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Full-Text Articles in Medicine and Health Sciences
If You’Ve Seen One Worm, Have You Seen Them All? Spatial, Community, And Genetic Variability Of Tubificid Communities In Montana, Nilanjan Lodh, Donna M. Rizzo, Billie L. Kerans, Stephanie Mcginnis, Nikolaos Fytilis, Lori Stevens
If You’Ve Seen One Worm, Have You Seen Them All? Spatial, Community, And Genetic Variability Of Tubificid Communities In Montana, Nilanjan Lodh, Donna M. Rizzo, Billie L. Kerans, Stephanie Mcginnis, Nikolaos Fytilis, Lori Stevens
Clinical Lab Sciences Faculty Research and Publications
Genetic studies are recognized increasingly as important for understanding naturally occurring disease dynamics and are used to predict host genetic diversity and coevolutionary processes and to identify species composition in ecological communities. Tubifex tubifex, the definitive host of the whirling disease parasite Myxobolus cerebralis, comprises 6 known lineages that vary widely in parasite susceptibility. We used 16S ribosomal DNA (16S rDNA) to identify relationships among genetic variability of 3 oligochaete genera (T. tubifex, Rhyacodrilus spp., and Ilyodrilus spp.; Oligochaeta:Tubificidae), oligochaete assemblage composition, and the presence of whirling disease in 9 locations across 4 watersheds in Montana, …
Detection Of Parasite-Specific Dna In Urine Sediment Obtained By Filtration Differentiates Between Single And Mixed Infections Of Schistosoma Mansoni And S. Haematobium From Endemic Areas In Ghana, Nilanjan Lodh, Jean M. Naples, Kwabena M. Bosompem, Joseph Quartey, Clive J. Shiff
Detection Of Parasite-Specific Dna In Urine Sediment Obtained By Filtration Differentiates Between Single And Mixed Infections Of Schistosoma Mansoni And S. Haematobium From Endemic Areas In Ghana, Nilanjan Lodh, Jean M. Naples, Kwabena M. Bosompem, Joseph Quartey, Clive J. Shiff
Clinical Lab Sciences Faculty Research and Publications
Differential diagnosis of Schistosoma mansoni and S. haematobium, which often occur sympatrically in Africa, requires both urine and stool and the procedures are low in sensitivity. The standard diagnostic tests, such as Kato-Katz (KK) for S. mansoni eggs and presence of haematuria for S. haematobium both lack sensitivity, produce false-negative results and show reduced accuracy with decreasing intensity of infection. The need for a single diagnostic test with high sensitivity and specificity for both parasites is important as many African countries are implementing Mass Drug Administration (MDA) following recommendations of the World Health Organization (WHO). Eighty-six samples of urine …
Diagnosis Of Schistosoma Mansoni Without The Stool: Comparison Of Three Diagnostic Tests To Detect Schiostosoma Mansoni Infection From Filtered Urine In Zambia, Nilanjan Lodh, James C. L. Mwansa, Mabel M. Mutengo, Clive J. Shiff
Diagnosis Of Schistosoma Mansoni Without The Stool: Comparison Of Three Diagnostic Tests To Detect Schiostosoma Mansoni Infection From Filtered Urine In Zambia, Nilanjan Lodh, James C. L. Mwansa, Mabel M. Mutengo, Clive J. Shiff
Clinical Lab Sciences Faculty Research and Publications
Diagnosis for intestinal Schistosoma mansoni lacks sensitivity and is arduous to conduct. The standard diagnostic tests, Kato-Katz (KK) and circulating cathodic antigen (CCA) both lack sensitivity and with KK, require obtaining, transporting, and examining fresh stool. We compared diagnostic efficacy of KK, CCA, and polymerase chain reaction (PCR) to detect S. mansoni infection (species-specific DNA) from 89 filtered urine samples collected in Zambia. The PCR was the strongest indicator of positive cases with sensitivity and specificity of 100% in comparison to CCA (67% and 60%) and KK (50% and 100%). High positive and negative predictive values (100%) were also indicative …
The Parasite That Causes Whirling Disease, Myxobolus Cerebralis, Is Genetically Variable Within And Across Spatial Scales, Nilanjan Lodh, Billie L. Kerans, Lori Stevens
The Parasite That Causes Whirling Disease, Myxobolus Cerebralis, Is Genetically Variable Within And Across Spatial Scales, Nilanjan Lodh, Billie L. Kerans, Lori Stevens
Clinical Lab Sciences Faculty Research and Publications
Understanding the genetic structure of parasite populations on the natural landscape can reveal important aspects of disease ecology and epidemiology and can indicate parasite dispersal across the landscape. Myxobolus cerebralis (Myxozoa: Myxosporea), the causative agent of whirling disease in the definitive host Tubifex tubifex, is native to Eurasia and has spread to more than 25 states in the USA. The small amounts of data available to date suggest that M. cerebralis has little genetic variability. We examined the genetic variability of parasites infecting the definitive host T. tubifex in the Madison River, MT, and also from …