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Full-Text Articles in Medicine and Health Sciences
Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong
Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong
Master's Theses
Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …
Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge
Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge
Storrs Agricultural Experiment Station
No abstract provided.