Medicine and Health Sciences Commons^™

Characterization Of A 30s Ribsomal Subunit Intermediate Found In Escherichia Coli Cells Growing With Neomycin And Paromomycin., Cerrone Renee Foster

Electronic Theses and Dissertations

The bacterial ribosome is a target for inhibition by numerous antibiotics. Neomycin and paromomycin are aminoglycoside antibiotics that specifically stimulate the misreading of mRNA by binding to the decoding site of 16S rRNA in the 30S ribosomal subunit. Recent work has shown that both antibiotics also inhibit 30S subunit assembly in Escherichia coli and Staphylococcus aureus cells. This work describes the characteristics of an assembly intermediate produced in E.coli cells grown with neomycin or paromomycin. Antibiotic treatment stimulated the accumulation of a 30S assembly precursor with a sedimentation coefficient of 21S. The particle was able to bind radio labeled antibiotics …

Adolescents And Marijuana Use: The Affects Of Peer And Parent Relationships And Substance Abuse Education., Samuel Joseph Cosimano

Electronic Theses and Dissertations

The purpose of this study was to analyze gender, race, substance abuse programs such as Drug Abuse Resistance Education (D.A.R.E.), parents, and peers and their ability to influence or predict adolescents and their decisions to use marijuana. All of the variables used for this study came from secondhand data collected by Esbensen and Osgood (1999), Gang Resistance Education and Training (G.R.E.A.T.). The analysis revealed that males are more likely to have ever used marijuana, that mixed race adolescents have a higher rate than other races to have ever used marijuana, that when adolescents complete the substance abuse program, D.A.R.E. have …

The Effects Of Nicotine Conditioned Place Preference In D2 Primed Adolescent Rats: Age-Related And Gender Effects., Yoko Emily Ogawa

Electronic Theses and Dissertations

This study investigated nicotine conditioned place preference (CPP) in two different ages of adolescence using a rodent model of schizophrenia. Both 2- and 3-chambered CPP apparatuses were used to test whether the CPP was due to an aversion to the white chamber. Animals were neontally treated with the dopamine D₂/D₃ agonist, quinpirole, or saline and raised to either early postweanling age (P 22) or adolescence (P 29). Rats were conditioned to prefer the white chamber using nicotine. Results showed that nicotine induced CPP and appeared to alleviate an increased stress response in D₂ primed animals, which …

The Effect Of Caffeine And Ethanol On Flatworm Regeneration., Erica Leighanne Collins

Electronic Theses and Dissertations

Flatworms, or planarian, have a high potential for regeneration and have been used as a model to investigate regeneration and stem cell biology for over a century. Chemicals, temperature, and seasonal factors can influence planarian regeneration. Caffeine and ethanol are two widely used drugs and their effect on flatworm regeneration was evaluated in this experiment. Non-toxic levels of caffeine, a stimulant, and ethanol, a depressant, were determined. The tails of the flatworms were removed and the regeneration stage was analyzed every 3 days for 15 days to see the effect of these drugs alone and in combination on regeneration. For …

Comeback Of Appalachian Female Stroke Survivors: The Interrelationships Of Cognition, Function, Self-Concept, Interpersonal, And Social Relationships., Patricia Mckenzie Vanhook

Electronic Theses and Dissertations

The purpose of this descriptive correlational study was to examine the comeback phase of the Trajectory of Chronic Illness Management for Appalachian women stroke survivors. This study predicted comeback as a relationship between physical healing, biographical reengagement, and psychological coming to terms that was mediated by rehabilitation.

The concepts were evaluated through investigation of function and cognitive abilities, self-assessment of recovery, health rating, ability to control life, relationship changes, physical and mental health, and limitations of activities due to poor physical or mental health. Data were collected using, the Stroke Impact Scale (3.0) (Duncan, Bode, Lia, & Perera, 2003), the …

Wellness Program Effect On The Health Parameters Of Female Employees Aged 25 To 60 Years Of Age Targeting Physical Activity And Nutrition Therapy., Adrienne J. Poag

Electronic Theses and Dissertations

The purpose of the study was to determine if the health parameters of female employees would improve, as measured in the posttest data, following the completion of a 12-week employee wellness program. The participants included 17 female employees from the Carter County Health Department located in Elizabethton, Tennessee. The principle investigator assessed the changes in the pretests and posttests and analyzed the data using SPSS. The results showed on average an increase in fruit and vegetable consumption and in the number of days exercised per week as well as improved diastolic blood pressure and triglyceride levels. The employees who experienced …

Studies On A 50s Ribosomal Precursor Particle As A Substrate For Erm E Methyltransferase Enzyme In Staphylococcus Aureus ., Indira Pokkunuri

Electronic Theses and Dissertations

Erythromycin is a macrolide antibiotic that inhibits not only mRNA translation but also 50S ribosomal subunit assembly in bacterial cells. An important mechanism of erythromycin resistance is the methylation of 23S rRNA by erm methyl transferase enzymes. We are interested in investigating the true substrate for methylation because it is known from our work and the work of others that fully assembled 50S subunits are not substrates for methylation. We have published a model for 50S ribosomal subunit formation where, the precursor particle that accumulates in erythromycin treated cells is a target for methyl transferase activity. Current studies are aimed …

An Analysis Of Nicotine Exacerbation Of Reductions In Ppi In A Rodent Model Of Schizophrenia., Amanda Marie Maple

Electronic Theses and Dissertations

Prepulse inhibition (PPI) is an operational measure of sensorimotor gating and is known to be reduced when the dopamine D² receptor is activated. We used a rodent model of psychosis in which increases in dopamine D_{2 receptor sensitivity are produced through neonatal quinpirole (a dopamine D2 / D3 agonist) treatment to rats. Rats were administered quinpirole (1mg/kg) or saline from postnatal day (P) 1-21. Rats were raised to adulthood and tested on PPI. Results showed that neonatal quinpirole treatment produced a significant reduction in PPI, and nicotine exacerbated this reduction. This reduction was partially blocked by …}

Post Traumatic Stress Symptoms And Critical Incident Stress Debriefing (Cisd) In Emergency Medical Services (Ems) Personnel., Ginger Lee Woods

Electronic Theses and Dissertations

EMS personnel were examined for Post Traumatic Stress symptoms and the usefulness of Critical Incident Stress Debriefing (CISD) using the Los Angeles Symptom Checklist (LASC) and a demographics questionnaire. This study revealed that women in this group show higher PTSD symptoms than male coworkers. Level of Training (LOT) of the EMS provider did not demonstrate a significant difference in whether a provider developed PTSD. EMS personnel receiving debriefing actually suffered greater levels of PTSD than those that did not receive debriefing. And 16% of EMS providers in this study suffered from PTSD, while approximately 20% suffered from partial PTSD or …

Bronchioalveolar Carcinoma In Jefferson And Mccracken Counties, Kentucky: Gender Differences In Survival., Jasneet Aneja

Electronic Theses and Dissertations

Bronchioalveolar carcinoma (BAC), a rare lung cancer, is more common in women, has a high proportion of non-smokers, and better survival, especially in women, than other lung cancers. Study subjects were 83 BAC patients from two Kentucky counties. Mean survival differences were compared by selected variables. The results showed better survival for females (6.5 years) than males (3.0 years, p-value 0.02); for urban (4.3 years) compared to rural residents (2.6 years, p-value 0.04); and for females with history of hysterectomy (5.1 years) compared to females without such history (3.3 years, p-value 0.02); the last finding supports a hormonal role in …

Temperament And Early Communication In Premature Children., Nina King

Electronic Theses and Dissertations

Premature children are at greater risk for developing communication delays than full-term children, although it is not inevitable. Recent research links specific temperament characteristics to early language development. Thus, temperament may provide a way to identify premature children who are at increased risk. The first purpose of this study was to determine if temperament characteristics of premature children are the same as full-term children when measured by the Infant Behavior Questionnaire-Revised. The second purpose was to determine if temperament and language acquisition, as measured by the MacArthur Communicative Development Inventory: Words and Gestures, are linked in premature infants …

Nicotine Sensitization In A Rodent Model Of Schizophrenia: A Comparison Of Adolescents, Adults, And Neurotrophic Factors., Marla Kay Perna

Electronic Theses and Dissertations

The behavioral effects of nicotine on locomotor activity in a rodent model of psychosis were analyzed. This model is based on neonatal quinpriole treatment (a dopamine D2/D3 agonist) which causes increased D2 receptor sensitivity, a phenomenon known as D2 priming that is common in schizophrenia. D2-primed adolescent rats did not demonstrate nicotine-induced hypoactivity early in training, and males demonstrated more rapid sensitization to nicotine as compared to controls administered nicotine. D2-primed females administered nicotine demonstrated increased stereotypic behavior. D2-primed adult rats given nicotine demonstrated significantly more robust sensitization to nicotine than controls given nicotine. Brain-derived neurotrophic factor (BDNF) was analyzed …