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Full-Text Articles in Medicine and Health Sciences
Effects Of Semen Extender On Motility And Movement Patterns Of Frozen-Thawed Eld’S Deer (Cervus Eldii) Spermatozoa, Sukumal Rittem, Daraka Thongthainun, Wanlaya Tipkantha, Boripat Siriaroonrat, Nikorn Thongtip
Effects Of Semen Extender On Motility And Movement Patterns Of Frozen-Thawed Eld’S Deer (Cervus Eldii) Spermatozoa, Sukumal Rittem, Daraka Thongthainun, Wanlaya Tipkantha, Boripat Siriaroonrat, Nikorn Thongtip
The Thai Journal of Veterinary Medicine
Two semen samples of Siamese Eld,s deer (Cervus eldii siamensis: SED) and seven semen samples of Thamin Eld,s deer (Cervus eldii thamin: TED) were collected by using electroejaculation. Each ejaculate was diluted and cryopreserved with 2 extenders; 1) TRIS; and 2) modified BF5F supplemented with 5% final glycerol concentration. The movement patterns of frozen-thawed spermatozoa were assessed using computer-assisted sperm analysis (CASA). In TED, the results revealed a significantly higher (p< 0.05) percentage of total motility (%TM), percentage of progressive motility (%PM), track speed (VCL) and lateral amplitude (ALH) in TRIS extender. The results also revealed a significantly low (p< 0.05) progressive velocity (VSL), straightness (STR) and linearity (LIN) in TRIS extender. However, for path velocity (VAP) and beat frequency (BCF), there were no differences between both extenders. In SED, sperm motility and sperm movement parameters did not show differences between TRIS and modified BF5F extenders. Findings from this study will aid in Eld’s deer semen banking and artificial insemination success in the future.
Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The aims of the present study were to determine the number of spermatozoa in the reproductive tract ofsows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence ofadding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteenmultiparous sows were divided into 3 groups: group I (n= 5), insemination with 80 ml of extended fresh semen,group II (n= 4), insemination with 40 ml of FT semen diluted with ModenaTM extender and group III (n= 5),insemination with 40 ml of FT semen diluted with combination of ModenaTM and seminal plasma. …