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Full-Text Articles in Medicine and Health Sciences
Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs
Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs
The Thai Journal of Veterinary Medicine
Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations in freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify the fundamental differences between high (HF) and low freezability (LF) ejaculated spermatozoa in swamp buffaloes, which were limitedly described for this agricultural species. Twelve (12) different samples of bubaline semen were collected, cryopreserved and categorized into freezability phenotypes using post-thaw sperm motility as the basis. Sperm kinematics and functional plasma membrane integrity were evaluated in HF and LF samples using computer-assisted sperm analysis (CASA) and hypo-osmotic swelling test, respectively. The relative concentrations of some …
The Optimal Divalent Cations And Storage Temperatures For The Encapsulation Of Ram Spermatozoa, Pintira Thiangthientham, Junpen Suwimonteerabutr, Theerawat Tharasanit, Mongkol Techakumphu
The Optimal Divalent Cations And Storage Temperatures For The Encapsulation Of Ram Spermatozoa, Pintira Thiangthientham, Junpen Suwimonteerabutr, Theerawat Tharasanit, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the effects of encapsulation with different divalent cations and temperatures on the quality of ram spermatozoa during cold storage. Experiment 1: diluted semen was allocated randomly into three groups: control, encapsulation with calcium (Ca2+) alginate and encapsulation with barium (Ba2+) alginate. The samples were stored at two different temperatures (4 °C or 16 °C) for 24 h and subsequently examined for spermatozoa quality. Experiment 2: determination of sperm functionality by means of sperm binding was performed. Spermatozoa with and without Ba2+ alginate were cooled for 24 h and then used for sperm …
Comparison Of Different Methods For Sperm Vitality Assessment In Frozen-Thawed Holstein Bull Semen, Kakanang Buranaamnuay
Comparison Of Different Methods For Sperm Vitality Assessment In Frozen-Thawed Holstein Bull Semen, Kakanang Buranaamnuay
The Thai Journal of Veterinary Medicine
The present study evaluated the accuracy of four methods, i.e. eosin-nigrosin, trypan blue, hypo-osmotic swelling test (HOST) and Hoechst 33342 (H342)/propidium iodide (PI) for assessing bovine sperm vitality. Frozen-thawed semen (n = 30) of Holstein bulls was layeredon 40%/80% percoll solutions to isolate viable spermatozoa. An aliquot of viable spermatozoa was kept at 37°C (live sample); the rest was submitted to cold shock (dead sample). The two aliquots were mixed in three proportions, corresponding to 0%, 50% and100% of viable cells; the sperm vitality was analyzed. The percentages of viable spermatozoa evaluated with the four methods significantly correlated with the …
Evaluation Of Two Cryopreservation Protocols On Cauda Epididymal Spermatozoa Characteristics In Domestic Cats, Kanokwan Thammapradit, Suppawiwat Ponglowhapan
Evaluation Of Two Cryopreservation Protocols On Cauda Epididymal Spermatozoa Characteristics In Domestic Cats, Kanokwan Thammapradit, Suppawiwat Ponglowhapan
The Thai Journal of Veterinary Medicine
Various protocols of feline spermatozoa cryopreservation have been reported and used in clinical practice. To compare the quality of post-thawed epididymal spermatozoa subjected to two different sperm freezing protocols, sperm samples were collected from domestic cats (n=7) after castration and divided into 2 aliquots as for Protocol 1 (P1) or Protocol 2 (P2). In P1, after centrifugation, sperm pellets were extended with Tris-based egg yolk extender (TEY) containing 3% glycerol and held for 60 min (5°C) prior to the second dilution (1:1) with 7% glycerol and 1% Equex STM Paste. The straws were frozen by lowering the goblets in three …
Cryopreservation Effect On Expression Of Sex Steroid Receptors Of Boar Spermatozoa, Sukanya Manee-In, Panida Chanapiwat, Nawarus Prapaiwan, Kampon Kaeoket, Sayamon Srisuwatanasagul, Atthaporn Roongsitthichai
Cryopreservation Effect On Expression Of Sex Steroid Receptors Of Boar Spermatozoa, Sukanya Manee-In, Panida Chanapiwat, Nawarus Prapaiwan, Kampon Kaeoket, Sayamon Srisuwatanasagul, Atthaporn Roongsitthichai
The Thai Journal of Veterinary Medicine
This study aimed to investigate the expressions of estrogen receptor (ER) alpha, ER beta, and progesterone receptor (PR) during semen cryopreservation, to evaluate the correlation among ERa, ERβ, PR, capacitation, acrosome reaction, and semen quality parameters, and to investigate the effect of different concentrations of L-cysteine on the expressions of ERa, ERβ, and PR. Semen samples were collected from 12 boars [Duroc (n = 4), Landrace (n=4), and Large white (n=4)]. The controlled rate-freezing method was used to cryopreserve the semen samples. The samples were collected at different times: fresh semen, after adding ModenaTM, after adding extender II (TE2), after …
Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu
Effect Of Various Combinations Of Sugar Supplementation In The Extender On Frozen-Thawed Ram Semen Quality And Fertility, Saritvich Panyaboriban, Junpen Suwimonteerabutr, Nawapen Phutikanit, Theerawat Swangchan-Uthai, Theerawat Tharasanit, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Semen cryopreservation alters sperm structure and biochemical properties, and subsequently impairs fertilizing ability. A combination of two different sugar molecules revealed an enhanced beneficial effect on frozen-thawed spermatozoa of various species. However, the report in ram has been scarce. The present study investigated the effect of different combinations of sugar supplementation on post-thawed ram semen qualities in a cryopreservative extender in the presence of a single sugar or a combination of sugars. Data showed that a combination of sucrose and trehalose significantly improved cryopreserved sperm motility, viability, longevity and acrosome integrity. Fertility rates following laparoscopic artificial insemination (LAI) using frozen-thawed …
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in …
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Computer-assisted sperm analysis (CASA) was initiated to reduce subjective bias on the motility assessment and to discriminate a series of motility patterns of boar semen. The objectives of this present study were to evaluate the motility pattern of frozen-thawed boar semen at 0 and 60 min after thawing using CASA. Forty one ejaculates from Landrace (n=14), Yorkshire (n=12) and Duroc (n=15) boars were cryopreserved and included in the experiment. The semen was thawed at 37°C for 30 sec. The post-thawed sperm qualities including subjective motility, plasma membrane integrity and motility pattern were determined immediately after thawing (T0) and at 60 …
Dogs Spermatozoa Labelling With 99mtc-Hmpao, Kaywalee Chatdarong, Eva Axnér, Bernt Jones, Catharina Linde Forsberg
Dogs Spermatozoa Labelling With 99mtc-Hmpao, Kaywalee Chatdarong, Eva Axnér, Bernt Jones, Catharina Linde Forsberg
The Thai Journal of Veterinary Medicine
The study was aimed at finding out whether dog sperm could be labelled using 99mTc-HMPAO and finding the optimum incubation temperature and amount of 99mTc-HMPAO to label spermatozoa from fresh ejaculates and from frozen-thawed semen. A radiopharmaceutical was prepared by reconstituting 0.5 mg of HMPAO with 5 ml pertechnetate solution containing 100 kBq 99mTc. Semen samples were obtained from three dogs, pooled and divided into aliquots containing 100x106 spermatozoa each. Fresh and frozen-thawed semen samples were used. In experiment 1, the fresh and frozen-thawed spermatozoa were incubated with 99mTc-HMPAO at 20oC or 37oC for 20 min. In experiment 2, 0.01, …
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
The Thai Journal of Veterinary Medicine
During sperm transport in the female pig, a proportion of the spermatozoa is arrested at the uterotubal junction and the isthmus, where an oviductal sperm reservoir is built up. The sperm reservoir maintains sperm viability and controls the release of fertilizing spermatozoa so that only a small subpopulation reaches the site of fertilization, thus diminishing the risk of polyspermy. In vitro research has focused on sperm binding as the main mechanism of sperm storage, sperm release and modulation of capacitation, but little attention has been paid to the sperm reservoir fluid, its composition and the control of capacitation in vivo. …
The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai
The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai
The Thai Journal of Veterinary Medicine
In the pig, sperm transport is regulated, to facilitate normal fertilization of the ovulated oocytes, by the oviduct which contains a particular segment, the sperm reservoir. The main function of the sperm reservoir is to ensure that stored spermatozoa have maintained viability and fertilizing capacity. The process of sperm capacitation is delayed in the sperm reservoir, during the lengthy pre-ovulatory estrus by interactions between the spermatozoa, the oviduct epithelium and secretion products. Sperm capacitation seems to be facilitated by the tubal fluid in the upper segment of the oviduct releasing sperm from the reservoir in relation to the time of …