaying and for pyometra treatment. The results showed that a difference was obvious in the connective tissue stroma and myometrium in which a higher expression of PR was observed in the pyometra group.It may be postulated that the difference in PR expression in these uterine compartments may be involved in the …
The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat
The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat
The Thai Journal of Veterinary Medicine
The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade II, 41.46% but there was no statistically significant difference between them. An evaluation of a proliferative marker shown by a mean (±SD) of PCNA, was 34.63±23.87 and the mean (±SD) of AgNORs was 1.23±0.18. There was no significant difference between the PCNA and AgNORs index of both the histological types and the histological malignancy grades. However, a statistical difference of mean PCNA and AgNORs indices was noted in both c-erbB2 positive and c-erbB2 negative groups. The mean PCNA indices and AgNORs indices in c-erbB2 positive samples were 26.52±18.68 and 1.12±0.27 respectively, while the c-erbB2 negative ones were 39.43±26.24 and 1.28±0.19 respectively. In conclusion, c-erbB2 oncogene protein expression is considered to be an effective prognostic factor for adenocarcinoma subtypes of feline mammary tumors as it is in human and canine cases. However, integration of all the parameters (c-erbB2 oncogene protein, PCNA index, AgNORs index, histological types and clinical data) may achieve a more precise prognosis in feline, mammary tumor cases.
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …
Immunohistochemical Studies Of The Estrogen Receptor Alpha In The Ovaries And Uteri Of The Domestic Cat At Different Stages Of The Oestrous Cycle, Damri Darawiroj, Kongkiat Srisuwatanasagul, Sinchai Pianchop, Sayamon Sukjumlong
Immunohistochemical Studies Of The Estrogen Receptor Alpha In The Ovaries And Uteri Of The Domestic Cat At Different Stages Of The Oestrous Cycle, Damri Darawiroj, Kongkiat Srisuwatanasagul, Sinchai Pianchop, Sayamon Sukjumlong
The Thai Journal of Veterinary Medicine
The expression of the estrogen receptor alpha (ERα) was investigated in domestic cat ovaries and uteri by mean of immunohistochemistry, using monoclonal antibody against ERα. The stages of the oestrous cycle were determined by vaginal cytology and serum steroid levels which were taken before operation. In the uterus, positive staining was found in the nuclei of all uterine cell types in different proportions and staining intensity. During oestrous, the staining intensity and the proportion of positive cells were highest in the epithelium (both the surface epithelium and the glandular epithelium) as well as in the myometrium. At dioestrous, ERα expression …
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
The Thai Journal of Veterinary Medicine
An immunohistochemical examination of aborted bovine tissues, collected from two dairy farms that had a high abortion rate (7%), detected the organism Neospora caninum (NC). Five out of 12 aborting cows (41.7%) were seropositive to NC using a competitive ELISA test. Neospora-tachyzoites were detected in the placenta of a seropositive aborting cow. Neither NC parasites nor cysts were detected in other fetal tissues. The identification of the Neospora-parasite and a high percentage of Neospora seropositive aborting cows, suggested NC as the possible cause of abortion. This is the first identification of the NC parasite in Thailand.
The Expression Of Oestrogen And Progesterone Receptors In The Gilt Uterus At Different Stages Of The Oestrous Cycle, Sayamon Sukjumlong, Kongkiat Srisuwatanasagul, Adisorn Adirekthaworn, Kriengyot Sajjarengpong
The Expression Of Oestrogen And Progesterone Receptors In The Gilt Uterus At Different Stages Of The Oestrous Cycle, Sayamon Sukjumlong, Kongkiat Srisuwatanasagul, Adisorn Adirekthaworn, Kriengyot Sajjarengpong
The Thai Journal of Veterinary Medicine
Ovarian steroid hormones are known to be an important modulator in the regulation of reproductive function in the female. The levels of oestrogen and progesterone have been well documented whilst interacting during the entire oestrous cycle but their specific receptors in the target cells are less understood. Therefore, a comparative study of oestrogen (ER) and progesterone receptors (PR) at certain oestrous stages should be of help in the prediction of their interaction in specific uterine compartments. Tissue samples were collected at different stages of the oestrous cycle: oestrus (n=3), early dioestrus (n=3) and late dioestrus (n=3). They were fixed in …
Pituitary Adenoma In A Horse: A Case Report, Nopadon Pirarat, Kohsaku Chijiwa, Pattama Ritruechai, Sawang Kesdangsakonwut, Komkrich Teankum, Wijit Banlunara, Anudep Rungsipipat, Boonmee Sunyasutjaree
Pituitary Adenoma In A Horse: A Case Report, Nopadon Pirarat, Kohsaku Chijiwa, Pattama Ritruechai, Sawang Kesdangsakonwut, Komkrich Teankum, Wijit Banlunara, Anudep Rungsipipat, Boonmee Sunyasutjaree
The Thai Journal of Veterinary Medicine
A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, high fever, dysuria, nasal discharge and moist rale lung sounds. Gross examination revealed a single, white, firm, pituitary mass with a diameter 2.5 x 2.1 x 1.5 cm, compressed and overlying the hypothalamus. Histopathological and immunohistochemical examinations revealed the characteristic of a ACTH-derived pituitary adenoma.
A Retrospective Immunohistochemistry Study On Feline Panleukopenia Virus, Induced Enteritis, In Cats, Nopadon Pirarat, Theerayuth Kaewamatawong, Somporn Techangamsuwan
A Retrospective Immunohistochemistry Study On Feline Panleukopenia Virus, Induced Enteritis, In Cats, Nopadon Pirarat, Theerayuth Kaewamatawong, Somporn Techangamsuwan
The Thai Journal of Veterinary Medicine
The retrospective study of 30 cases records using formalin-fixed, paraffin-embedded intestines, from Feline Panleukopenia (FPL) infected cats using histopathological criteria as seen by the Department of Pathology, Faculty of Veterinary Sciences, Chulalongkorn University between 1997-2001, was undertaken. The objective of the study was to examine FPLV infection in feline intestines showing characteristic lesions of parvovirus enteritis and to describe the relationship between the intestinal microscopic lesions of FPL in affected cats, with the location of FPL antigens, using immunohistochemical methods. The most striking histological lesions were villous atrophy and epithelial cell necrosis 96.67%. The lumen of crypts were often greatly …
Immunohistochemical Detection Of Feline Viral Rhinotracheitis In A Cat, Nopadon Pirarat, Yumi Une, Yasuo Nomura
Immunohistochemical Detection Of Feline Viral Rhinotracheitis In A Cat, Nopadon Pirarat, Yumi Une, Yasuo Nomura
The Thai Journal of Veterinary Medicine
A female, 10-month old, mixed breed cat showed clinical signs of severe dehydration, purulent nasal discharge, dyspnea, severe crepitating lung sounds and no response to antibiotic treatment. Upon necropsy, histopathology revealed severe, diffuse, fibrinopurulent pleuropneumonia and numerous, homogeneous, amphophilic, intranuclear inclusion bodies in the pulmonary alveolar macrophages, bronchial and alveolar epithelium and bronchial glandular epitheliums. Immunohistochemical studies using 3 different monoclonal, anti-Feline Herpesvirus type 1 antibodies, revealed immunoreactivity of viral antigen in the lung that was associated with the histopathological changes. Monoclonal, anti-glycoprotein B, antibody showed the strongest immunoreactivity.
Soft Tissue Sarcoma At King Chulalongkornmemorial Hospital : A Review Of 105 Cases, L. Sansopha, V. Punyavoravut
Soft Tissue Sarcoma At King Chulalongkornmemorial Hospital : A Review Of 105 Cases, L. Sansopha, V. Punyavoravut
Chulalongkorn Medical Journal
No abstract provided.
The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura
The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura
The Thai Journal of Veterinary Medicine
Formalin fixed tissues from an aborted foal which had been diagnosed as having Equine Herpesvirus (EHV) infection, on histopathological criteria, were examined for EHV antigen using immunohistochemistry. EHV antigen was found in the focal necrotic area of the liver, especially in hepatocytes and Kupffer cells. Intranuclear and intracytoplasmic, immuno-positive labelings were also prominent in bronchiolar epithelial cells. The method allowed the visualization of the antigenic site distribution and their subsequent correlation with histopathological changes.
The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Thai Journal of Veterinary Medicine
The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa …
The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida
The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida
The Thai Journal of Veterinary Medicine
The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …
Small Intestinal Adenocarcinoma In A Dog: A Case Report, Saroj Chanyapat, Saravuth Konboon, Anudep Rungsipipat, Roongroje Thanawongnuwech
Small Intestinal Adenocarcinoma In A Dog: A Case Report, Saroj Chanyapat, Saravuth Konboon, Anudep Rungsipipat, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
A9-year-old female Shih Tzu dog was presented with a 3-day history of anorexia, depression, vomiting, and watery diarrhea. The clinical symptoms progressed continuously until the dog died, 3 days after admission. At necropsy numerous white nodules of various sizes were found in the small intestine, particularly the ileum. Microscopically, the mass revealed diffused, infiltrative growth of a well-differentiated adenocarcinoma invading the submucosa and muscular layers of the small intestinal wall. The mass was characterized by an acinar or tubular pattern with mucin in the lumen. The tumor cells were atypical cuboidal pleomorphic columnar epithelial cells with hyperchromatic round medium-sized nuclei …
Precore Mutants ของไวรสตับอักเสบ บี, P. Tangkijvanich, Y. Poovorawan
Precore Mutants ของไวรสตับอักเสบ บี, P. Tangkijvanich, Y. Poovorawan
Chulalongkorn Medical Journal
No abstract provided.
Immunohistochemistry ของ Trigeminal Primary Afferents ในงูเขียวหางไหม้, อรสิริ ชื่นทรวง, ริชาร์ด มอริส
Immunohistochemistry ของ Trigeminal Primary Afferents ในงูเขียวหางไหม้, อรสิริ ชื่นทรวง, ริชาร์ด มอริส
The Thai Journal of Veterinary Medicine
การศึกษาในครั้งนี้ทำการวิเคราะห์ด้วยวิธีทาง immunohistochemistry เพื่อศึกษาชนิดของneuro- transmitters ที่อาจเกี่ยวข้องกับการนำกระแสประสาทรับความรู้สึกเจ็บปวด และอุณหภูมิ (nociceptive และ thermoreceptive information) luusta trigeminal ganglion a sensory trigeminal nucleus vos medulla oblongata ของงูเขียวหางไหม้ (Trimeresurus albolaris) นำสมองงูเขียวหางไหม้จำนวน 12 ตัว มาแยกเอา brainstem และ maxillomandibular ganglion มาตัด section แล้วทำ immunofluorescent labelling โดยใช้ antibodies ต่อ neurotransmitters 6 ชนิด คือ substance P, calcitonin gene-related peptide (CGRP), galanin, neuropeptide Y, nitric oxide synthase (NOS) และ Somatostatin จากผลการทดลองพบว่า ใน ganglion มีเซลล์ขนาดเล็กถึงขนาดกลางจำนวนหนึ่ง ที่แสดง immunoreactivity ต่อ substance P และ CGRP และแทบจะไม่พบเซลล์ที่ให้ผลบวกต่อ galanin ในส่วนของ medulla oblongata WU substance P immunoreactivity lu fibres oglu descending trigeminal tract uazlu neuropil รอบ ๆ lateral descending nucleus ของ trigeminal nerve และยังพบได้ใน fibres ที่วิ่งพาดข้าม trigeminal subnucleus caudalis …