Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Publication Year
- Publication
- Publication Type
Articles 1 - 24 of 24
Full-Text Articles in Medicine and Health Sciences
A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir
A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir
Turkish Journal of Veterinary & Animal Sciences
In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …
A Study On Oocyte Development Of The Atlantic Bluefin Tuna(Thunnus Thynnus, Linnaeus 1758) Under Farming Conditions, Şükrü Yildirim, Sema Mi̇di̇lli̇, Mehmet Güler, Ridvan Cenk Yurttaş, Deni̇z Çoban
A Study On Oocyte Development Of The Atlantic Bluefin Tuna(Thunnus Thynnus, Linnaeus 1758) Under Farming Conditions, Şükrü Yildirim, Sema Mi̇di̇lli̇, Mehmet Güler, Ridvan Cenk Yurttaş, Deni̇z Çoban
Turkish Journal of Veterinary & Animal Sciences
Body length and weight measurements and histological samples were collected from slaughtered Atlantic bluefin tuna (Thunnus thynnus) that were fattening under farming conditions for the determination of oocyte development in February 2015 (n = 10), May 2015 (n = 1), June 2015 (n = 1), July 2015 (n = 9), and July 2016 (n = 3) during harvest and soon after newly dead. The developmental phases of oocytes were evaluated based on their diameters, fork body length and weight of fish, surface water temperature, and species' reproduction season. According to the histological results, the oocytes were at the beginning of …
Evaluation Of Oocyte Developmental Competence And Potential Strategies To Improve Oocyte Quality, Min Yang
Evaluation Of Oocyte Developmental Competence And Potential Strategies To Improve Oocyte Quality, Min Yang
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Assisted reproductive technologies (ARTs) have now been extensively used to promote reproductive efficiency as a fertility treatment not only in human medicine but also animal reproduction. ARTs serve as an important tool to advance the fundamental knowledge of reproductive processes. The quality of female’s eggs defines its ability to undergo maturation, fertilization, and development. This quality is determined by various factors and is crucial for the success of ARTs. Any alternations happening during the egg growth and maturation process can result in the decreased quality, which could have long-lasting effects on development. Improving the developmental efficiency of the egg is …
Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala
Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala
Turkish Journal of Veterinary & Animal Sciences
In vitro fertilization is a common method of fertilization in the case of both humans and animals. Although this method has developed very rapidly, there are still many unanswered questions about the optimal characteristics that oocytes should have for performing this procedure. The object of this study was to describe the correlation between the diameter and size of swine oocytes in relation to pubescence. The research was conducted on ovaries of piglets (n = 65) and adult swine (n = 69). Each oocyte was examined with and without the zona pellucida. The analysis showed that the average size of the …
Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu
Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
In vitro embryo production has proven as potential tool for understanding factors involving developmental biology of embryos. This study aimed at examining the effects of recombinant human follicle stimulating hormone (rhFSH) on meiotic resumption and also on developmental competence of cat oocytes. Experiment 1 studied mRNA expression of luteinizing hormone (LH), follicle stimulating hormone (FSH), epidermal growth factor (EGF) and insulin like growth factor-1 (IGF-1) receptors (R) in cumulus cells. mRNA expression of LH receptor in cumulus cells after oocyte maturation was additionally examined. Experiment 2 examined the effect of different rhFSH concentrations (0.01, 0.05, 0.1 and 1 IU/ml) on …
The Effect Of Oviductal Cells On In Vitro Maturation Of Canine Oocytes In Different Culture Media, Si̇nem Özlem Engi̇nler, Asi̇ye İzem Sandal, Özen Banu Özdaş, Ramazan Arici, Ezgi̇ Ertürk, Eli̇f Merve Çinar, Israa Faris Mohammed, Alper Baran, Çağatay Tek, Mehmet Can Gündüz
The Effect Of Oviductal Cells On In Vitro Maturation Of Canine Oocytes In Different Culture Media, Si̇nem Özlem Engi̇nler, Asi̇ye İzem Sandal, Özen Banu Özdaş, Ramazan Arici, Ezgi̇ Ertürk, Eli̇f Merve Çinar, Israa Faris Mohammed, Alper Baran, Çağatay Tek, Mehmet Can Gündüz
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. The oviducts were flushed with TCM-199 containing 10% FCS and were scraped and squeezed into a tube …
The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao
The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao
Turkish Journal of Veterinary & Animal Sciences
Recent progress in genomic analysis and other biochemical methods has led to the discovery of a large population of microRNAs (miRNAs), which have been demonstrated to play important roles in diseases and a wide range of developmental processes. Characterization of miRNA expression profiles in different stages of ovarian follicular development and early embryogenesis has suggested the potential roles of miRNAs in follicular development, maturation of oocytes, and preimplantation embryonic development. This review focuses on the current studies of miRNAs involved in ovarian and early embryonic development in cattle.
The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson
The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson
Turkish Journal of Veterinary & Animal Sciences
This review focuses on recent research related to the avian ovary and, in particular, cellular mechanisms mediating embryonic growth of the ovary, postembryonic follicular development, and the process of follicular selection. Attention is drawn to a variety of fundamental, yet unresolved, aspects of ovarian function, several of which are uniquely related to avian reproductive strategies. Some notable differences in ovarian function between birds and the most closely related extant group, the crocodilians, are illustrated. Moreover, because the sauropsid (reptilian and avian) and synapsid (mammalian) lineages diverged shortly after the emergence of amniotes some 340 million years ago, consideration is given …
Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes, Lakshman Sahoo, Suresh K. Singla
Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes, Lakshman Sahoo, Suresh K. Singla
Turkish Journal of Veterinary & Animal Sciences
Recovery rate and quality of oocytes were investigated from abattoir collected ovaries with and without corpora lutea (CL). The oocytes were categorized into four grades: grade-A, grade-B, grade-C, and grade-D based on the presence of the cumulus cells complex around the oocytes. The number of oocytes per ovary were 1.7 ±0.1 and 1.5 ±0.1, obtained from ovaries without CL and with CL, respectively. Recovery rate of different grades of oocytes from ovaries with CL and without CL were almost identical.
Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak
Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 °C and 32 °C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 °C, group A2: 32 °C [control]; group B1: 4 °C, group B2: 32 °C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm2. For groups B1 and B2, 1-2 cortex pieces …
In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi
In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi
Turkish Journal of Veterinary & Animal Sciences
Conditions for in vitro maturation (IVM) of primary oocytes without conventionally used fetal calf serum and hormones were optimized in order to reduce the cost of laboratory produced buffalo embryos. Comparisons were made between oocyte recovery methods (aspiration vs. slicing) and IVM in medium 199 (static culture method vs. flux culture method) supplemented with 4-5 × 106 granulosa cells mL-1 that contained either estrus buffalo serum (EBS) or fetal calf serum (FCS). Recovery methods were compared according to yield, i.e. cumulus oocyte complexes per ovary (COCs/ovary), the expansion rate (% of COCs that expanded), and nuclear maturation rate (% of …
Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, Ayhan Baştan, Bülent Polat, Duygu Baki̇ Acar, Ömer Korkmaz, Armağan Çolak
Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, Ayhan Baştan, Bülent Polat, Duygu Baki̇ Acar, Ömer Korkmaz, Armağan Çolak
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the optimal dose of EGF on in vitro maturation of bovine oocytes and to investigate the effect of different IVF and IVC media on development of bovine embryos matured with EGF. Oocytes collected from a slaughterhouse were randomly divided into 4 groups. Each group of oocytes was incubated with different doses of EGF (Control: 0 ng/mL, Group 1: 1 ng/mL, Group 2: 10 ng/mL, and Group 3: 100 ng/mL) for 24 h to determine the optimal dose. Matured oocytes were fertilized with frozen-thawed bull sperm by using the swim-up method. The oocytes …
Reorganisation Of Cell Cytoskeleton Of Cat Oocytes Matured In Vitro, Theerawat Tharasanit, Mongkol Techakumphu, Chainarong Lohachit
Reorganisation Of Cell Cytoskeleton Of Cat Oocytes Matured In Vitro, Theerawat Tharasanit, Mongkol Techakumphu, Chainarong Lohachit
The Thai Journal of Veterinary Medicine
Developmental capacity of domestic cat oocyte matured in vitro is poor when compared to that of in vivo matured oocyte. While cytoplasmic maturation of the oocytes is essentially required for embryo development, little is really known about distribution of cell components during the course of in vitro maturation (IVM). The aims of this study were to examine and to describe the changes in the distribution of microtubule and actin microfilaments in relation to stage of nuclear maturation during IVM. Cumulus oocyte complexes were matured in vitro for 0, 6, 12, 18 and 24 h and then labeled with immunofluorescent dyes …
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 100, and 1000 ng/ml EGF groups, as well as positive (EGF replaced with hCG) and negative (TCM 199 only, no supplement) control groups. Maturation and fertilisation rates were calculated at 39 °C in an atmosphere of …
Gonadal Oocyte Development In Lhrha Hormone Treated European Sea Bass (Dicentrarchus Labrax L., 1758) Broodstock, Kürşat Firat, Şahi̇n Saka, Cüneyt Süzer
Gonadal Oocyte Development In Lhrha Hormone Treated European Sea Bass (Dicentrarchus Labrax L., 1758) Broodstock, Kürşat Firat, Şahi̇n Saka, Cüneyt Süzer
Turkish Journal of Veterinary & Animal Sciences
Stages of oocyte maturation in sea bass (Dicentrarchus labrax) gonads after LHRHa hormonal treatment were investigated. LHRHa was injected into sea bass broodstock with an oocyte diameter of 527-580 µm. A second injection was given 12 h after the first injection. For each injection a dose of 10 µg/kg was used. At 24 h, oil globules in oocytes were randomly distributed. These oil globules started to fuse and change to a spherical shape from an ellipsoidal shape at 36 and 42 h. Ovulation was observed between 45 and 54 h after the first intramuscular injection. After 54 h from the …
Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu
Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objective of the present study was to investigate the ultrastructural changes seen in frozen porcine oocytes at the germinal vesicle and during in vitro maturation when using an ultrarapid freezing technique. The morphological changes were assessed by transmission electron microscopy (TEM). Immature oocytes were collected from pig ovaries at a local slaughter house in Nakorn Prathom province. Some oocytes were matured in vitro for 44 hours at 39oC, 5% CO2 with high humidity. All oocytes were divided into four groups; immature (control group), the in vitro maturation group (control group), frozen immature and frozen mature oocytes. Electron microscopy was …
Developing Embryo Technologies For The Eland Antelope (Taurotragus Oryx), Gemechu G. Wirtu
Developing Embryo Technologies For The Eland Antelope (Taurotragus Oryx), Gemechu G. Wirtu
LSU Doctoral Dissertations
Assisted reproductive technologies developed in domestic cattle serve as a starting point in similar studies on nondomestic bovids. The common eland is a useful model species for studies on rare tragelaphine antelopes. In Chapter 3 of the present study, effects of components/attributes of protein-free embryo culture media on the in vitro development of in vitro-derived bovine embryos were evaluated. A 2 x 2 factorial study comparing effects of groups of amino acids (20aa or 11aa) in two base media (modified KSOM or BM-3) demonstrated that amino acids and base medium affected embryonic development. A subsequent 7 x 2 factorial experiment …
Studies On The In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Hatem Atalla, Sema Bi̇rler, Alper Baran, Kemal Ak
Studies On The In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Hatem Atalla, Sema Bi̇rler, Alper Baran, Kemal Ak
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin (BSA) supplemented Synthetic Oviduct Fluid (SOF) and Ham's F-10 media on the in vitro maturation of cat oocytes. Oocytes collected from spayed stray queens served as the material of the study. The ovaries were brought to the laboratory within 2 h in PBS solution at 38 °C. Recovered oocytes were divided into two groups (Group 1: SOF + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH, group 2: Ham's F-10 + 0,4% BSA + 10 µg/ml FSH + 10 µg/ml LH) …
Superovulation Responses In Rabbits To Different Doses Of Follicle Stimulating Hormone, Mongkol Techakumphu, Pranee Numchaisrika, Vaneda Suktrakun, Suthai Phanitkitcharoen, Astasit Kaewnanuer, Somchai Suwajanakorn
Superovulation Responses In Rabbits To Different Doses Of Follicle Stimulating Hormone, Mongkol Techakumphu, Pranee Numchaisrika, Vaneda Suktrakun, Suthai Phanitkitcharoen, Astasit Kaewnanuer, Somchai Suwajanakorn
The Thai Journal of Veterinary Medicine
The objective of the experiment was to study the number of oocytes recovered, their quality and the recovery rate after superovulation, in order to use the oocytes, as a cytoplasm recipient in a nuclear transfer program. A total of 38, New Zealand White mature, doe rabbits were divided into 3 groups, according to the chosen dose of Follicle Stimulating Hormone (FSH); 21 mg (group A), 28 mg (group B) and 40 mg (group C). Five IM injections of FSH were given at 12 hr interval by increasing doses for group A and B and a constant dose for group C. …
The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek
The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek
Turkish Journal of Veterinary & Animal Sciences
The effects of protein sources added to the medium for maturation of bitch oocytes were investigated. Ovaries from spayed bitches were transported to the laboratory in PBS at 38 ºC within an hour. Recovered oocytes were divided into three groups and left for 72 h maturation at 38.5 ºC under an atmosphere of 5% CO_2 and high humidity. Group 1 (Control): TCM 199 medium, Group 2 (BSA): TCM 199 medium supplemented with 0.3% BSA, Group 3 (FCS): TCM 199 medium supplemented with 5% FCS. At the end of the maturation period, all oocytes were denuded from cumulus cells by pipetting …
The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek
The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23mM Na Pyruvate + FSH (Sigma F-2293) (1 µg/ml) + LH (Sigma L-5269) …
The Use Of Fresh, Salt-Stored And Frozen, Immature And Mature Oocytes, To Assess The Penetration Ability Of Porcine Spermatozoa, Adisorn Adirekthaworn, Mongkol Techakumphu, Sudson Sirivaidyapong, Saroch Gnamkum
The Use Of Fresh, Salt-Stored And Frozen, Immature And Mature Oocytes, To Assess The Penetration Ability Of Porcine Spermatozoa, Adisorn Adirekthaworn, Mongkol Techakumphu, Sudson Sirivaidyapong, Saroch Gnamkum
The Thai Journal of Veterinary Medicine
The objective of this study was to compare the penetration rate of sperm, collected from two Landrace Boars A and B, into fresh, salt-stored and frozen, immature and matured oocytes. The first experiment, performed on immature oocytes, revealed that the penetration rates for the three groups of oocytes was from Boar A 59.6%(59/99), 78.1%(75/96) and 77.8%(77/99) (p<0.05) and the number of sperm penetration each oocyte was 2.79±0.42, 2.97±0.29 and 2.29±0.26 respectively (p>0.05). While for Boar B, the percentages were 65.3%(62/95), 76.8%(73/95) and 77.8%(77/99) with the number of sperm per oocyte being 2.25±0.28, 3.63±0.42 and 2.57±0.36. The highest values were found in the salt-stored group (p<0.05). The second experiment involved sperm penetration into in vitro mature oocytes. No differences were found for the sperm penetration rate and the number of sperm per oocyte using semen from Boar A which were 85.1%(74/87), 86.1%(56/65) and 89.1%(79/88) and 13.87±1.45, 17.69±2.61 and 14.45±1.75. While for Boar B, the lowest penetration rate was found when using fresh oocyte, lower than the two other groups; 52.6%(50/95), 67.3%(64/95) and 69.1%(67/ 97)(p<0.05). The number of sperm entering each oocyte was also inferior in the fresh oocytes than in salt-stored and frozen ones; 1.55±0.31, 2.80±0.35 and 2.87±0.40 (p<0.05) respectively. The conclusion that can be drawn from the experiment is that a) there are significant differences in the sperm penetration ability of different the boars used and b) the type of oocytes; immature vs. mature and fresh vs. salt-stored vs. frozen influenced the results that were obtained. It maybe possible to apply such an in vitro penetration assay to determine boar semen fertility under field conditions.
Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol
Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol
The Thai Journal of Veterinary Medicine
Six-day embryos were recovered from 25 swamp buffalo cows treated with pregnant mare serum gonadotropin (2,500 iu PMSG, n=9) and follicle stimulating hormone (280 mg, n=12 and 400 mg FSH, n=4). Two techniques, non surgical collection from live animals and direct flushing after slaughter were compared. The oocytes were collected from ovaries immediately after slaughter by aspiration with or without ovarian chopping. The studies showed that 0.44+0.59 embryos were collected from PMSG group, while 1.38+1.31 embryos were collected in FSH group. Fifty percent to 60% of recovered embryos were transferable. The non surgical technique gave a low recovery rate (14.40%) …
The Effect Of Oocyte Preparation On Embryo Cleavage Rate After In Vitro Fertilization In The Pig, Mongkol Techakumphu, Wanpen Srianan
The Effect Of Oocyte Preparation On Embryo Cleavage Rate After In Vitro Fertilization In The Pig, Mongkol Techakumphu, Wanpen Srianan
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the optimum condition for porcine oocyte preparation for in vitro fertilization. A total of 1,130 immature oocytes collected from superficial follicles, 3-6 mm in diameter were allocated to 3 experiments. In Experiment 1, the length of time for oocyte maturation were examined (n=469). Immature oocytes were cultivated in TCM199 NaHCO3 supplemented with 10 μg/ml of FSH/LH and 1 μg/ml of Estradiol-17ẞ for 24, 40 or 48 hrs. Approximately 20% of oocytes were fixed and stained with aceto orcein to observe the maturation rate and the remainder were fertilized in vitro by freshly …