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Influence Of Intracellular Glutamine Depletion On Regulation Of Amino Acid Transport System A In Placental (Bewo) Cells, Boonrit Thongsong
Influence Of Intracellular Glutamine Depletion On Regulation Of Amino Acid Transport System A In Placental (Bewo) Cells, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Glutamine is known to modulate the expression of various genes and uptake activity of nutrient transportersin mammalian cells. The purpose of the study was to investigate the influence of intracellular glutamine status onamino acid transport system A in placental choriocarcinoma cells (BeWo). Intracellular glutamine was depleted byculturing the cells in regular medium without glutamine and by treating the cells for 16 hours with methioninesulfoximine (MSX), an inhibitor of glutamine synthetase. When cultured in the presence of glutamine, treatment withMSX had no effect on system A activity as monitored by MeAIB uptake. However, when cultured in the absence ofglutamine, treatment with …
The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo), Boonrit Thongsong
The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo), Boonrit Thongsong
The Thai Journal of Veterinary Medicine
The intracellular concentration of glutamine is very high where it plays a central role as a ready source ofnitrogen, carbon and energy in various metabolic processes. The purpose of the present study was to investigate theinfluence of intracellular glutamine status on amino acid uptake activity in placental choriocarcinoma (BeWo) cells,analogous to normal placental trophoblasts. Intracellular glutamine was depleted by culturing the cells in regularmedium without glutamine and by treating with 2 mM methionine sulfoximine (MSX), an inhibitor of glutaminesynthetase, for 16 hours. The uptake of various amino acids was measured by the use of appropriate substrates andionic conditions. When cultured …