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Full-Text Articles in Medicine and Health Sciences
Progesterone Inhibits Apoptosis In Part By Pgrmc1-Regulated Gene Expression, John J. Peluso, Xiufang Liu, A Gawkowska, Valentina Lodde, Carol A. Wu
Progesterone Inhibits Apoptosis In Part By Pgrmc1-Regulated Gene Expression, John J. Peluso, Xiufang Liu, A Gawkowska, Valentina Lodde, Carol A. Wu
UCHC Articles - Research
Progesterone Receptor Membrane Component-1 (PGRMC1) is present in both the cytoplasm and nucleus of spontaneously immortalized granulosa cells (SIGCs). PGRMC1 is detected as a monomer in the cytoplasm and a DTT-resistant PGRMC1 dimer in the nucleus. Transfected PGRMC1-GFP localizes mainly to the cytoplasm and does not form a DTT-resistant dimer. Moreover, forced expression of PGRMC1-GFP increases the sensitivity of the SIGCs to progesterone (P4) 's anti-apoptotic action, indicating that the PGRMC1 monomer is functional. However, when endogenous PGRMC1 is depleted by siRNA treatment and replaced with PGRMC1-GFP, P4 responsiveness is not enhanced, although overall levels of PGRMC1 are increased. P4's …