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Swine High Fever Syndrome: What Prrsv Gotta Do With It?, Roongroje Thanawongnuwech Dec 2010

Swine High Fever Syndrome: What Prrsv Gotta Do With It?, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

No abstract provided.


Effects Of Dietary Melamine Or Urea-Formaldehyde Or Their Mixtures On Performance, Carcass Quality, Melamine Residues And Microscopic Changes In Broiler Tissues, Srisuda Sirilaophaisan, Jowaman Khajarern, Bundit Tengjarernkul Dec 2010

Effects Of Dietary Melamine Or Urea-Formaldehyde Or Their Mixtures On Performance, Carcass Quality, Melamine Residues And Microscopic Changes In Broiler Tissues, Srisuda Sirilaophaisan, Jowaman Khajarern, Bundit Tengjarernkul

The Thai Journal of Veterinary Medicine

A study was conducted to determine the effects of dietary graded levels of melamine, urea-formaldehyde (UF) and their mixture on growth performance, carcass quality, melamine residues and microscopic changes in broiler tissues from d-1 to d-42 followed by a 7-d feeding of withdrawal diets. One thousand and forty 1-d-old equal mixed-sex Arber Acres broiler were assigned to 13 dietary treatments with 4 replicate pens of 20 chicks assigned to each treatment. The diets of melamine, UF and their mixture contained four graded levels (0.25, 0.50, 0.75 and 1.00%) but no melamine or UF was added to the control diet. There …


Molecular Classification Of Infectious Laryngotracheitis Virus From Chick Embryo Origin Vaccine, Tissue Culture Origin Vaccine And Field Isolates, Wisanu Wanasawaeng, Niwat Chansiripornchai Dec 2010

Molecular Classification Of Infectious Laryngotracheitis Virus From Chick Embryo Origin Vaccine, Tissue Culture Origin Vaccine And Field Isolates, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The molecular classification of the infectious laryngotracheitis virus from chicken embryo origin (CEO) vaccine, tissue culture origin (TCO) vaccine and field isolates by the sequence of ICP4 genes from CEO and TCO vaccines in the GenBank was performed for the different sequence analysis. The sequences of TCO vaccine virus (accession no. EU104908) were used as a prototype for a primer design to cover the region of 450 basepairs (bp) by Primer3 software. The simulation sequences of CEO vaccine virus (accession no. EU104900) and TCO vaccine virus (accession no. EU104908) from PCR reaction were selected for restriction enzyme. Hga I could …


Salbutamol Residues In Swine Tissues And Body Fluids After Feeding, Jen Hung Ming, Hsiang Huang Han, Ling Chen Chao, Jen Wu Yu, Katie Marie Dixon, Liang Mao Chi Dec 2010

Salbutamol Residues In Swine Tissues And Body Fluids After Feeding, Jen Hung Ming, Hsiang Huang Han, Ling Chen Chao, Jen Wu Yu, Katie Marie Dixon, Liang Mao Chi

The Thai Journal of Veterinary Medicine

Salbutamol residues in swine tissues and fluids were investigated. Tissue, serum and urine samples were collected from 33 pigs after 14 days of oral administration of 3 ppm salbutamol. The concentrations of salbutamol were determined by enzyme-linked immunosorbent assay and further confirmed by capillary electrophoresis. The highest urinary concentration of salbutamol was 145.12 ng/ml at 18 hour and remained detectable for 30 days after the last feeding. Salbutamol was undetectable in serum samples. The highest concentrations of salbutamol in liver, kidney, lung, heart, brain and muscle were 70.42 ng/g, 31.88 ng/g, 26.06 ng/g, 6.76 ng/g, 3.41 ng/g and 2.97 ng/g, …


Comparative Study Of Temperatures Used In Silicone Impregnation Of Porcine Hearts Plastination, Damri Darawiroj, Adisorn Adirekthaworn, Sayamon Srisuwattanasakul, Kongkiat Srisuwattanasakul Dec 2010

Comparative Study Of Temperatures Used In Silicone Impregnation Of Porcine Hearts Plastination, Damri Darawiroj, Adisorn Adirekthaworn, Sayamon Srisuwattanasakul, Kongkiat Srisuwattanasakul

The Thai Journal of Veterinary Medicine

Forced impregnation process is normally performed under vacuum at freezing temperature which requires expensive plastination apparatus. To reduce the cost in the plastination process, forced impregnation of porcine hearts under two different environments, which are at the freezing temperature (-20oC) and at room temperature (22-25oC), was compared. Twelve porcine hearts were collected from a slaughter house, and divided into the groups, six in each, and subjected to be plastinated by S10 standard technique at the two temperatures. Although the forced impregnation at room temperature, also the curing process, was more time-consuming, all hearts from both groups produced satisfying plastinated specimens. …


An Outbreak Of Avibacterium Paragallinarum Serovar B In A Thai Layer Farm, Kridda Chukiatsiri, Suwit Chotinun, Niwat Chansiripornchai Dec 2010

An Outbreak Of Avibacterium Paragallinarum Serovar B In A Thai Layer Farm, Kridda Chukiatsiri, Suwit Chotinun, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Avibacterium paragallinarum serovar B was diagnosed and revealed in a layer farm. The infected chickens showed mucous nasal discharge, facial edema and conjunctivitis. Egg production also decreased dramatically. Necropsy findings showed lesions of conjunctivitis and edematous tissue around the eyes. The infraorbital sinuses were filled with a caseopurulent mass and tracheitis. Bacterial isolation and identification was performed and confirmed by PCR technic. A hemagglutination inhibition test using specific antibodies against serovar B revealed the Avibacterium paragallinarum serovar B infection. A pathogenicity test done in 10 layer chickens, showed clinical signs of nasal discharge, facial edema and conjunctivitis. This is the …


Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn Dec 2010

Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk Dec 2010

Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aim of the present study is to determine the distribution of immune cells within the endosalpinx related to the ovarian function in gilts culled due to repeat breeding and anoestrus. A total of 30 reproductive organs from culling crossbred Landrace x Yorkshire gilts were collected from commercial swine herds in Thailand and categorized into 3 groups, i.e. anoestrus, repeat breeding and non-involved in reproductive disorder gilts (control group). The ovaries and oviducts were separated from the reproductive tracts and macroscopically investigated. The oviducts were divided into the utero-tubal junction (UTJ), the isthmus, the ampulla and the infundibulum. The gilts …


Tetranucleotide Microsatellite Markers For Molecular Testing In Thai Domestic Elephants (Elephas Maximus Indicus), Duangsmorn Suwattana, Jutarat Jirasupphachok, Sumolya Kanchanapangka, Weerapong Koykul Dec 2010

Tetranucleotide Microsatellite Markers For Molecular Testing In Thai Domestic Elephants (Elephas Maximus Indicus), Duangsmorn Suwattana, Jutarat Jirasupphachok, Sumolya Kanchanapangka, Weerapong Koykul

The Thai Journal of Veterinary Medicine

Eleven microsatellite loci were evaluated for genetic profiles of Thai domestic elephants (Elephas maximus indicus) and their suitability as genetic markers for molecular testing. A total of 66 animals were tested. 10 out of 11 loci could be amplified and they demonstrated polymorphism with allelic numbers per locus ranging from 7 (LaT06) to 39 (LaT18). Values of expected heterozygosity (He) and Polymorphic Information Content (PIC) were between 0.6449 (LaT17) – 0.9593 (LaT05) and 0.5934 (LaT17) – 0.9578 (LaT05), respectively. Analysis of the ten microsatellite markers revealed Combined Exclusion Probability (CEP) of 99.99998783% or 1.2167 x 10-7 and 99.91% confident for …


The Efficacy Of Pure Natural Repellents On Rat Responses Using Circular Open Field, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul Dec 2010

The Efficacy Of Pure Natural Repellents On Rat Responses Using Circular Open Field, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

Rodent infestation is considered as one of the major pest problems since it is not only acting as a health hazard, but also causes damages to household, agricultural fields and even transportation business. The use of rodenticides is a common approach to rodent control, however, most rodenticides are poisonous to man. Therefore, using natural extracts as a rat repellent may be a better alternative. In the current study, various natural extracts were experimented whether they could repel a rat when testing in the behavioral model, a circular open field. The tested substances were wintergreen oil, chilli, peppermint oil, bergamot oil …


In Vitro Testing Of The Efficacy Of Organic Releasing Chlorine (Virusnip™) Against Escherichia Coli, Salmonella Spp. Candida Albicans And Trichophyton Mentagophytes, Indhira Kramomtong, Waree Niyomtham, Sasiwimon Talummuk, Penchan Chaiyanate, Kai Sievert Dec 2010

In Vitro Testing Of The Efficacy Of Organic Releasing Chlorine (Virusnip™) Against Escherichia Coli, Salmonella Spp. Candida Albicans And Trichophyton Mentagophytes, Indhira Kramomtong, Waree Niyomtham, Sasiwimon Talummuk, Penchan Chaiyanate, Kai Sievert

The Thai Journal of Veterinary Medicine

The antimicrobial activities of organic releasing chlorine disinfectant (Virusnip™) was evaluated and compared by semi-quantitative suspension-neutralization method under clean condition in vitro. The test organisms were clinical bacterial isolates of hemolytic Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Hadar, Salmonella Infantis, and Salmonella Virchow. Fungal isolates were Candida albicans and Trichophyton mentagophytes. Four sets of Virusnip™ concentrations were prepared at 1:100, 1:200, 1:400 and 1:1000 for bacteria and 3 sets of 1:100, 1:200 and 1:400 for fungi. Each set of various concentrations was kept at room temperature for 1, 2 and 7 days before use. The assays were performed at …


Comparative Study Between Using Acetone And Absolute Alcohol For Dehydration In Plastination Procedure, Kongkiat Srisuwatanasagul, Sayamon Srisuwatanasagul, Adisorn Adirekthaworn, Damri Darawiroj Dec 2010

Comparative Study Between Using Acetone And Absolute Alcohol For Dehydration In Plastination Procedure, Kongkiat Srisuwatanasagul, Sayamon Srisuwatanasagul, Adisorn Adirekthaworn, Damri Darawiroj

The Thai Journal of Veterinary Medicine

In order to develop the most practical plastination specimens used for anatomical study, the aim of the present study was to compare between using acetone and absolute alcohol for dehydration in plastination procedure. Ten pig heart specimens were used in the present study and plastination procedure was processed by using acetone (group 1) or absolute alcohol (group 2) for dehydration. The results showed that dehydration by acetone resulted in more natural color with no shrinkage of the heart specimens while dehydration by alcohol caused darker color and more shrinkage. However, the advantage of using alcohol was that it gave the …


Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai Dec 2010

Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai

The Thai Journal of Veterinary Medicine

Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A …


Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat Dec 2010

Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

K. parviflora is a medicinal plant possessing high potential for development of various health products. The objective of this chronic toxicity study was to investigate the safety of ethanolic extract of chronic Kaempferia parviflora Wall ex Bak in Wistar rats. The animals were randomly divided into five groups, twenty four rats each (12 males and 12 females). Three treatment groups were orally administered with K. parviflora extract at doses of 5, 50 and 500 mg/kg/day for six months respectively, which were equivalent to 1, 10 and 100 times of human use, while two control groups were orally given with distilled …


Ophthalmology Snapshot, Nalinee Tuntivanich Dec 2010

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph Dec 2010

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2010

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Cost-Effectiveness Analysis Of Alkaline Lysis, Magna Pure, And Phenol-Chloroform Dna Extraction Methods Followed By Measurement Of Single Gene Copy Number Using Quantitative Real-Time Pcr For Dirofilaria Immitis Microfilaria, S Sungpradit, S Nuchprayoon, T. Chatsuwan Nov 2010

Cost-Effectiveness Analysis Of Alkaline Lysis, Magna Pure, And Phenol-Chloroform Dna Extraction Methods Followed By Measurement Of Single Gene Copy Number Using Quantitative Real-Time Pcr For Dirofilaria Immitis Microfilaria, S Sungpradit, S Nuchprayoon, T. Chatsuwan

Chulalongkorn Medical Journal

Objective : Dirofilariasis or heartworm disease, a mosquito-borne parasitic disease, continues to be a serious problem in canine (dogs) and feline (cats) species. The disease is caused mainly by the filarial nematode, Dirofilaria immitis. In canine dirofilariasis, the adult worms, present in the pulmonary arteries and the right ventricle, causing damages and leading to pulmonary hypertension and right-sided congestive heart failure. In addition, acute death in feline dirofilariasis causes lung injury resulting in respiratory distress. Being critical to transmission and complex disease pathology, the microfilarial stage is useful in laboratory diagnosis; however, the microfilaria identification process is laborious, difficult and …


Wolbachia Of Arthropods And Filarial Nematodes: Biology And Applications, S Sungpradit, S. Nuchprayoon Nov 2010

Wolbachia Of Arthropods And Filarial Nematodes: Biology And Applications, S Sungpradit, S. Nuchprayoon

Chulalongkorn Medical Journal

Wolbachia is a group of obligatory intracellular gram-negative bacteria. This bacterium is responsible for manipulation of host reproduction in many arthropod species and involved in embryonic and larval development, adult female fertility, and survival in filarial nematode. The applied biology of Wolbachia and modification of pest and vector species using Wolbachia have been studied in order to suppress or modify natural populations. In addition, Wolbachia has been used as a novel chemotherapeutic target for filariasis control program. This article reviews the biology of Wolbachia in arthropods and filarial nematodes, including recent advance and future directions of using Wolbachia as a …


Survey Of Knowledge Of Outpatients On Locations In The Refrigerator For Eye Drop Keeping, S Pornvichitpisan, N. Laohapojanart Nov 2010

Survey Of Knowledge Of Outpatients On Locations In The Refrigerator For Eye Drop Keeping, S Pornvichitpisan, N. Laohapojanart

Chulalongkorn Medical Journal

Some kinds of eye drop such as Latanoprost, Chloramphenicol, Cefazolin, Ceftazidime, Vancomycin and Amphotericin B need to be kept at the temperature of 2-8 degree celsius to maintain their efficiency. Patients are usually recommended to keep these drugs in the refrigerator while dispensing. However, the temperature in the refrigerator is not consistent especially the refrigerator's door has higher temperature than other parts.


ย่อวารสาร, อลิสา จันทร์ปี, สุรางค์ นุชประยูร Nov 2010

ย่อวารสาร, อลิสา จันทร์ปี, สุรางค์ นุชประยูร

Chulalongkorn Medical Journal

No abstract provided.


ดรรชนีบทความจุฬาลงกรณ์เวชสาร 201, N/A Nov 2010

ดรรชนีบทความจุฬาลงกรณ์เวชสาร 201, N/A

Chulalongkorn Medical Journal

No abstract provided.


Ct Findings Of Pancreatic Ductal Adenocarcinoma At King Chulalongkorn Memorial Hospital: A Study Of 40 Cases With Histological Verification, E Swangjitmetta, L Vajragupta, N Wisedopas, N. Tanpowpong Nov 2010

Ct Findings Of Pancreatic Ductal Adenocarcinoma At King Chulalongkorn Memorial Hospital: A Study Of 40 Cases With Histological Verification, E Swangjitmetta, L Vajragupta, N Wisedopas, N. Tanpowpong

Chulalongkorn Medical Journal

Background : The incidence of pancreatic cancer has been increasing. CT scan is effective and standard modality for imaging of pancreatic cancer. Objective : To describe CT findings in pancreatic ductal adenocarcinoma at King Chulalongkorn Memorial Hospital and to subgroup analyze the enhancement patterns and presence of metastasis of each histological grading. Setting : Department of Radiology and Department of Pathology, Faculty of Medicine, Chulalongkorn University Design : Retrospective descriptive study Materials and Methods : Preoperative dual phase abdominal CT scans and pathological reports of 40 patients with pancreatic adenocarcinoma in King Chulalongkorn Memorial Hospital from 2003 to 2008 were …


Molecular Identification Of Maggots Captured From Forensic Cases, K Preativatanyou, N Eakachaiworrawut, P Chutivongse, N Sirisup, P. Siriyasatien Nov 2010

Molecular Identification Of Maggots Captured From Forensic Cases, K Preativatanyou, N Eakachaiworrawut, P Chutivongse, N Sirisup, P. Siriyasatien

Chulalongkorn Medical Journal

The estimation of the postmortem interval (PMI) is necessary for supporting justice to the dead. During the early stage of death, estimation of PMI of the corpses depends on the changing of muscle contraction and physical conditions, but this method can be used effectively in case of death within 24 hours. Flies are the first organisms which swarm on corpses. Consequently, accuracy of the estimation of PMI using fly larvae collected from corpses depends on identification of the species of flies. However, the macroscopic method using posterior spiracle is not definite for identifying the species of flies. Therefore, the molecular …


คำขอบคุณ, N/A Nov 2010

คำขอบคุณ, N/A

Chulalongkorn Medical Journal

No abstract provided.


ดรรชนีผู้แต่งจุฬาลงกรณ์เวชสาร 2010, N/A Nov 2010

ดรรชนีผู้แต่งจุฬาลงกรณ์เวชสาร 2010, N/A

Chulalongkorn Medical Journal

No abstract provided.


Canine Heartworm (Dirofilaria Immitis, Leidy) And Risk Of Infection In Human, S. Tiawsirisup Nov 2010

Canine Heartworm (Dirofilaria Immitis, Leidy) And Risk Of Infection In Human, S. Tiawsirisup

Chulalongkorn Medical Journal

Canine heartworm (Dirofilaria immitis) is a filarial nematode which dogs, cats and some wildlife creatures are definitive hosts, and humans are accidental hosts. Life cycle of this nematode involves the development in the mosquito vector. Microfilaria released from a female nematode can be found in the definitive host's blood circulation and is required to develop into the third or infective stage larva in the mosquito. Infective stage larva will be released from infected mosquito's mouthpart into the host's skin during the course of taking the blood meal, develop into an adult stage in the definitive host, and reside in the …


Wolbachia Isolation By Fractioned Centrifugation From Dirofilaria Immitis For Protein Analysis, V Sanprasert, S Sungpradit, S. Nuchprayoon Nov 2010

Wolbachia Isolation By Fractioned Centrifugation From Dirofilaria Immitis For Protein Analysis, V Sanprasert, S Sungpradit, S. Nuchprayoon

Chulalongkorn Medical Journal

Objective : The biological roles of Wolbachia, a filarial intracellular bacteria, have promoted applied researches in lymphatic filariasis. Analysis of Wolbachia proteins will provide complementary information on the biology of Wolbachia, as well as the immune mechanisms against filarial nematodes. We therefore developed a Wolbachia isolation method from Dirofilaria immitis (dog heartworm) for protein analysis. Methods : Wolbachia bacteria were homogenized and isolated by fractioned centrifugation by using 0.85% NaCl supplemented with varying concentration of Nonidet P-40 (NP-40) as the homogenization buffer. The purity of Wolbachia extracts were analyzed by sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE), and by quantitative polymerase …


A Case Report Of Severe Adverse Reactions Following Diethylcarbamazine Treatment Of Lymphatic Filariasis, S Nuchprayoon, V Sanprasert, P Jaratsing, S. Jaijakul Nov 2010

A Case Report Of Severe Adverse Reactions Following Diethylcarbamazine Treatment Of Lymphatic Filariasis, S Nuchprayoon, V Sanprasert, P Jaratsing, S. Jaijakul

Chulalongkorn Medical Journal

Diethylcarbamazine (DEC) treatment for lymphatic filariasis can be accompanied by adverse drug reactions. Severe adverse reactions are usually uncommon but once happen, they could be acute and sometimes life-threatening. We report here a case of 15-year-old patient who was treated with DEC and developed severe adverse reactions including fever, headache, dizziness, chest pain, sore throat, cough, dyspnea, nausea and vomiting. A complete profile of clinical findings and blood parameters, including parasitological, hematological, biochemical, and immunological parameters was reported. The physical examination showed rhonchi on chest examination. Laboratory investigations revealed leukocytosis with the increased levels of neutrophils and monocytes. The bimodal …


Active Management Of The Third Stage Of Labor (Amtsl) Without Controlled Cord Traction At Nongkai Hospital, S. Densiriaksorn Nov 2010

Active Management Of The Third Stage Of Labor (Amtsl) Without Controlled Cord Traction At Nongkai Hospital, S. Densiriaksorn

Chulalongkorn Medical Journal

Objective : To compare simplified active management of the third stage of labor (AMTSL) package with the full AMTSL package in the active management of the third stage of labor with respect to the amount of bleeding. Design : Randomized controlled trial. Setting : Nongkai Hospital. Materials and Methods : Two hundred women with imminent vaginal delivery were randomized into two groups, namely: the study and control groups in the study group, simplified package of the third-stagelabor management [oxytocin injection, cord clamping, uterine message, without controlled cord traction (CCT)] were performed. In the control group, full package of the third-stage-management …