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Typing Of Methicillin-Resistant Staphylococcus Aureus By Ribosome Spacer And Arbitrarily Primed Polymerase Chain Reaction, Tamer Essawi, Kamel Adwan, Nael Abu-Hassan, Galeb Adwan, Ahmed Saleh, Ghaleb Mohammad Adwan
Typing Of Methicillin-Resistant Staphylococcus Aureus By Ribosome Spacer And Arbitrarily Primed Polymerase Chain Reaction, Tamer Essawi, Kamel Adwan, Nael Abu-Hassan, Galeb Adwan, Ahmed Saleh, Ghaleb Mohammad Adwan
Turkish Journal of Medical Sciences
Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RS-PCR) and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of AP-PCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.
Investigation Of The Bactericidal Effects Of Vancomycin And Quinupristin/Dalfopristin On Staphylococcus Aureus Isolates, Mi̇ne Hoşgör Li̇moncu, Şafak Ermertcan, Güner Coşar
Investigation Of The Bactericidal Effects Of Vancomycin And Quinupristin/Dalfopristin On Staphylococcus Aureus Isolates, Mi̇ne Hoşgör Li̇moncu, Şafak Ermertcan, Güner Coşar
Turkish Journal of Medical Sciences
The present study aimed to determine the correlation between the bactericidal activity of vancomycin and quinupristin/dalfopristin (Q/D) on Staphylococcus aureus isolates and their minimal inhibition concentrations. The in-vitro susceptibilities of the 99 S. aureus isolates to vancomycin and Q/D were investigated by agar dilution. Thirty methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA) vancomycin and Q/D susceptible isolates were involved in time-kill studies. While both MRSA and MSSA isolates were susceptible to vancomycin, 96% of both isolates were determined as susceptible to Q/D. In the time-kill test, after 6 h of incubation vancomycin exhibited a bactericidal activity of …