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Full-Text Articles in Medicine and Health Sciences
Paclitaxel-Induced Apoptosis Is Bak-Dependent, But Bax And Bim-Independent In Breast Tumor, Anna V. Miller, Mark A. Hicks, Wataru Nakajima, Amanda C. Richardson, Jolene J. Windle, Hisashi Harada
Paclitaxel-Induced Apoptosis Is Bak-Dependent, But Bax And Bim-Independent In Breast Tumor, Anna V. Miller, Mark A. Hicks, Wataru Nakajima, Amanda C. Richardson, Jolene J. Windle, Hisashi Harada
Philips Institute for Oral Health Research Publications
Paclitaxel (Taxol)-induced cell death requires the intrinsic cell death pathway, but the specific participants and the precise mechanisms are poorly understood. Previous studies indicate that a BH3-only protein BIM (BCL-2 Interacting Mediator of cell death) plays a role in paclitaxel-induced apoptosis. We show here that BIM is dispensable in apoptosis with paclitaxel treatment using bim−/− MEFs (mouse embryonic fibroblasts), the bim−/− mouse breast tumor model, and shRNA-mediated down-regulation of BIM in human breast cancer cells. In contrast, both bak−/− MEFs and human breast cancer cells in which BAK was down-regulated by shRNA were more resistant to paclitaxel. However, paclitaxel …
Two-Component System Response Regulators Involved In Virulence Of Streptococcus Pneumoniae Tigr4 In Infective Endocarditis, My Trihn, Xiuchun Ge, Alleson Dobson, Todd Kitten, Cindy Munro, Ping Xu
Two-Component System Response Regulators Involved In Virulence Of Streptococcus Pneumoniae Tigr4 In Infective Endocarditis, My Trihn, Xiuchun Ge, Alleson Dobson, Todd Kitten, Cindy Munro, Ping Xu
Philips Institute for Oral Health Research Publications
Streptococci resident in the oral cavity have been linked to infective endocarditis (IE). While other viridans streptococci are commonly studied in relation to IE, less research has been focused on Streptococcus pneumoniae. We established for the first time an animal model of S. pneumoniae IE, and examined the virulence of the TIGR4 strain in this model. We hypothesized that two-component systems (TCS) may mediate S. pneumoniae TIGR4 strain virulence in IE and examined TCS response regulator (RR) mutants of TIGR4 in vivo with the IE model. Thirteen of the 14 RR protein genes were mutagenized, excluding only the essential …