Medicine and Health Sciences Commons^™

Bile Acid Signal-Induced Phosphorylation Of Small Heterodimer Partner By Protein Kinase Cζ Is Critical For Epigenomic Regulation Of Liver Metabolic Genes, Sunmi Seok, Deepthi Kanamaluru, Zhen Xiao, Daniel Ryerson, Sung-E Choi, Kelly Suino-Powell, H. Eric Xu, Timothy D. Veenstra, Jongsook Kim Kemper

Pharmaceutical Sciences Faculty Publications

Bile acids (BAs) are recently recognized key signaling molecules that control integrative metabolism and energy expenditure. BAs activate multiple signaling pathways, including those of nuclear receptors, primarily farnesoid X receptor (FXR), membrane BA receptors, and FXR-induced FGF19 to regulate the fed-state metabolism. Small heterodimer partner (SHP) has been implicated as a key mediator of these BA signaling pathways by recruitment of chromatin modifying proteins, but the key question of how SHP transduces BA signaling into repressive histone modifications at liver metabolic genes remains unknown. Here we show that protein kinase Cζ (PKCζ) is activated by BA or FGF19 and phosphorylates …

Dsg3 As A Biomarker For The Ultrasensitive Detection Of Cccult Lymph Node Metastasis In Oral Cancer Using Nanostructured Immunoarrays, Vyomesh Patel, Daniel Martin, Ruchika Malhotra, Christina A. Marsh, Colleen L. Doçi, Timothy D. Veenstra, Cherie-Ann O. Nathan, Uttam K. Sinha, Bhuvanesh Singh, Alfredo A. Molinolo, James F. Rusling, J. Silvio Gutkind

Pharmaceutical Sciences Faculty Publications

OBJECTIVES: The diagnosis of cervical lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) patients constitutes an essential requirement for clinical staging and treatment selection. However, clinical assessment by physical examination and different imaging modalities, as well as by histological examination of routine lymph node cryosections can miss micrometastases, while false positives may lead to unnecessary elective lymph node neck resections. Here, we explored the feasibility of developing a sensitive assay system for desmoglein 3 (DSG3) as a predictive biomarker for lymph node metastasis in HNSCC.

MATERIALS AND METHODS: DSG3 expression was determined in multiple general cancer- and …