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Full-Text Articles in Medicine and Health Sciences
Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit
Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit
The Thai Journal of Veterinary Medicine
Bone marrow was collected from 7 dogs and submitted to 3 different MSC isolation techniques (direct plating, red blood cell lysis and gradient density). The number of cells, expression of MSC markers and in vitro the osteogenic differentiation obtained from each technique were examined. In order to study in vivo the osteogenic capability of derived MSCs, non-union ulna lesions (n=3) were firstly induced by transplantation of a composite of polycarprolactone/ hydroxyapatite (PCL/HAp) scaffold experimentally on to a critical-size ulna bone defect. The MSCs were injected into the lesions. The non-union sites were examined by radiography, angiography and histology at 2, …
Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh
Monitoring Il-13 Expression In Relation To Mirna-155 And Mirna-133 Changes Following Intra-Tracheal Administration Of Mesenchymal Stem Cells And Conditioned Media In Ovalbuminsensitized Rats, Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Rana Keyhanmanesh
The Thai Journal of Veterinary Medicine
The overall goal of the current study was to show possibly mechanisms by which mesenchymal stem cells (MSCs) or conditioned media (CM) exert immune-modulatory properties for amelioration of asthmatic changes. Male rats were classified into healthy (C) and ovalbumin-sensitized groups (S), which further were divided into three subgroups (n=6); rats given intratracheally PBS (CPT and SPT groups), CM (CST and SST groups) and MSCs (CCT and SCT groups). Two weeks post treatment, IL-13, miRNA- 133 and miRNA-155 transcripts, pathological injuries and the homing of MSCs into the lung parenchyma were evaluated. The local administration of CM and notably MSCs blunted …