Medicine and Health Sciences Commons^™

Sarcopenia And Aging: Skeletal Muscle Fiber Type Composition Of The Long-Lived Ames Dwarf Mouse Compared To Its Wild-Type Counterpart, Cory Leier

Physical Therapy Scholarly Projects

Skeletal muscle fiber type composition has not been studied in the long-lived Ames dwarf mouse when compared to its wild-type counterpart. Hindlimb soleus skeletal muscle from two 3-month old dwarf and two 3-month old wild-type mice was analyzed for muscle fiber size, proportion of type I (slow-twitch) and type II (fast-twitch) muscle fibers, myonuclei content per muscle fiber, and total cross sectional area of the muscle. Results revealed a significant difference in muscle fiber size (t(58)=-9.71, p

Muscle Fiber Type Characterization In The Ames Dwarf Mouse: A Model Of Delayed Aging, Angela M. Williams

Physical Therapy Scholarly Projects

Introduction: Normal muscle wasting due to aging, known as sarcopenia, has many implications in society, especially with a growing population of elderly people. In this study, muscle fiber size and count, nuclei count, and ratio of fast- to slow-twitch fibers was studied between Ames dwarf mice (df), an established model of aging, and wild-type mice (WT) to learn more about the mechanisms of aging, specifically related to skeletal muscle.

Methods: Four hind limb muscles were harvested from two Ames dwarf mice and two wildtype mice, sliced cross-sectionally and mounted on slides. After mounting, the slides were stained three different ways …