Medicine and Health Sciences Commons^™

Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The high incidence of multidrug-resistant Salmonella Typhimurium and Salmonella Kentucky isolates is a concern for human and animal health. This study aimed to investigate the antibiotic resistance determinants of a total of 150 S. Typhimurium and S. Kentucky isolates obtained from cows, calves, lambs, and poultry. Salmonella isolates were tested against 13 different antimicrobials using the Kirby-Bauer disc diffusion method, and genotypic antimicrobial resistance determinants were investigated by polymerase chain reaction. Significant differences were detected among serovars for gentamicin, streptomycin, ampicillin, sulfonamide, nalidixic acid, ciprofloxacin, and tetracycline resistance, with the rates of resistance to these antibiotics being determined to be …

H5n1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b In Wild And Domestic Birds: Introductions Into The United States And Reassortments, December 2021–April 2022, Sungsu Youk, Mia Kim Torchetti, Kristina Lantz, Julianna B. Lenoch, Mary Lea Killian, Christina Leyson, Sarah N. Bevins, Krista Dilione, Hon S. Ip, David E. Stallknecht, Rebecca L. Poulson, David L. Suarez, David E. Swayne, Mary J. Pantin-Jackwood

USDA Wildlife Services: Staff Publications

Highly pathogenic avian influenza viruses (HPAIVs) of the A/goose/Guangdong/1/1996 lineage H5 clade 2.3.4.4b continue to have a devastating effect on domestic and wild birds. Full genome sequence analyses using 1369 H5N1 HPAIVs detected in the United States (U.S.) in wild birds, commercial poultry, and backyard flocks from December 2021 to April 2022, showed three phylogenetically distinct H5N1 virus introductions in the U.S. by wild birds. Unreassorted Eurasian genotypes A1 and A2 entered the Northeast Atlantic states, whereas a genetically distinct A3 genotype was detected in Alaska. The A1 genotype spread westward via wild bird migration and reassorted with North American …

H7n9 Influenza A Virus Transmission In A Multispecies Barnyard Model, Angela M. Bosco-Lauth, Anna Rodriguez, Rachel M. Maison, Stephanie M. Porter, J. Jeffrey Root

USDA Wildlife Services: Staff Publications

Influenza A viruses are a diverse group of pathogens that have been responsible for millions of human and avian deaths throughout history. Here, we illustrate the transmission potential of H7N9 influenza A virus between Coturnix quail (Coturnix sp.), domestic ducks (Anas platyrhynchos domesticus), chickens (Gallus gallus domesticus), and house sparrows (Passer domesticus) co-housed in an artificial barnyard setting. In each of four replicates, individuals from a single species were infected with the virus. Quail shed virus orally and were a source of infection for both chickens and ducks. Infected chickens transmitted the virus to quail but not to ducks or …

Antibody Dependent Enhancement Of Infectious Bronchitis Virus In Poultry, Zachary Hamilton, Barry Simpson Dr., Donald L. Reynolds, Dvm, Dr.

UCARE Research Products

Avian infectious bronchitis (IB) is a coronavirus infection of chickens that causes respiratory disease and reproductive problems in chickens. Currently, there are vaccines that are effective against IB. However, new variants and strains of avian infectious bronchitis virus (IBV) routinely emerge. A vaccine that is not the same strain as the virus is not completely effective in protecting against other variants because the vaccine will not allow the host antibodies to completely neutralize the strain. This is a problem because it makes IB difficult to control and diagnose.

Antibody-dependent enhancement (ADE) is a phenomenon whereby non-neutralizing antibodies, or low levels …

Strength In Numbers: Avian Influenza A Virus Transmission To Poultry From A Flocking Passerine, J. Jeffrey Root, Jeremy W. Ellis, Susan A. Shriner

USDA Wildlife Services: Staff Publications

The effects of flock size of European starlings (Sturnus vulgaris) was experimentally manipulated to assess the potential of influenza A virus (IAV; H4N6) transmission from a flocking passerine to bobwhite quail (Colinus virginianus) through shared food and water resources to mimic starling intrusions into free-range and backyard poultry operations. Of the three starling flock sizes tested (n = 30, n = 20 and n = 10), all successfully transmitted the virus to all or most of the quail in each animal room (6/6, 6/6 and 5/6) by the end of the experimental period, as determined by seroconversion and/or viral RNAshedding. …

Typing Of Rarely Isolated Salmonella Serotypes With Mlst, Seyyi̇de Sariçam İnce, İnci̇ Başak Müştak, Hami̇t Kaan Müştak

Turkish Journal of Veterinary & Animal Sciences

Salmonellosis caused by nontyphoidal Salmonella is the second most common zoonotic infection worldwide. The major foodborne route of this infection is the consumption of contaminated poultry meat and egg. Therefore, monitoring and control of Salmonella agents in poultry populations is critical to reduce the risks for public health. In the poultry industry, the most common serotypes in Salmonella infections are Salmonella enterica subsp. enterica serotype Enteritidis and Typhimurium. In recent years, other frequently isolated Salmonella serotypes have also increased in Turkey because of the Salmonella control programs regulated by many countries. Hence, other Salmonella serotypes (except for the most frequently …

H7n1 Low Pathogenicity Avian Influenza Viruses In Poultry In The United States During 2018, Dong-Hun Lee, Mary Lea Killian, Thomas J. Deliberto, Xiu-Feng Wan, Li Lei, David E. Swayne, Mia Kim Torchetti

USDA Wildlife Services: Staff Publications

Here, we report three detections of H7N1 low pathogenicity avian influenza viruses (LPAIV) from poultry in Missouri (n¼2) and Texas (n¼1) during February and March 2018. Complete genome sequencing and comparative phylogenetic analysis suggest that the H7 LPAIV precursor viruses were circulating in wild birds in North America during the fall and winter of 2017 and spilled over into domestic poultry in Texas and Missouri independently during the spring of 2018.

RESUMEN. Nota de investigacio´n—Virus de la influenza aviar de baja patogenicidad H7N1 en avicultura, Estados Unidos, 2018. En este art´ıculo se reportan tres detecciones del virus de influenza aviar …

Phylogrouping And Antimicrobial Resistance Analysis Of Extraintestinal Pathogenic Escherichia Coli Isolated From Poultry Species, Sujata Bhave, Rahul Kolhe, Ranjan Mahadevaswamy, Chandrakant Bhong, Samir Jadhav, Saba Nalband, Dattatray Gandhale, Dushyant Muglikar

Turkish Journal of Veterinary & Animal Sciences

This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenic Escherichia coli (ExPEC) in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened by PCR for detection of ExPEC strains. Nineteen (13.01%) isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealed as follows: broiler (25%), layer (15.87%), and raw chicken (3.12%). Turkey, duck, and water samples were negative for ExPEC strains. ExPEC strains belonged to phylogenetic groups B2 (52.63%), A (36.84%), and D (10.53%). Twenty-three (15.75%) isolates were ESBL-positive, including four ExPEC strains. ESBL-positive …

A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi

Turkish Journal of Veterinary & Animal Sciences

Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant, Harun Hizlisoy, Serhat Al, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan, Kadi̇r Semi̇h Gümüşsoy

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to investigate the presence of Escherichia coli O157 and to determine the antibiotic susceptibility of isolates in different poultry-originated materials. In total 300 samples of chicken carcass swabs (n = 50), chicken feces (n = 50), gizzard (n = 50), liver (n = 50), water in the final scald tank (n = 50), and equipment such as tables, conveyers, and knives (n = 50) were used as materials. Overall, 45 E. coli isolates were obtained from the 300 poultry samples. However, 13 (4.3%) of the samples were identified as E. coli O157 including 3 …

Prevalence Of Aada1, Aada2, Aadb, Stra And Strb Genes And Their Associations With Multidrug Resistance Phenotype In Salmonella Typhimurium Isolated From Poultry Carcasses, Abbas Doosti, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Mokhtari-Farsani

The Thai Journal of Veterinary Medicine

This study aimed to assess the prevalence of aminoglycoside resistance genes in S. Typhimurium isolated from poultry carcasses in Iran, and to reveal the most prevalent patterns of antimicrobial resistance. A total number of 300 samples of poultry carcasses were analyzed. Salmonella was isolated from 245 samples (81.66%). Multiplex PCR showed that 56.3% of the samples belonged to serovar S. Typhimurium and the remainder (43.6%) contained the rest of serovars. The highest rate of drug resistance was observed for tetracycline (97.0%), nalidixic acid (87.0%) and amoxicillinclavulanic acid (67.4%). These serovars, however, were sensitive to cefotaxime (84.8%), sulfamethoxazole trimethoprim (77.6%) and …

Surveillance, Epidemiological, And Virological Detection Of Highly Pathogenic H5n1 Avian Influenza Viruses In Duck And Poultry From Bangladesh, Wahedul Karim Ansari, Md Safiullah Parvej, Mohamed E. El Zowalaty, Sally Jackson, Stephen A. Bustin, Adel K. Ibrahim, Md Tanvir Rahman, Han Zhang, Mohammad Ferdousur Rahman Khan, Md Mostakin Ahamd, Md. Fasiur Rahman, Marzia Rahman, Khm Nazmul H. Nazir, Sultan Ahmed, Md Liakot Hossenn, Md Abdul Kafi, Mat Yamage, Nitish C. Debnath, Graba Ahmed, Hossam Ashour, Md Masoud, Ayman Noreddin, Md B. Rahman

Pharmacy Faculty Articles and Research

Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2191 oropharyngeal swabs as well as 1725 sera samples were collected from live birds including duck and chicken in different locations in Bangladesh between the years of 2013 and 2014. Samples were tested using virus isolation, serological tests and molecular methods of RT-PCR. …

Appraisal Of The Impact Of Aluminosilicate Use On The Health And Performance Of Poultry, Ali Gilani, Hassan Kermanshahi, Abolghasem Golian, Saeed Seifi

Turkish Journal of Veterinary & Animal Sciences

Aluminosilicates are feed additives used for multiple purposes in the poultry industry. Bentonites, zeolites, and kaolins are types of aluminosilicate compounds that appear in the marketplace due to their capabilities as mycotoxin adsorbents and other qualifications. While there are some differences among their efficacies, anecdotal evidence confirms that these additives ameliorate aflatoxicosis and manure ammonia emission, and improve the durability of pelleted diets. Furthermore, these supplements are safe at 0.2%-1% inclusion levels in the commercial diet of broilers and layers and did not have any adverse effects on productive indices, and even promoted birds' performance. Nevertheless, their upper levels, especially …

Evaluación De La Actividad De Enfermedades Virales En Explotaciones De Ponedoras Comerciales De Fómeque, Cundinamarca, Andrés Felipe Santander Torres

Maestría en Ciencias Veterinarias

Mundialmente el sector avícola ha sido de gran importancia tanto en su impacto en seguridad alimentaria como en sus aportes para el desarrollo económico. En los últimos 20 años el sector avícola se ha consolidado en una de las producciones más dinámica dentro de Colombia. Sin embargo este desarrollo se puede afectar por la presentación de enfermedades infecciosas, es por esto que se pretende evaluar la actividad de enfermedades virales en explotaciones de ponedoras comerciales de Fómeque Cundinamarca. Se realizó la toma de muestras a la mitad y al final del periodo de cría y levante de las ponedoras, con …

Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed …

Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad

Turkish Journal of Veterinary & Animal Sciences

In western Algeria, 251 Escherichia coli isolates were recovered from broilers with clinical signs and lesions of colibacillosis. Serotyping showed that 82% of the isolates belong to one of the following serotypes: O78, O2, and O1. Antibiograms revealed a high level of resistance to oxytetracycline (82%), amoxicilline and ampicilline (47%), trimethoprim-sulfmethoxazole (42%), oxolinic acid and flumequine (31%). However, only a low percentage of strains were resistant to enrofloxacine (6%) and colistine (3%). Although multiresistance was common, low association was found between the serotype and antibioresistance.

Detection Of Antibodies Produced Against Ornithobacterium Rhinotracheale And Bordetella Avium By Enzyme-Linked Immunosorbent Assay In Hens And Turkeys In Aydın Province, Turkey, Süheyla Türkyilmaz, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

Respiratory diseases are very common in poultry. Bordetella avium and Ornithobacterium rhinotracheale are among the major microorganisms responsible for these diseases. The study was conducted to investigate the seroprevalence of O. rhinotracheale and B. avium in Aydın. Two hundred seventy-six chicken and 360 turkey serum samples were collected from poultry with respiratory disease suspicion in poultry enterprises around the city and samples were then examined for the presence of B. avium and O. rhinotracheale by commercial ELISA kits. The results revealed that 66.3% of chicken and 11.1% of turkey serum samples were positive for O. rhinotracheale and 29.1% of turkey …

Isolation And Serotyping Of Ornithobacterium Rhinotracheale From Poultry, Süheyla Türkyilmaz

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were the isolation of Ornithobacterium rhinotracheale from poultry and serotyping of the isolates and field sera by agar gel precipitation (AGP) test. For the isolation 257 chickens and 214 turkeys were used and for the AGP test 333 chickens and 250 turkeys were used. O. rhinotracheale was isolated from the sinus discharge of 3 broiler breeders at 37, 42 and 46 weeks of age. It was determined that 3 isolates were O. rhinotracheale serotype I having cross reactions with serotype L. Of 333 sera examined 86 were found seropositive in chickens by AGP test. It …

Physiological Reactions Of Poultry To Heat Stress And Methods To Reduce Its Effects On Poultry Production, Narongsak Chaiyabutr

The Thai Journal of Veterinary Medicine

Thermal environment is regarded as the prime factor governing the production because, both the feed consumption and heat production are related closely to the thermal environment. Therefore, heat stress is caused by a combination of environmental and animal factors that result in reduced performance as the animal attempts to cool itself. In this review, the physiological reactions of bodily functions during heat exposure in poultry are described. Thermal panting, the marked response of poultry to heat stress, is compensatory behavior to permit a high rate of evaporative cooling from the respiratory tract. Cardiorespiratory responses to thermal stress show different patterns …

Rapid Identification Of Avian Salmonella Isolates By Air Thermal Cycler Amplification And Capillary Gel Electrophoresis, K. Tayfun Çarli, Vi̇ldan Caner, Ayşegül Eyi̇gör

Turkish Journal of Veterinary & Animal Sciences

The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within …

The Effects Of Some Antibacterial Drugs On The Immune System Of Poultry, Yusuf Şanli, Ali̇ Bi̇lgi̇li̇, Ayhan Fi̇lazi̇, Ender Yarsan, Reşat Aşti, Nevi̇n Kurtdede, Hakan Yardimci

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of some different antibacterial drugs used in the poultry industry for their effects on the immune system of poultry. This experiment was carried on a hundred and fifty Ross PM-3 type, daily broiler chicks. Oxytetracycline and amoxicillin used in treatment of drinking water, and zinc bacitracin and salinomycin capable of being continuously added to feed in anabolic and preservative concentrations were chosen as antibacterial drug samples. In the first 21 days of their lives the broilers were fed with a chick starter feed, that did not contain any antibacterial or anticoccidial agents, …

Endogenous Amino Acid Losses In Pigs And Poultry: A Review(การสูญเสียกรดอะมิโนภายในระบบทางเดินอาหารของไก่และสุกร : บทความ), Kris Angkanaporn, Wayne L. Bryden, Velmurugu Ravindran

The Thai Journal of Veterinary Medicine

Considerable quantities of non-dietary protein are secreted into the digesta of pigs and poultry. This protein is defined as the protein found in the chyme or faeces when a nitrogen-free diet is fed to animals. Endogenous proteins comprise protein, peptides, amino acids and nitrogen-containing substances from saliva, bile, pancreatic, gastric and intestinal secretions; plasma and sloughed epithelial cells. These endogenous proteins are digested and absorbed in a manner similar to those of dietary origin. Conventional approaches to the estimation of obligatory loss have included the measurement of amino acid excretion in fasted animals, in animals fed on protein-free diets, or …

Pollorum Tested Poultry Flocks, 1966-67, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

PULLORUM disease has been virtually eliminated from breeding and hatchery poultry flocks in Western Australia by the annual blood-testing of all birds and the destruction of reactors.

Pullorum Tested Poultry Flocks, 1965, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

PULLORUM disease has been virtually eliminated from breeding and hatchery poultry flocks in Western Australia by the annual blood-testing of all birds and the destruction of reactors.

Control Of Lice On Poultry, B R. Vale

Journal of the Department of Agriculture, Western Australia, Series 4

DURING February and March of 1965 some particularly heavy body lice infestations were found on fowls in both deep litter and cage units in Western Australia.

In some instances comparatively new laying cage units were involved.

The most seriously affected flocks showed marked unthriftiness and depressed egg production.

Liver Cirrhosis : A New Poultry Disease, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

A new poultry disease which is apparently peculiar to Western Australia is causing losses in some commercial poultry flocks in this State.

The cause of the disease has not been isolated and no reliable treatment can yet be recommended.

Pullorum Tested Poultry Flocks : 1964, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

PULLORUM disease has been virtually eliminated from breeding and hatchery poultry flocks in Western Australia by the annual blood-testing of all birds and the destruction of reactors.

Precautions For Day Old Fowl Pox Vaccination, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

DURING 1962 day old vaccination was widely adopted as a preventative measure against fowl pox.

From June, 1961, until mid-June, 1962, this procedure proved to be highly successful, but from this date onwards a proportion of treated flocks became affected by breakdowns accompanied by mortality and retarded growth.

In some cases the losses were disturbingly high.

Water For Agriculture. 5. Salt Poisoning In Livestock, J Craig

Journal of the Department of Agriculture, Western Australia, Series 4

LIVESTOCK need a plentiful supply of good, fresh drinking water for normal health and high production.

Over large areas of Western Australia the water from bores, springs and wells contains varying but appreciable amounts of salt and special care may be needed to ensure that the stock water supply is satisfactory.