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Efficacy Of Chitosan As Final Irrigating Solution On Tgf-Β1 Release From Root Canal Dentin And Its Effect On Dental Pulp Stem Cells Response, Asmaa Abd El-Hady, Wael Kamel, Mona Farid, Dina Sabry
Efficacy Of Chitosan As Final Irrigating Solution On Tgf-Β1 Release From Root Canal Dentin And Its Effect On Dental Pulp Stem Cells Response, Asmaa Abd El-Hady, Wael Kamel, Mona Farid, Dina Sabry
Al-Azhar Journal of Dentistry
Purpose: To study the effect of Chitosan final irrigating solution compared with EDTA using different irrigation techniques, on TGF-β1 release from root canal dentin, using enzyme-linked immunosorbent assay (ELISA), and to evaluate the effect of released TGF-β1 on Dental Pulp Stem Cells. Materials and Methods: Human DPSCs were isolated from impacted third molars. Forty eight human root segments were prepared to a standardized truncated cone-shaped canal with an open apex of 1 mm diameter, the volume of prepared segments were determined using CBCT. Samples were randomized into two experimental groups (n=24) regarding the final irrigating solution; Group I: 1.5% NaOCl …