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The Design And Physical Properties Of An Optimized Chitosan Hydrogel For Potential Use In Endodontics, Hunter Housley Apr 2019

The Design And Physical Properties Of An Optimized Chitosan Hydrogel For Potential Use In Endodontics, Hunter Housley

Master's Theses (2009 -)

Introduction: The aim of this study was to synthesize an optimized chitosan/carbon dot hydrogel and measure its effects on the proliferation of dental pulp stem cells. Methods: 1.5% w/v chitosan hydrogel samples were fabricated with 300 mg/ml aqueous β-glycerol phosphate (β-GP) to create an optimized chitosan with a gelation onset temperature below 37°C. Spermidine-functionalized carbon dots (CDs) were added to test groups in concentrations of 25 µg/ml and 50 µg/ml. Complex shear viscosity of the prepared hydrogel samples was evaluated with a Kinexus pro+ shear rheometer (Malvern Panalytical). Samples were evaluated for cellular proliferation with dental pulp stem cells at …


Culture Of Dental Pulp Stem Cells On Nanoporous Alumina Substrates Modified By Carbon Nanotubes, Ameneh Alizadeh, Amir Razmjou, Mehrorang Ghaedi, Ramin Jannesar, Fahimeh Tabatabaei, Vahid Pezeshkpour, Lobat Tayebi Jan 2019

Culture Of Dental Pulp Stem Cells On Nanoporous Alumina Substrates Modified By Carbon Nanotubes, Ameneh Alizadeh, Amir Razmjou, Mehrorang Ghaedi, Ramin Jannesar, Fahimeh Tabatabaei, Vahid Pezeshkpour, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Purpose: Alumina substrates are one of the commonly used scaffolds applied in cell culture, but in order to prevent formation of biofilm on the alumina substrate, these substrates are modified with carbon nanotube. Methods: The alumina substrate was made by a two-step anodization method and was then modified with carbon nanotubes by simple chemical reaction. The substrates were characterized with FTIR, SEM, EDX, 3D laser scanning digital microscope, contact angle (CA) and surface free energy (SFE). To determine how this modification influences the reduction of biofilm, biofilm of two various bacteria, Escherichia coli (E.coli) and Staphylococcus aureus ( …