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Non-Invasive Prenatal Determination Of Fetal Rhd Genotyping From Maternal Plasma: A Preliminary Study In Pakistan, Nuruddin Badruddin Mohammed, Fatima Kakal, Mehreen Somani, Wajiha Zafar
Non-Invasive Prenatal Determination Of Fetal Rhd Genotyping From Maternal Plasma: A Preliminary Study In Pakistan, Nuruddin Badruddin Mohammed, Fatima Kakal, Mehreen Somani, Wajiha Zafar
Department of Obstetrics & Gynaecology
Objectives: To determine the accuracy of the non-invasive pre-natal real-time polymerase chain reaction based fetal RhD genotyping from maternal plasma.
Study Design: Cross-sectional study.
Place And Duration Of Study: Juma Health Sciences Research Laboratory, The Aga Khan University Hospital, Karachi, from July to December 2008.
Methodology: Cell-free plasma DNA from 21 D-negative women with D-positive spouse between 20-39 weeks of gestation was tested for the presence of exon 5 region of RhD gene using real-time polymerase chain reaction. b-globin was employed as the house-keeping gene. Sensitivity and specificity of the real-time PCR-based non-invasive fetal RhD genotyping was obtained by calculating …