Medicine and Health Sciences Commons^™

Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens

Electronic Theses and Dissertations

In the United States, osteoporosis results in about 1.5 million annual fractures, costing approximately $15 billion. Calcitonin is safe and effective in slowing osteoporotic bone loss, but its effect is transient. The current studies were designed to explore the dose-dependent effects of salmon calcitonin on bone turnover in ovariectomized rats and to determine if the decrease in therapeutic effectiveness of calcitonin demonstrated over time with higher doses is due to oversuppression of bone turnover. Doses of 5, 15, & 50 IU/kg BW/day of calcitonin were compared to placebo in 12-week-old ovariectomized and sham-ovariectomized Sprague-Dawley rats for 24 weeks. The spinal …

Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers

Electronic Theses and Dissertations

Azithromycin, a macrolide antibiotic, specifically binds to the 50S ribosomal subunit of bacterial ribosomes and inhibits translation. Azithromycin also prevents 50S ribosomal subunit assembly by binding to a 50S ribosomal subunit precursor particle. When exposed to azithromycin, several ribonucleases in wild-type Escherichia coli cells degrade antibiotic-bound 50S precursor particles. Presumably, cells expressing one or more mutated ribonucleases will degrade the antibiotic-bound precursor less efficiently, resulting in increased sensitivity to the antibiotic. To test this, eight ribonucleaseûdeficient strains of Escherichia coli were grown in the presence or absence of azithromycin. Cell viability, growth rates, and protein synthesis rates were measured. Degradation …

Role Of Macrophage Apoptosis In Atherosclerosis., June Liu

Electronic Theses and Dissertations

The presence of apoptotic cells in atherosclerotic lesions has been broadly reported in the past ten years. The majority of these apoptotic cells are macrophages. However, the pathogenic role of macrophage apoptosis in the development of atherosclerosis remains to be elucidated. Elevated expression of Bax, one of the pivotal pro-apoptotic proteins of the Bcl-2 family, has been found in human atherosclerotic plaques. Activation of Bax also occurs in free cholesterol-loaded and oxysterol treated mouse macrophages. In this study, we evaluated the influence of Bax deficiency on apoptosis in macrophage-like P388D1 cells by using small interfering RNA (siRNA) to suppress Bax …

Childhood Experiences Of Appalachian Women Who Have Experienced Intimate Partner Violence During Adulthood., Amy L. Reeves

Electronic Theses and Dissertations

Recent national surveys in the United States estimate one in five females will experience abuse by an intimate partner during her lifetime. Previous quantitative research linked childhood victimization to repeated victimization in adult relationships. This qualitative study explored the lived experiences of childhood in eight female victims of intimate partner violence who were born and reared in southern Appalachia. Interviews were analyzed using a descriptive-interpretative phenomenological method, as described by Van Manen. The three essential themes from childhood were identified as: living 'as if' an orphan; surviving in chaos; and, manifesting a devalued self. These themes were congruent with findings …

Papain: A Novel Urine Adulterant., David Burrows

Electronic Theses and Dissertations

The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false negative result on commonly used primary drug screening methods such as the Fluorescence Polarized Immunoassay (FPIA) technique. Typical chemical adulterants such as nitrites are easily detected or render the urine specimen invalid as defined in the proposed federal guidelines for specimen validity testing based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The …

Early Speech And Language Development: A Comparison Of Typically Developing Children To Children With Cleft Palate., Holly Jannice Mcgahey

Electronic Theses and Dissertations

Previous research has shown that parent implemented intervention is effective in increasing the speech and language development of children with cleft lip and palate. To further determine the efficacy of this intervention, this study compared the speech and language development of children with CLP, who received parent implemented intervention, with a group of younger, typically developing children, matched for vocabulary size. This study also evaluated the language differences between the mothers of both groups of children. Speech and language assessments were administered to the typically developing children and their mothers at two times to mirror the time of assessment for …

Nongenomic Effects Of Estrogens On Epithelial Chloride Secretion., Sabyasachi Moulik

Electronic Theses and Dissertations

The human colonic cell line T84, a model for studying epithelial chloride secretion and cystic fibrosis chloride channel (CFTR) function, was used to investigate the regulatory role of estrogens in transepithelial ion transport. Estrogens and other steroid hormones do not stimulate chloride secretion by themselves. However, 17 β-estradiol (17β-E2) rapidly (within seconds to minutes) potentiates carbachol- and thapsigargin-stimulated chloride secretion measured as short circuit current in voltage-clamped T84 monolayer cultures. The cholinergic agonist carbachol and the SR Ca²⁺ ATPase inhibitor thapsigargin stimulate chloride secretion by elevating intracellular calcium. 17α-estradiol, a stereoisomer that does not activate nuclear estrogen receptors, is …

The Effects Of A Multimodality Approach On Sentence Production Using Response Elaboration Training With A Reading Component On Aphasic Patients., Sara E. Mccarthy

Electronic Theses and Dissertations

The effects of a multimodality treatment were investigated using a single subject experimental design across behaviors in two patients with different severity levels of Broca's aphasia. We hypothesized that Response Elaboration Training and an oral reading task would improve accuracy of sentence production, information content, and mean length of utterance. Results indicated that this treatment approach elicited significant improvement in the accuracy of sentence production and information content in the participant with very mild Broca's aphasia. Furthermore, the participant with severe Broca's aphasia demonstrated a very significant improvement in information content and mean length of utterance. The improvements support the …

An Enhanced Dialogic Reading Approach To Facilitate Typically Developing Pre-School Children's Emergent Literacy Skills., Sheri E. Davis

Electronic Theses and Dissertations

This study investigated an enhanced dialogic reading (DR) approach in facilitating emergent literacy skills in typically developing preschool children. Eight children from a Title One preschool and their parents participated in five weekly 90-minute training sessions that focused on phonological awareness, print awareness, and alphabet knowledge. First order effects were examined in parent questioning and interaction behaviors on pre- and post-training videotapes. Second order effects were examined in the children's outcomes from pre- to posttesting of preliteracy, speech, and language skills. Results indicated that parents made significant increases in their initiations and responses and a significant decrease in their Mean …

The Role Of Osteopontin In Vascular Remodeling, Daniel Myers

Electronic Theses and Dissertations

Chronic obstructive vascular disease is a major contributor to mortality in developed nations. The pathology typically involves neointima formation accompanied by vascular remodeling in the presence of inflammation. The disease can be modeled in animals with the response to a direct injury of the vasculature. This text describes a project examining the role of osteopontin (OPN) in vascular remodeling. The projectis goal was to mechanistically describe the contribution of OPN to the vascular injury response. The hypothesis that the presence of OPN affects the formation of neointima and constrictive remodeling associated with vascular injury was addressed using the strategy of …

Contexts For Facilitating Emergent Literacy In Typically Developing Preschoolers., Karen Nicole Wilhjelm

Electronic Theses and Dissertations

The purpose of this study was to investigate if there is an additive benefit in training Emergent Literacy (EL) skills with typically developing preschoolers using a combined intervention approach, Dialogic Reading (DR) plus classroom Phonological Awareness (CL), than a single intervention approach providing only DR training.

The study consisted of 8 preschoolers (5 DR+CL, 3 DR). The classroom PA training was conducted 3 times a week for 5 weeks for 20 minutes, using play-based activities. The DR training programs were held once a week for 5 weeks for 90 minutes teaching strategies that could be used in the home environment. …

The Type And Frequency Of Morphosyntax Errors In Children's Narratives., Jennifer Lynn Lockhart

Electronic Theses and Dissertations

A subsample of 478 children who originally participated in a larger study (Tomblin et al., 1997) was examined for type and frequency of morphological noun and verb errors in oral and written narratives in 2^nd and 4^th grade. Each child represented one of four groups: Typical Language, Specific Language Impairment, Nonspecific Language Impairment, and Low Nonverbal IQ. Three MANOVA's and post-hoc comparisons were used to test three predictions: (1) children will have more difficulty with verb than noun morphology; (2) children will make more errors in the written than the spoken narratives; and (3) children whose language impairments …

The Effect Of Closed Versus More Liberal Visitation Policies On Work Satisfaction Beliefs And Nurse Retention., Suzanne M. Boswell

Electronic Theses and Dissertations

Nurses believe that more liberal visitation policies will cause greater work overload and decrease the quality of patient care. The purpose of this study was to investigate the effect of a liberal visitation policy on work satisfaction, beliefs, and retention of nurses.

In this study, nurses were asked to complete a survey before and after implementation of a more liberal visitation policy. The nurses were surveyed using Stamps (1997) Index of Work Satisfaction and the Boswell Beliefs Inventory. Statistical analyses were conducted to discover nurses level of satisfaction and beliefs related to the new policy.

Results indicate that nurses were …

Religion And Crime: A Study Of Inmates In State And Federal Prisons In The United States., Dhanalakshmi Thirumalai

Electronic Theses and Dissertations

Persistent high levels of crime in this country have provided support for the need for public programs that help in reducing crime rates through effective interventions. Spirituality and religion are considered to play a major part in determining an individual's attitude towards committing a crime. This study analyzed if inmates in State and Federal prisons in the US are involved in prison religious activities and personal prayers in order to determine if criminality (measured by offense type) of inmates affects their participation in religious activities. Based on logistic regression analysis, gender, race, and marital status of inmates were found to …

The Speech Situation Checklist: A Normative And Comparative Investigat, Susha Verghese

Electronic Theses and Dissertations

Studies conducted over the past decades have identified the presence of a greater amount of negative emotional reaction and speech disruption in particular speech situations among children who stutter, compared to those who do not (Brutten & Vanryckeghem, 2003b; Knudson, 1939; Meyers, 1986; Trotter, 1983). Laboratory investigations have been utilized to describe the particular situations that elicit the greatest or least amount of speech concern and fluency failures. More recently, in order to deal with the limitation of laboratory research, the use of self-report tests have gained popularity as a means of exploring the extent of negative emotional reaction and …

Rate Of Graduation Among Students With Histories Of Specific Language Impairment, Amy Jean Hadley

Electronic Theses and Dissertations

The rate of graduation among students with histories of specific language impairment (SLI) was investigating by comparing the target population to matched, nondisabled peers. Data regarding the graduation outcomes for 176 matched pairs of students, over a five-year period, were analyzed to determine if a significant difference in achievement of a high school diploma was observable between the two groups of students. In addition, the study addressed the variables of socioeconomic status and attendance rate as related to graduation rate. For students with histories of specific language impairment, model of service delivery was also examined with regard to graduation rate. …

Effects Of Simultaneous Exercise And Speech Tasks On The Perception Of, Heather Koblick

Electronic Theses and Dissertations

The purpose of this study was to investigate the effects of voice production and perception of dyspnea in aerobic instructors during simultaneous tasks of exercise and speech production. The study aimed to document changes that occur during four conditions: 1) voice production without exercise and no use of amplification; 2) voice production without exercise and the use of amplification; 3) voice production during exercise without the use of amplification; 4) voice production during exercise with the use of amplification. Participants included ten aerobic instructors (two male and eight female). The dependent variables included vocal intensity, average fundamental frequency (F0), noise-to-harmonic …

Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya

Electronic Theses and Dissertations

Anthrax, a fatal bacterial infection is caused by Bacillus anthracis, a gram-positive, spore forming, capsulated, rod shaped organism. Centers for Disease Control (CDC) lists anthrax as Category A biological agent due to its severity of impact on human health, high mortality rate, acuteness of the disease and potential for delivery as a biological weapon. The currently available human vaccine in the United States (AVA anthrax vaccine adsorbed) is prepared from Alum adsorbed formalin treated supernatant culture of toxigenic, non-encapsulated strain of Bacillus anthracis with the principle component being protective antigen (PA83). Evaluation of anthrax vaccine given to nearly 400,000 US …

Estimation Of Standardized Mortality Ratio In Geographic Epidemiology, Anna Kettermann

Electronic Theses and Dissertations

The analysis of geographic variation of disease and its representation on a map form an important topic of research in epidemiology and in public health in general. Identification of spatial heterogeneity of relative risk using morbidity and mortality data is required. The usual technique of disease atlas generation consists of data collection (observed number of disease cases). These data are collected during a continuous period of time (5 to 10 years). The second aspect of atlas creation relates to the analysis of these data. A traditional measure of the spatial variation is usually taken as a ratio of the number …