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Articles 1 - 4 of 4
Full-Text Articles in Medicine and Health Sciences
Comparison Of A Quantitative Microtiter Method, A Quantitative Automated Method, And The Plate-Count Method For Determining Microbial Complement Resistance, Margie D. Lee, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan, Emmett B. Shotts Jr.
Comparison Of A Quantitative Microtiter Method, A Quantitative Automated Method, And The Plate-Count Method For Determining Microbial Complement Resistance, Margie D. Lee, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan, Emmett B. Shotts Jr.
Lisa K. Nolan
A quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of data generated by the automated system and plate-count techniques resulted in a classification of the microorganisms into three groups: those sensitive to the action of complement; those of intermediate sensitivity to the action of complement; and those resistant to the action of complement. Although the …
Comparison Of Chicken Plasma And Guinea Pig Serum In A Quantitative Microtiter Method Of Determining Microbial Complement Resistance, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan
Comparison Of Chicken Plasma And Guinea Pig Serum In A Quantitative Microtiter Method Of Determining Microbial Complement Resistance, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan
Lisa K. Nolan
Y. A quantitative microtiter method using chicken plasma is described for determining the degree of complement resistance or sensitivity of avian Escherichia coli isolates. Results obtained with the microtiter method using chicken plasma were compared with results obtained using commercially available standardized guinea pig serum as the source of complement. The test organisms consisted of five isolates of E. coli isolated from chickens. Three isolates were from flocks with colisepticemia; one was from a flock with omphalitis; and one isolate was a non-pathogenic control. Data were accumulated from the five avian E. coli isolates incubated at 35 C with either …
Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz
Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz
School of Veterinary and Biomedical Sciences: Faculty Publications
An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein …
Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson
Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson
School of Veterinary and Biomedical Sciences: Faculty Publications
Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.