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Alcohol-Induced Suppression Of Gluconeogenesis Is Greater In Ethanol Fed Female Rat Hepatocytes Than Males, Ken D. Sumida, Alma A. Cogger, Aleksey V. Matveyenko

Physical Therapy Faculty Articles and Research

The impact of alcohol-induced suppression on hepatic gluconeogenesis (HGN) after chronic ethanol consumption between males and females is unknown. To determine the effects of chronic alcohol consumption (8 weeks) on HGN, the isolated hepatocyte technique was used on 24 h fasted male and female Wistar rats. Livers were initially perfused with collagenase and the hepatocytes were isolated. Aliquots of the cell suspension were placed in Krebs-Henseleit buffer and incubated for 30 min with lactate, [U-C-14]lactate, and nine different concentrations of ethanol (EtOH). Dose-effect curves were generated for the determination of maximal and half-maximal alcohol-induced inhibition on HGN. There was no …