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Full-Text Articles in Medicine and Health Sciences
Immunohistochemical Detection Of Feline Viral Rhinotracheitis In A Cat, Nopadon Pirarat, Yumi Une, Yasuo Nomura
Immunohistochemical Detection Of Feline Viral Rhinotracheitis In A Cat, Nopadon Pirarat, Yumi Une, Yasuo Nomura
The Thai Journal of Veterinary Medicine
A female, 10-month old, mixed breed cat showed clinical signs of severe dehydration, purulent nasal discharge, dyspnea, severe crepitating lung sounds and no response to antibiotic treatment. Upon necropsy, histopathology revealed severe, diffuse, fibrinopurulent pleuropneumonia and numerous, homogeneous, amphophilic, intranuclear inclusion bodies in the pulmonary alveolar macrophages, bronchial and alveolar epithelium and bronchial glandular epitheliums. Immunohistochemical studies using 3 different monoclonal, anti-Feline Herpesvirus type 1 antibodies, revealed immunoreactivity of viral antigen in the lung that was associated with the histopathological changes. Monoclonal, anti-glycoprotein B, antibody showed the strongest immunoreactivity.
The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura
The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura
The Thai Journal of Veterinary Medicine
Formalin fixed tissues from an aborted foal which had been diagnosed as having Equine Herpesvirus (EHV) infection, on histopathological criteria, were examined for EHV antigen using immunohistochemistry. EHV antigen was found in the focal necrotic area of the liver, especially in hepatocytes and Kupffer cells. Intranuclear and intracytoplasmic, immuno-positive labelings were also prominent in bronchiolar epithelial cells. The method allowed the visualization of the antigenic site distribution and their subsequent correlation with histopathological changes.