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Full-Text Articles in Medicine and Health Sciences
A Transforming Met Mutation Discovered In Non-Small Cell Lung Cancer Using Microarray-Based Resequencing, Torstein Tengs
A Transforming Met Mutation Discovered In Non-Small Cell Lung Cancer Using Microarray-Based Resequencing, Torstein Tengs
Dr. Torstein Tengs
We have designed resequencing microarrays to test the performance of this platform when interrogating a large number of exons (164 total) from genes associated with cancer. To evaluate false positive and negative rates, dideoxy sequencing was done for 335,420 bases interrogated by the arrays. From the array data, calls could be made forw97.5% of the bases, and false positive rates were very low with only a single mutation reported from the array dataset for which the corresponding dideoxy trace had a clean wildtype sequence. For the nucleotide positions where array calls were made, false negative rates were 1.41% for heterozygous …
Sensitive Mutation Detection In Heterogeneous Cancer Specimens By Massively Parallel Picoliter Reactor Sequencing, Torstein Tengs
Sensitive Mutation Detection In Heterogeneous Cancer Specimens By Massively Parallel Picoliter Reactor Sequencing, Torstein Tengs
Dr. Torstein Tengs
The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and by genetic heterogeneity within the cancer. Here, we show that microreactor-based pyrosequencing can detect rare cancer-associated sequence variations by independent and parallel sampling of multiple representatives of a given DNA fragment. This technology can thereby facilitate accurate molecular diagnosis of heterogeneous cancer specimens and enable patient selection for targeted cancer therapies.