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Articles 1 - 11 of 11
Full-Text Articles in Medicine and Health Sciences
Trophectoderm Differentiation In The Bovine Embryo: Characterization Of A Polarized Epithelium., L C Barcroft, A Hay-Schmidt, A Caveney, E Gilfoyle, E W Overstrom, P Hyttel, A J Watson
Trophectoderm Differentiation In The Bovine Embryo: Characterization Of A Polarized Epithelium., L C Barcroft, A Hay-Schmidt, A Caveney, E Gilfoyle, E W Overstrom, P Hyttel, A J Watson
Obstetrics & Gynaecology Publications
Blastocytst formation is dependent on the differentiation of a transporting epithelium, the trophectoderm, which is coordinated by the embryonic expression and cell adhesive properties of E-cadherin. The trophectoderm shares differentiative characteristics with all epithelial tissues, including E-cadherin-mediated cell adhesion, tight junction formation, and polarized distribution of intramembrane proteins, including the Na-K ATPase. The present study was conducted to characterize the mRNA expression and distribution of polypeptides encoding E-cadherin, beta-catenin, and the tight junction associated protein, zonula occludens protein 1, in pre-attachment bovine embryos, in vitro. Immunocytochemistry and gene specific reverse transcription--polymerase chain reaction methods were used. Transcripts for E-cadherin and …
Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz
Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz
Obstetrics & Gynaecology Publications
In the present study the abundance of mRNAs for eukaryotic translation initiation factors eIF-1A (formerly known as eIF-4C), -2alpha, -4A, -4E, and -5 was examined in in vivo-derived mouse embryos throughout preimplantation development using a semiquantitative reverse transcription-polymerase chain reaction assay. Although the mRNA profile for each gene is unique, only mRNA for eIF-1A transiently increases during embryonic gene activation (EGA) at the 2-cell stage, and this was confirmed by an independent hybridization-based assay. In in vitro-developed bovine embryos, mRNA for eIF-1A was transiently detected at the 8-cell stage, when the major activation of the genome occurs in this species. …
A Field Evaluation Of Ivomec-F For The Treatment Of Naturally Acquired Fasciola Gigantica And Other Gastrointestinal Nematode Infections In Cattle And Buffaloes, Tasanee Chompoochan, Suwannee Nithiuthai, Piyanoot Prasittirat
A Field Evaluation Of Ivomec-F For The Treatment Of Naturally Acquired Fasciola Gigantica And Other Gastrointestinal Nematode Infections In Cattle And Buffaloes, Tasanee Chompoochan, Suwannee Nithiuthai, Piyanoot Prasittirat
The Thai Journal of Veterinary Medicine
The efficacy of Ivomec-F was evaluated in 60 cattle and 14 buffaloes that had a naturally acquired infection of Fasciola gigantica. Some animals were also concurrently infected with gastrointestinal nematodes. The animals were divided equally into treatment and control groups. Treatment group 1 which consisted of 30 cattle, were administered a single subcutaneous dose of Ivomec-F (1% ivermectin /10% clorsulon) 1 ml/ 50 kg body weight. Control group 2 which consisted of 30 untreated cattle, were given the same volume of physiological saline. Groups 3 and 4 (7 treated and 7 control buffaloes) were treated in a similar manner as …
Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson
Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson
Obstetrics & Gynaecology Publications
This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-subunit polypeptides were detected by immunofluorescence to encircle the entire cell margins of each blastomere of inseminated zygotes, cleavage stage embryos, and morulae. Immunofluorescent localization of alpha1-subunit polypeptide in bovine blastocysts revealed an alpha1 immunofluorescence signal confined to the basolateral membrane margins of the trophectoderm and encircling the cell periphery of each inner cell mass (ICM) cell. In contrast, alpha …
Glycerol With Fetal Calf Serum - A Better Cryoprotectant For Blastocystis Hominis, Init Ithoi
Glycerol With Fetal Calf Serum - A Better Cryoprotectant For Blastocystis Hominis, Init Ithoi
Init Ithoi
In recent years, a growing number of reports have sug- gested that the protozoan parasite, Blastocystis hominis (Brumpt 1912), is a pathogenic cause of diarrhoea. It is imperative, however, that more studies are conducted on the various human isolates of the parasite before in- criminating it is a pathogen. In our laboratory we maintain human and animal isolates of Blastocystis using the monophasic medium Iscove's Modi®ed Dul- becco Medium (IMDM) as established by Ho et al. (1993), but maintaining continuous cultures is tedious, time consuming and expensive, and the parasites may be prone to genetic drift. There is therefore a …
Overexpression Of Glyoxalase-I In Bovine Endothelial Cells Inhibits Intracellular Advanced Glycation Endproduct Formation And Prevents Hyperglycemia-Induced Increases In Macromolecular Endocytosis., Moritsugu Shinohara, P J. Thornalley, Ida Giardino, Paul Beisswenger, Suzanne R. Thorpe, Joelle Onorato, Michael Brownlee
Overexpression Of Glyoxalase-I In Bovine Endothelial Cells Inhibits Intracellular Advanced Glycation Endproduct Formation And Prevents Hyperglycemia-Induced Increases In Macromolecular Endocytosis., Moritsugu Shinohara, P J. Thornalley, Ida Giardino, Paul Beisswenger, Suzanne R. Thorpe, Joelle Onorato, Michael Brownlee
Dartmouth Scholarship
Methylglyoxal (MG), a dicarbonyl compound produced by the fragmentation of triose phosphates, forms advanced glycation endproducts (AGEs) in vitro. Glyoxalase-I catalyzes the conversion of MG to S-D-lactoylglutathione, which in turn is converted to D-lactate by glyoxalase-II. To evaluate directly the effect of glyoxalase-I activity on intracellular AGE formation, GM7373 endothelial cells that stably express human glyoxalase-I were generated. Glyoxalase-I activity in these cells was increased 28-fold compared to neo-transfected control cells (21.80+/-0.1 vs. 0. 76+/-0.02 micromol/min/mg protein, n = 3, P < 0.001). In neo-transfected cells, 30 mM glucose incubation increased MG and D-lactate concentration approximately twofold above 5 MM (35.5+/-5.8 vs. 19.6+/-1.6, P < 0.02, n = 3, and 21.0+/-1.3 vs. 10.0+/-1.2 pmol/ 10(6) cells, n = 3, P < 0.001, respectively). In contrast, in glyoxalase-I-transfected cells, 30 mM glucose incubation did not increase MG concentration at all, while increasing the enzymatic product D-lactate by > 10-fold (18.9+/-3.2 vs. 18.4+/- 5.8, n = 3, P = NS, and 107.1+/-9.0 vs. 9.4+/-0 pmol/10(6) cells, n = …
Development Of A Blocking Enzyme-Linked Immunosorbent Assay For Detection Of Serum Antibodies To O157 Antigen Of Escherichia Coli., W Laegreid, M Hoffman, J Keen, R Elder, J Kwang
Development Of A Blocking Enzyme-Linked Immunosorbent Assay For Detection Of Serum Antibodies To O157 Antigen Of Escherichia Coli., W Laegreid, M Hoffman, J Keen, R Elder, J Kwang
Manuscripts, Articles, Book Chapters and Other Papers
The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica 09, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with E. coli O157:H7 LPS as the antigen and a monoclonal antibody specific for E. coli O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera …
Prevalence Of Mix Infectionsof Crytosporidium Spp. Eschricia Coli K99 And Rotavirus In The Faeces Of Diarrhoeic And Healty Cattle In Ankara..., Zişan Emre, Hatice Fi̇danci
Prevalence Of Mix Infectionsof Crytosporidium Spp. Eschricia Coli K99 And Rotavirus In The Faeces Of Diarrhoeic And Healty Cattle In Ankara..., Zişan Emre, Hatice Fi̇danci
Turkish Journal of Veterinary & Animal Sciences
Faecai specimens from 172 diarrhoeic and 130 healthy cattle were examined for the presence of Crytosporidium spp., Eschricia coli K99 and Rotavirus in Ankara, Turkey. The prevalence of the Crytosporidium spp. infection in diarrhoeic and non-dierrhoeic cattle was 63.3% and 69.2%, respectively. Crytosporidium positive faecal specimens were examined for the presence of E. coli K99 and Rotavirus. E. coli K99 was isolated from the feaces of 35 diarrhoeic (32.1%) and 23 healthy (25.5%) animals. Rotavirus was not detected in any of feacal samples. In vitro drug sensitivities indicated that E. coli K99 was sensitive to Nalidixic acid in both diarrhoeic …
Prevalence Of Cryptosporidium Spp. Infection And Its Relation To Other Entericpathogens (Escherichia Coli K 99 And Rotavirus) In Cattle In Ankara, Turkey, Zisan Emre, B. Metin Alabay, Hatice Fi̇danci, Ali Düzgün, Harun Çerçi̇
Prevalence Of Cryptosporidium Spp. Infection And Its Relation To Other Entericpathogens (Escherichia Coli K 99 And Rotavirus) In Cattle In Ankara, Turkey, Zisan Emre, B. Metin Alabay, Hatice Fi̇danci, Ali Düzgün, Harun Çerçi̇
Turkish Journal of Veterinary & Animal Sciences
Faecal samples of 172 diarrhoeic and 130 non-diarrhoeic cattle were examined for the presence of Cryptosporidium oocysts. The prevalence of infection in diarrhoeic and non-diarrhoeic cattle was 63.3% and 69.2%, respectively. The infection was prevalent in cattle over three years old, in both diarrhoeic and non-diarrhoeic animals. In both groups, oocyst sheeding level of (+) was the most common. Cryptosporidium-positive faecal specimens were examined for the presence of Escherichia coli K 99 and rotavirus. E. coli K 99 was isolated from the faeces of 35 (32.1%) diarrhoeic and 23 (25.5%) non-diarrhoeic animals. Rotavirus was not detected in any of the …
Eosinophilic Myositis In A Cattle, Ayhan Atasever
Eosinophilic Myositis In A Cattle, Ayhan Atasever
Turkish Journal of Veterinary & Animal Sciences
Eosinophilic myositis was described in the M. longissimus lumborum, M. latissimus dorsi, M. gluteus muscles of Holstein bull aged 18 month-old. Diffuse lesions were seen grossly. They were well-demarked, in green colour and 2-3 mm in diameter. Diffuse eosinophilic myositis were diagnosed microscopically.
Prevalence Of Bovine Eimeridosis In Thracia, Turkey, M. Özkan Arslan, Erkut Tüzer
Prevalence Of Bovine Eimeridosis In Thracia, Turkey, M. Özkan Arslan, Erkut Tüzer
Turkish Journal of Veterinary & Animal Sciences
In this study, 768 faecal samples were examined between June 1993-May 1994 to determine the species and the prevalence rates of Eimeriidae in the cattle of Thracia area (The European part of Turkey). The prevalence rate of eimeridosis was found to be 68%. The species determined and their prevalence rates were as follows: Eimeria bovis(34%), E.auburnensis(27%), E.zeuernii(26%), E.ellipsoiDalıs (14%), E.canadensis (12%), E.cylindrica (7.9%), E.supspherica (7.2%), E.alabamensis (4.9%), E.bukidnonensis (2.2%), E.brasiliensis (0.8%) and Isospora sp. (1.2%). Of 768 animals, 29% were found to be infected with single species, 39% with mixed ones and 32% were non-infected. During the study, two calves …