Medicine and Health Sciences Commons^™

Nanoparticle Toxicity And Molecular Mechanisms In Fish: A Case Study With Silver Nanoparticles, Jiejun Gao

Open Access Dissertations

Nanoparticles (NPs) are widely used in a myriad of commercial and industrial products making their entry to the environment a likely event. NPs have unique physical-chemical properties that result from their small size and high surface area to volume ratio, making them highly reactive and potentially toxic. In Chapter 1, we summarize the effects and mechanisms of metal-based NPs on the vascular system. In vitro studies have shown that NPs are anti-angiogenic because they cause inflammation, oxidative stress, and apoptosis of endothelial cells resulting in increased permeability and decreased proliferation and migration. Whole animal studies examining the effects of NPs …

The Development Of Preclinical Strategies For Facilitation Of Lead Candidate Selection, Christopher Dale Kulczar

Open Access Dissertations

Chapter 1 details a background of techniques used for modeling the blood-brain barrier (BBB). The BBB represents a diffusive barrier to both paracellular and transcellular movement of many compounds in and out of the brain. The main rate- limiting barriers of the BBB include exclusive tight junctions that prevent the movement of hydrophilic molecules through intercellular gaps, and efflux proteins in the membrane which pump many hydrophobic molecules back into the blood. In addition, the BBB contains metabolizing enzymes, including Cytochrome P450s. This barrier acts to protect the vulnerable tissues of the brain from harmful xenobiotics, but also can serve …

Ambient Ionization - Mass Spectrometry: Advances Toward Intrasurgical Cancer Detection, Alan Keith Jarmusch

Open Access Dissertations

My dissertation research has focused on the development of ambient ionization – mass spectrometry (MS) for clinical measurements, specifically intrasurgical cancer detection. The molecular differences between normal and cancerous tissue were detected via direct tissue analysis in vitro by touch spray ionization (TS) or by analyzing sectioned or smeared tissue using desorption electrospray ionization (DESI). The physical form of the tissue, e.g. in vitro sampling, sectioned, or smeared, was inconsequential in differentiating normal from cancerous tissue; however, the spectra acquired by TS and DESI differed due to differences in ionization processes. We envision that TS-MS and DESI-MS could impact diagnostic …

Examining The Impact Of The Affordable Care Act On Small Businesses And Industries In Northwest Indiana, Onias Muza Taruwinga

Open Access Dissertations

This research examined the lived experiences of small businesses in Northwest Indiana with the implementation of the Affordable Care Act. The interviews were conducted over a period of six months with an attempt to answer the question, “What was the impact of the Affordable Care Act on small businesses and industries in Northwest Indiana?” Additional research questions were, “Did the Affordable Care Act extend coverage to the uninsured, include other medical services that were not covered before, and reduce cost sharing and what lessons can be learned from the implementation of the Affordable Care Act for future policy implementation?” 15 …

Innovative Novel Immunotherapies For The Treatment Of Glioblastoma Multiforme, Salma Salem

Open Access Theses

Glioblastoma Multiforme GBM is a very aggressive type of malignant brain tumors that affects peoples’ lives. The diffusive, infiltrative, and metastatic behaviour of GBM is the major reason for the disease recurrence. The morphological and immunohistological characteristics of Central Nervous System (CNS) tumors including GBM are heterogeneous. GBM is either primary (de novo) or secondary to low-grade astrocytomas.

Current treatment options include surgery, radiotherapy, and temozolomide chemotherapy have not achieved any improvement in success rates over the past decades. The survival time reached by GBM patients was approximately 12 months only after being treated with radiotherapy alone without temozolomide. However, …

Exploring The Cause Of Injury Or Death In Grain Entrapment, Engulfment And Extrication, Salah F. Issa

Open Access Dissertations

Grain entrapments and engulfments are one of most common hazards associated with grain storage facilities. Since the 1970’s over 1,880 incidents have been documented in agricultural confined spaces of which 65% of all recorded incidents were grain entrapments and engulfments. There have been several studies conducted on the contributing factors behind these incidents; however, there have been very few attempts to understand the environmental, physiological or psychological factors the victims experience while entrapped, engulfed, or extricated. This includes understanding how secondary injuries are caused by grain or during extrication by first responders. The research effort was divided into three segments. …

Gonadal Intersex In Teleosts: Mechanisms, Molecular Biomarkers And Diagnostic Assays, Ahmed M.E. Abdel-Moneim Mohamed

Open Access Dissertations

Natural and synthetic estrogenic and androgenic compounds are continuously released into aquatic ecosystems. Exposure of teleost fishes to these contaminants can negatively impact sex differentiation and reproductive output. Specifically, development of gonadal intersex in gonochoristic (fixed sex) fish species has been studied extensively in relation to exposure to this class of compounds. The main objectives of this dissertation were to: 1) conduct field and laboratory studies to investigate the molecular signaling pathways behind the development of gonadal intersex; and 2) establish molecular biomarkers and assays for testing the ability of environmental pollutants to develop this condition using a battery of …

Optical Dosimetry Tools And Monte Carlo Based Methods For Applications In Image Guided Optical Therapy In The Brain, Akshay N Prabhu Verleker

Open Access Dissertations

Purpose: The long-term goal of this research is to determine the feasibility of using near infra-red light to stimulate drug release in metastatic lesions within the brain. In this work, we focused on developing the tools needed to quantify and verify photon fluence distribution in biological tissue. To accomplish this task, an optical dosimetry probe and Monte Carlo based simulation code were fabricated, calibrated and developed to predict light transport in heterogeneous tissue phantoms of the skull and brain. Empirical model (EM) of photon transport using CT images as input were devised to provide real-time calculations capable of being translated …

The Graphic Side Of Fear: The Effects Of Anti-Tobacco Graphic Threat Appeals, Latasha R. Swanson

Open Access Dissertations

Many public health organizations have proposed and implemented campaigns and policies involving graphic ads or warnings to deter individuals from smoking. The current study evaluated how high-cigarette dependent smokers evaluate these messages compared to low-cigarette dependent smokers using a mixed, 3 x 2 factorial design. A pilot study involving focus groups, interviews, and evaluation questionnaires (N=10) was conducted to classify graphic threat appeals and develop stimulus materials for the primary study. For the primary study, three graphic threat appeal conditions were evaluated by Amazon Mechanical Turk (MTurk) users (N=200). Findings suggested that the graphicness of the …

A Dose Distribution Study Of Uranyl Nitrate In Zebrafish Using Liquid Scintillation And Passivated Implanted Planar Silicon Detectors, Lee A. Alleman

Open Access Theses

Standard curves for a Perkin Elmer TriCarb 2800 liquid scintillation detector (LSC) and a Ludlum 3030p Passivated Implanted Planar Silicon detector have been developed and utilized for studying the dose distribution of depleted uranium (DU) within zebrafish. The DU source was crystallized uranyl nitrate (N2O8U•6H2O) solution, normally used for staining in electron microscopy with a manufactured average specific activity of 0.3 uCi/g. Zebrafish, both larvae and adults, were exposed to three different mass concentrations, dissected, dissolved and counted using an LSC. The counts were compared to the standard curve correlating the measured activity to that of the mass absorbed. It …

Design, Optimization, And Characterization Of Novel Polymer-Based Formulations For Controlled Release Of Drugs, Mario Alberto Cano Vega

Open Access Theses

Pharmaceutical products are a key aspect of treatment and prevention of disease. For example, dibenzazepine (DBZ) is a drug that has proved to be useful for the treatment of obesity while progesterone is a common drug for hormonal replacement therapy in women. However, administration of these drugs by conventional dosage forms offers little control over the drug distribution and concentration in the body and often result in unintended adverse consequences on other cells/tissues.

Recent advances in nanotechnology and polymer science have enabled the design and development of controlled release systems that would allow spatiotemporal delivery of drugs with improved efficacy. …

Manufacturer Perspectives On Content Transparency And Material Health In The Us Building Products Industry, Alexandra A. Muller

Open Access Theses

Flawed U.S. federal regulation of chemicals has resulted in a materials market that undervalues human and environmental health in favor of the more traditional attributes of price, performance and aesthetics. In the building products industry, global, dynamic supply chains and proprietary information concerns further complicate the task of assessing the material health of products.

Voluntary material health programs in the green building industry are intended to incentivize the manufacture and selection of safer products by getting companies to gather and assess ingredient, hazard and risk information from their supply chain. Building product manufacturers considered early adopters of the main material …

The Interactive Effects Of Pesticide Exposure And Infectious Disease On Amphibian Hosts, Katherine M. Pochini

Open Access Theses

Natural systems are home to a multitude of natural and anthropogenic stressors, which draw an array of effects on ecological communities. While these effects have been investigated individually, it is important, given the routine co-occurrence of these stressors, to understand their interactive effects. Pesticide exposure and infectious disease are two common, co-occurring stressors that each have documented detrimental effects on species and, as evidence suggests, may have interactive effects. Moreover, existing research suggests that these interactive effects are highly context dependent, eliciting different results based on species, disease agent, toxin, and environment. Given the variability with which species may experience …

A Study Of Applications Of 3d Animation For Emergency Medicine Pedagogy, Huilong Zheng

Open Access Theses

There is ample precedent for the use of 3D animation in education, though little research on specific applications in the field of medicine. An experiment was carried out to assess the suitability of 3D animation for educational purposes in emergency medicine courses. Two groups of experimental participants were assessed on their ability to respond to three emergency medical situations in simulated test scenarios. Both groups received equivalent information on how to treat the three different medical emergencies. The control group received the information in traditional lecture format. The experimental group instead received the information from a set of 3D animations. …

Data Mining Twitter For Cancer, Diabetes, And Asthma Insights, Kimberly Chulis

Open Access Dissertations

Twitter may be a data resource to support healthcare research. Literature is still limited related to the potential of Twitter data as it relates to healthcare. The purpose of this study was to contrast the processes by which a large collection of unstructured disease-related tweets could be converted into structured data to be further analyzed. This was done with the objective of gaining insights into the content and behavioral patterns associated with disease-specific communications on Twitter. Twelve months of Twitter data related to cancer, diabetes, and asthma were collected to form a baseline dataset containing over 34 million tweets. As …

Influence Of Dietary Component Manipulation And Feed Management Strategies On Growth And Rumen Development Of Weaned Dairy Heifers, Tana Shea Dennis

Open Access Dissertations

Well-developed replacement heifers provide a central foundation for the continued success of the dairy industry. Emphasis on improving pre-weaned calf nutrition has predominated in the industry, but opportunities exist to improve post-weaning heifer nutrition and management. We aimed to evaluate common feed management strategies seen in the industry and their effects on growth, feed efficiency (G:F), and rumen development of calves from birth to 8 mo of age using pen- and individually-fed animal trials. Little information exists regarding post-weaning performance and rumen development of calves fed conventional or high planes of nutrition pre-weaning; therefore, we evaluated two milk replacer feeding …

South Asian Women's Sexual Relationship Power: Examining The Role Of Sexism, Cultural Values Conflict, Discrimination, And Social Support, Chandni D. Shah

Open Access Dissertations

The lack of literature examining sexual experiences of South Asian women in dating relationships has important implications for the healthy development of long lasting romantic relationships. It is important to understand South Asian women’s relationship experiences in the context of power and sexism (interpersonal power framework; Pulerwitz, Gortmaker, & DeJong, 2000) and the role of specific sociopolitical factors (e.g., discrimination). Understanding South Asian women’s experiences of power in a sociopolitical context will help professionals when working with them to develop healthier sexual relationships through therapy outreach, and community programming. I used a correlational, quantitative study to examine the associations between …

Ultrasound And Photoacoustic Methods For Anatomic And Functional Imaging In Image Guided Radiation Therapy, Justin T. Sick

Open Access Dissertations

(MATERIAL and METHODS) First, we define the physical principals and optimal protocols that provide contrast when imaging with US and the transducer properties contributing to resolution limits. The US field of view (FOV) was characterized to determine the optimal settings with regard to imaging depth, focal region, with and without harmonic imaging, and artifact identification. This will allow us to determine the minimum errors expected when registering multimodal volumes (CT, US, CBCT).

Next, we designed an in-house integrated US manipulator and platform to relate CT, 3D-US and linear accelerator coordinate systems. To validate our platform, an agar-based phantom with measured …

Risks And Health Consequences Of Forgoing, Delaying, Or Having Trouble Accessing Needed Health Care Among Medicare Beneficiaries, Angelitta M. Spells

Open Access Dissertations

Medicare provides health care coverage for approximately 93% of non-institutionalized older adults. Compared to uninsured adults, Medicare beneficiaries have greater access to needed healthcare including preventative care. However, disparities in accessing needed health care still exist among Medicare beneficiaries. Prior research has described barriers to accessing needed health care among older Medicare beneficiaries, such as transportation and health system characteristics, but little is known about prevalence, risks, or health consequences of older Medicare beneficiaries delaying, forgoing, or having trouble accessing needed health care. The three studies included in this dissertation followed a nationally representative sample of older Medicare beneficiaries to …

Hydration And Vocal Loading On Voice Measures, Anusha Sundarrajan

Open Access Dissertations

Vocal loading adversely affects the healthy larynx. The negative effects of vocal loading are thought to be exacerbated in dry environments, noisy environments, using non-habitual speaking patterns, and voice quality. Advancing age is also thought to be a risk factor for the negative effects of loading. To systematically tease out the effects of these factors on the healthy larynx, three different experiments were conducted. In each experiment, healthy participants produced 45-minutes of child-directed speech. In experiment 1, older, healthy adults produced loud child-directed speech, in the presence of background noise, in both low and moderate humidities, and voice was assessed. …

Modeling Picking On Pharmaceutical Tablets, Shrikant Swaminathan

Open Access Dissertations

Tablets are the most popular solid dosage form in the pharmaceutical industry because they are cheap to manufacture, chemically and mechanically stable and easy to transport and fairly easy to control dosage. Pharmaceutical tableting operations have been around for decades however the process is still not well understood. One of the common problems faced during the production of pharmaceutical tablets by powder compaction is sticking of powder to the punch face, This is known as 'sticking'. A more specialized case of sticking is picking when the powder is pulled away form the compact in the vicinity of debossed features. In …

Evaluating The Prevalence And Effectiveness Of Breed-Specific Legislation, Felicia E. Trembath

Open Access Dissertations

Dog bites pose a persistent public health problem, which some jurisdictions pass breed-specific legislation (BSL) to address. However, very little non-anecdotal evidence regarding the efficacy of BSL has been presented. Currently, BSL research is hampered by the absence of standard terminology, an established prevalence, or a scientific consensus on its effectiveness. The purpose of this study is to propose standardized terminology for BSL, establish the prevalence of each type of BSL in the USA, and conduct a systematic review of the effectiveness of BSL.

After review of terminology currently in use, as well as review of the regulatory actions of …

Impact Of Peer Relationship And Exposure To Violence On Posttraumatic Stress For Children At Risk For Maltreatment, Aura Ankita Mishra

Open Access Theses

Post-traumatic Stress (PTS) symptomology includes ruminating thoughts and feelings around trauma, inability to feel and express emotions, and avoidance of things related to the traumatic event (American Psychiatric Association, 2000). Children and youth exposed to child maltreatment (abuse and neglect) are at higher risk of experiencing PTS. Extra-familial support, including peer support can reduce post-traumatic stress among youth (Pina et al., 2008). In the present study, Witnessing, Victimization, and Both Witnessing and Victimization due to Exposure to In-Home Violence and Peer Relationship Quality are evaluated as to their relative impact on PTS for children at risk for child maltreatment. Peer …

Immune Modulating Functions By Soypeptide Lunasin In Cancer Immunotherapy, Chun-Yu Tung

Open Access Dissertations

Chemotherapy is currently the mainstay of treatment for most cancer patients. Despite its efficacy in eliminating cancer cells, a high percentage of chemotherapy patients eventually relapse or suffer progression of the disease. Immunosurveillance is capable of recognizing and eliminating continuously arising transformed mutant cells, and thus cancer immunotherapy is one of the emerging therapeutic strategies that harnesses the power of the immune system to eradicate chemotherapy-resistant cancerous cells. However, the adverse side effects of chemotherapy impede the therapeutic effects of immunotherapy. Our previous studies demonstrate that lymphoma patients are refractory to clinical immunotherapy because of chemotherapy-induced immune dysfunction. In addition, …

Investigating The Role Of The Basic Helix-Loop-Helix Transcription Factor Mist1 In Pancreatic Diseases, Anju Karki

Open Access Dissertations

Acinar cells of the exocrine pancreas are dedicated to synthesize, package and secrete immense quantities of pro-digestive enzymes to maintain proper metabolic homeostasis for the organism. Dysregulation of enzyme secretion in acinar cells can give rise to exocrine diseases including acute pancreatitis (AP), a disease that targets acinar cells, leading to acinar-ductal metaplasia (ADM), inflammation and fibrosis—events that can transition into the earliest stages of pancreatic ductal adenocarcinoma (PDAC). The focus of this thesis is to interrogate transcriptional regulatory networks that are susceptible to AP and the role that these networks play in acinar cell and exocrine pancreas responses. The …

Influence Of The 3d Microenvironment On Glioblastoma Migration And Drug Response, Ruth Marisol Herrera Perez

Open Access Dissertations

Glioblastoma (GBM) is a highly invasive brain cancer characterized by poor prognosis. Despite significant efforts by the basic and clinical research community our understanding of GBM progression and recurrence has been incremental. Improvements in therapeutic response have been dismal, and GBM continues to be the deadliest tumor of the central nervous system, with patient average survival rate of 12 months. Synergistic relationships that the tumor cells establish with the brain microenvironment have been proven fundamental for successful tumor progression and maintenance. Yet, many in vitro GBM studies are performed in formats that fail to recapitulate the most essential component of …

Deficits In Sound Pattern Sequencing In Children With Specific Language Impairment: A Networks Approach, Sara J. Benham

Open Access Theses

Children with specific language impairment (SLI) demonstrate primary deficits in morphosyntax, which has served as the central theme in theoretical and clinical approaches. However, a striking number of children with SLI also exhibit speech sound deficits, characterized both by increased error patterns and by high levels of variability. These speech sound deficits have been under-studied and are not explicitly tied to accounts of SLI. In the present study, theoretical approaches drawn from dynamical systems and sequence learning are used to address speech production learning in children with SLI. Standard approaches to sound accuracy and variability and articulatory variability are integrated …

Mhealth To Improve The Diet Among Low-Income Populations Enrolled In An Established U.S. Nutrition Program: Design And Rationale Of A Randomized Controlled Trial, Lukas T. Ingersoll

Open Access Theses

Aim: To describe the theoretical basis, and implementation of a randomized controlled trial to improve the diet and nutrition of low-income families in Indiana.

Background: Changing difficult behaviors requires more than education. Beyond education, people benefit from counseling that focuses on increasing motivation and confidence, assistance with overcoming barriers, identifying social support, and creating specific action plans. Widespread use of short-message services (SMS), commonly known as text messaging, provides one potential tool for researchers to help individuals change difficult behaviors.

Although promising, one of the limitations with current SMS interventions is that most have relied on providing reminder texts at …

Densified Collagen-Fibril Biomaterials For Bone Tissue Engineering, Lauren E. Watkins

Open Access Theses

Millions of craniofacial bone defects occur annually as a result of trauma, congenital defects, disease, or tooth extraction. When present in the oral cavity, these defects are associated with adverse impacts on speech, mastication, and aesthetics. Thus, there is a clinical need for interventional strategies to restore and preserve alveolar bone mass to improve the success of future treatment options intended to reestablish functionality and aesthetics. Guided bone regeneration using bone grafts and a membrane represent the current standard of care for repairing alveolar bone defects, but face a number of limitations related to resorption time and structural integrity. Improvements …

Neural Activity Reveals Effects Of Aging On Inhibitory Processes During Word Retrieval, Ranjini Mohan

Open Access Dissertations

Word retrieval difficulties are one of the most frustrating problems in older adults. Poorer access to phonological (speech sound) representation of the target word has been postulated as the underlying deficit, supported by findings of improvement in word retrieval after phonological priming. But the great variability in naming performance among older adults may reflect cognitive scaffolding or compensatory neurophysiological processes related to maintenance or decline of naming abilities. In order better understand aging effects in the underlying neurophysiological changes associated with phonological retrieval, the present study examined electrophysiological correlates of phonological priming and word retrieval in adults across the lifespan. …