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Estimation Of Percentage Of Morbidity And Mortality Due To Avian Influenza (H9) At Commercial Poultry Layer Farms Of Lahore, Afeefa Shafiq, Aisha Arshad, Zafar-Ul-Ahsan Qurashi, Zain Khalid, Noor Ul Huda, Muhammad Noman, Muhammad Ilyas, Hamad Bin Rashid, Asim Khalid Mehmood, Saima Hasan, Quratul Ain, Sobia Amir, Sheraz Shahid, Shakera Sadiq, Mamoona Chaudhry
Estimation Of Percentage Of Morbidity And Mortality Due To Avian Influenza (H9) At Commercial Poultry Layer Farms Of Lahore, Afeefa Shafiq, Aisha Arshad, Zafar-Ul-Ahsan Qurashi, Zain Khalid, Noor Ul Huda, Muhammad Noman, Muhammad Ilyas, Hamad Bin Rashid, Asim Khalid Mehmood, Saima Hasan, Quratul Ain, Sobia Amir, Sheraz Shahid, Shakera Sadiq, Mamoona Chaudhry
The Thai Journal of Veterinary Medicine
The current study was aimed at the estimation of the percentage of morbidity, mortality and potential risk factors associated with the avian influenza virus (AIV) H9 at commercial layer farms of District Lahore, Pakistan. Samples collected included blood samples and tracheal/oropharyngeal swabs from sick birds and tissue samples (trachea & lungs) of dead birds. Sera were tested by Hemagglutination Inhibition Test (HI) using the positive control antigen of H9. Tracheal/oropharyngeal swabs and tissue samples were processed by real-time RT-PCR (rRT-PCR). Out of 50 layer farms, 16 farms were positive for antibody titer against the AIV H9. Out of a total …
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan, Maria Komal, Kiran Afshan, Sabika Firasat, Màrius V. Fuentes
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan, Maria Komal, Kiran Afshan, Sabika Firasat, Màrius V. Fuentes
The Thai Journal of Veterinary Medicine
This study determined the molecular characterization of Paramphistomum epiclitum using the second internal transcribed spacer (ITS-2) grouping and secondary structure analysis from buffaloes in Khyber Pakhtunkhwa province, Pakistan. Paramphistomes were collected from the rumen of 25 infected buffaloes and their DNA was separated. DNA was intensified, followed by sequencing. Phylogenetic examinations were carried out using the Maximum Composite Likelihood approach. The results revealed extensive intraspecific and interspecific varieties among different paramphistomid species. The sequences in the present study (GenBank accession number: MW481321 and MW481322) were compared to 46 reference sequences of Paramphistomum and other trematode data from GenBank. The results …