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Full-Text Articles in Medicine and Health Sciences
Chylomicrons Produced By Caco-2 Cells Contained Apob-48 With Diameter Of 80-200 Nm, Andromeda M. Nauli, Yuxi Sun, Judy D. Whittimore, Seif Atyia, Guha Krishnaswamy, Surya M. Nauli
Chylomicrons Produced By Caco-2 Cells Contained Apob-48 With Diameter Of 80-200 Nm, Andromeda M. Nauli, Yuxi Sun, Judy D. Whittimore, Seif Atyia, Guha Krishnaswamy, Surya M. Nauli
Pharmacy Faculty Articles and Research
The small intestine generally transports dietary fats to circulation in triglyceride (TG)-rich lipoproteins. The two main intestinal lipoproteins are chylomicron (CM) and very low-density lipoprotein (VLDL). Unfortunately, studies on the CM biogenesis and intestinal transport of dietary fats have been hampered by the lack of an adequate in vitro model. In this study, we investigated the possible factors that might increase the efficiency of CM production by Caco-2 cells. We utilized sequential NaCl gradient ultracentrifugation to isolate the CMs that were secreted by the Caco-2 cells. To confirm the successful isolation of the CMs, we performed Fat Red 7B staining, …