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Full-Text Articles in Medicine and Health Sciences
Tuba, A Novel Protein Containing Bin/Amphiphysin/Rvs And Dbl Homology Domains, Links Dynamin To Regulation Of The Actin Cytoskeleton, Marco Antonio Del Valle Salazar
Tuba, A Novel Protein Containing Bin/Amphiphysin/Rvs And Dbl Homology Domains, Links Dynamin To Regulation Of The Actin Cytoskeleton, Marco Antonio Del Valle Salazar
Yale Medicine Thesis Digital Library
Tuba is a novel scaffold protein that functions to bring together dynamin with components of the actin cytoskeleton. It is concentrated at synapses in brain and binds dynamin selectively through four N-terminal Src homology-3 (SH3) domains. Tuba binds a variety of actin regulatory proteins, including N-WASP, CR16, WAVE1, WIRE, PIR121, NAP1, and Ena/VASP proteins, via a C-terminal SH3 domain. Direct binding partners include N-WASP and Ena/VASP proteins. Forced targeting of the C-terminal SH3 domain to the mitochondrial surface can promote accumulation of F-actin around mitochondria. A Dbl homology domain present in the middle of Tuba upstream of a Bin/amphiphysin/Rvs (BAR) …
Direct Evidence For A Membrane Deforming Motif In Endophilin: Implications Beyond Synaptic Vesicle Recycling, Khashayar Farsad
Direct Evidence For A Membrane Deforming Motif In Endophilin: Implications Beyond Synaptic Vesicle Recycling, Khashayar Farsad
Yale Medicine Thesis Digital Library
Effective neurotransmission is dependent on fast, reproduceable synaptic vesicle recycling. The synaptic vesicle recycling process is a complex event involving both protein-protein, as well as protein-lipid interactions. A central part of the retrieval process of synaptic vesicles lies in the ability of soluble proteins to deform the plasma membrane into a nascent bud which will eventually reform a fully competent synaptic vesicle upon fission. This process involves clathrin-coat proteins, which form a protein scaffold around the vesicle bud, as well as proteins which have more recently been thought to be involved in generating the high curvature membranes present at the …
Icam-1 Transmembrane Signaling: Involvement Of Taxreb107 In Icam-1-Mediated Signal Transduction, Brian Lee
Icam-1 Transmembrane Signaling: Involvement Of Taxreb107 In Icam-1-Mediated Signal Transduction, Brian Lee
Yale Medicine Thesis Digital Library
The role of membrane ICAM-1 in intercellular adhesion, through interaction with the leukocyte beta2 integrin receptors LFA-1 and Mac-1, is well established. However, until recently, ICAM-1 was widely regarded as being simply an "anchor" molecule, promoting, for example, firm adhesion of lymphocytes to vascular endothelial cells. The demonstration that ICAM-1 is connected to the actin-based cytoskeleton through interactions with subcortical actin-binding proteins, such as alpha-actinin, enhanced this concept. However, ICAM-1 has been increasingly shown to have signaling properties of its own. Engagement of ICAM-1 extracellularly, either through its natural counter-receptors, LFA-1/Mac-1, or through antibody mediated clustering, can produce numerous changes …
Nudel, An Unusual Mosaic Protease Involved In Defining The Embryonic Dorsal-Ventral Axis Of Drosophila Melanogaster, Charles Chansik Hong
Nudel, An Unusual Mosaic Protease Involved In Defining The Embryonic Dorsal-Ventral Axis Of Drosophila Melanogaster, Charles Chansik Hong
Yale Medicine Thesis Digital Library
Dorsal-ventral polarity of the Drosophila embryo is determined by positional information that originates outside of the embryo. This information, which is created during oogenesis by the somatic follicle cells swrounding the oocyte, is utilized following fertilization to trigger a spatially regulated serine protease cascade in the extraembryonic perivitelline space. This protease cascade, which appears to be activated only on the presumptive ventral side of the embryo, generates the ligand for the Toll transmembrane protein that polarizes the embryo by inducing ventral development.This thesis presents evidence that nudel, a gene maternally required in somatic tissue for the formation of Toll's ligand, …
Expression And Characterization Of Two Alternatively Spliced Isoforms Of Rat Type I Receptor For Inositol 1,4,5-Trisphosphate, Doris Da May Lin
Expression And Characterization Of Two Alternatively Spliced Isoforms Of Rat Type I Receptor For Inositol 1,4,5-Trisphosphate, Doris Da May Lin
Yale Medicine Thesis Digital Library
The transmembrane receptor for IP3 is a tetrameric calcium channel located in the endoplasmic reticulum. In many cell types the receptor mediates such complex signals as calcium waves and oscillations. There are multiple receptor isoforms, derived by alternative splicing as well as different genes. The functional significance of these many receptor isoforms has not been determined. To this end, I have generated cell lines that express homotetrameric receptors.Stable lines of the human embryonic kidney (HEK293) cells were established by transfection with cDNAs encoding for two splice variants of the rat type I receptor. The two variants differ in the presence …