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Detection Of Enzootic Bovine Leukosis In Cattle Using Nested Polymerase Chain Reaction Assay, Jomelson Alfaro Uera, Jonathan Ventura Lazaro, Claro Niegos Mingala
Detection Of Enzootic Bovine Leukosis In Cattle Using Nested Polymerase Chain Reaction Assay, Jomelson Alfaro Uera, Jonathan Ventura Lazaro, Claro Niegos Mingala
The Thai Journal of Veterinary Medicine
Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV) infection. BLV was detected incattle using nested polymerase chain reaction (PCR) assay and were identified BLV infected cattle farms in fiveselected provinces in the Philippines. A total of 300 cattle blood samples were used. BLV Proviral DNA wasextracted and amplified using nested PCR assay targeting the BLV long terminal repeat (LTR). Results showed that11 samples (3.67%) of the 300 cattle blood samples used were positive for BLV infection. This study is considered firstreport of cattle EBL in the Philippines.
Seroprevalence And Risk Factors Associated With Leptospirosis (L. Interrogans) In Bovine Cattle In Northeastern Mexico, Verónica Carvajal-De La Fuente, Cecilia Zapata-Campos, Jorge Loredo-Osti, Rigoberto López-Zavala, José Osiel Jasso-Obregón, Edgar Martínez-Bautista
Seroprevalence And Risk Factors Associated With Leptospirosis (L. Interrogans) In Bovine Cattle In Northeastern Mexico, Verónica Carvajal-De La Fuente, Cecilia Zapata-Campos, Jorge Loredo-Osti, Rigoberto López-Zavala, José Osiel Jasso-Obregón, Edgar Martínez-Bautista
The Thai Journal of Veterinary Medicine
The seroprevalence of Leptospira interrogans serovars in cattle was determined by analyzing 385 serum samples from 8 Rural Development Districts (RDD), in Tamaulipas, Mexico. A microscopic agglutination test (MAT) including 9 serovars of L. interrogans as antigens was used. Serum samples were considered positive when 50% or more of agglutination in a dilution of ≥ l:100 was observed. A total of 70.4% animals were reported positive for one or more serovars. The most frequent antibodies reported were those against serovars tarassovi (53.25%) hardjo (23.64%) and canicola (15.32%), whilst serovar bataviae (0.52%) was found at lower frequency. Mante district showed the …