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Full-Text Articles in Medicine and Health Sciences
Recovery Of Urothelial Mediator Release But Prolonged Elevations In Interleukin-8 And Nitric Oxide Secretion Following Mitomycin C Treatment, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Recovery Of Urothelial Mediator Release But Prolonged Elevations In Interleukin-8 And Nitric Oxide Secretion Following Mitomycin C Treatment, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Catherine M. McDermott
Intravesical mitomycin C (MMC) is commonly used to treat bladder cancer but is associated with local adverse effects. Here, we investigate the effects of MMC on release of urothelial mediators and production of inflammatory cytokines. Recovery and the effects of repeat treatment were also investigated. Urothelial cells were treated with MMC for 2 h at 37 °C. Immediately, 24 h and 7 days following MMC treatment, effects were assessed in terms of changes in ATP, acetylcholine and PGE2 release, and the presence of inflammatory cytokines and nitric oxide in incubation medium. Endpoints were also assessed 7 days after repeat MMC …
Recovery Of Urothelial Mediator Release But Prolonged Elevations In Interleukin-8 And Nitric Oxide Secretion Following Mitomycin C Treatment, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Recovery Of Urothelial Mediator Release But Prolonged Elevations In Interleukin-8 And Nitric Oxide Secretion Following Mitomycin C Treatment, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Russ Chess-Williams
Intravesical mitomycin C (MMC) is commonly used to treat bladder cancer but is associated with local adverse effects. Here, we investigate the effects of MMC on release of urothelial mediators and production of inflammatory cytokines. Recovery and the effects of repeat treatment were also investigated. Urothelial cells were treated with MMC for 2 h at 37 °C. Immediately, 24 h and 7 days following MMC treatment, effects were assessed in terms of changes in ATP, acetylcholine and PGE2 release, and the presence of inflammatory cytokines and nitric oxide in incubation medium. Endpoints were also assessed 7 days after repeat MMC …
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Catherine M. McDermott
Intravesical treatment with cytotoxic drugs such as doxorubicin is associated with local adverse effects in bladder cancer patients. Here we investigate the effects of doxorubicin on urothelial release of ATP, acetylcholine and prostaglandin E2, and production of inflammatory cytokines. Urothelial cells were treated with doxorubicin for 1 h at 37 °C. Immediately or 24 h following treatment the level of ATP, acetylcholine and prostaglandin E2 released under basal and stimulated conditions was measured and compared to release from vehicle treated control cultures. The presence of inflammatory cytokines, in culture medium was also assessed 24 h after doxorubicin pre-treatment. Immediately following …
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Induction Of Inflammatory Cytokines And Alteration Of Urothelial Atp, Acetylcholine And Prostaglandin E2 Release By Doxorubicin, Sung-Hung Kang, Russ Chess-Williams, Shailendra Anoopkumar-Dukie, Catherine Mcdermott
Russ Chess-Williams
Intravesical treatment with cytotoxic drugs such as doxorubicin is associated with local adverse effects in bladder cancer patients. Here we investigate the effects of doxorubicin on urothelial release of ATP, acetylcholine and prostaglandin E2, and production of inflammatory cytokines. Urothelial cells were treated with doxorubicin for 1 h at 37 °C. Immediately or 24 h following treatment the level of ATP, acetylcholine and prostaglandin E2 released under basal and stimulated conditions was measured and compared to release from vehicle treated control cultures. The presence of inflammatory cytokines, in culture medium was also assessed 24 h after doxorubicin pre-treatment. Immediately following …