Medicine and Health Sciences Commons^™

Occurrence Of Some Pathogenic Bacteria In Cattle Feed Samples Before And After Invasionby European Starlings, Özcan Şahi̇n, Talha Demi̇rci̇, Sai̇m Boztepe, İbrahi̇m Ayteki̇n, İsmai̇l Keski̇n, Uğur Zülkadi̇r

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the species identification and enumeration of some pathogenic microorganisms before and after feeding the full ration contaminated by predatory starling bird herds on 5 different days in the dairy cattle farm in Aksaray. At the end of the study, the average number of E.coli obtained from the feed samples taken before the arrival of starlings was 6.46 log cfu/g, while the number of E.coli obtained from the feed taken after starling birds flocked to the feed was found as 6.80 log cfu/g. While the average Campylobacter spp. number was 5.50 log cfu/g, this …

Pharmacokinetics Of Tulathromycin In Pregnant Ewes (Ovis Aries) Challenged With Campylobacter Jejuni, Michael Yaeger, Jonathan P. Mochel, Zuowei Wu, Paul Plummer, Orhan Sahin, Joseph Smith, Melda Ocal, Ashenafi Beyi, Changyun Xu, Qijing Zhang, Ronald W. Griffith

Faculty Publications and Other Works -- Large Animal Clinical Sciences

The purpose of this study was to evaluate the pharmacokinetics of tulathromycin in the plasma and maternal and fetal tissues of pregnant ewes when administered within 24 hours of a single, IV Campylobacter jejuni (C. jejuni) challenge. Twelve, pregnant ewes between 72–92 days of gestation were challenged IV with C. jejuni IA3902 and then treated with 1.1 ml/45.36 kg of tulathromycin subcutaneously 18 hours post-challenge. Ewes were bled at predetermined time points and euthanized either at a predetermined time point or following the observation of vaginal bleeding or abortion. Following euthanasia, tissues were collected for bacterial …

The Status Of Antimicrobial Resistance In Campylobacterspp. Isolated From Animals And Humans In Southeast Asia: A Review, Saeid Jafari, Mahdi Ebrahimi, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Campylobacteris considered to be a major foodborne pathogen associated with human bacterial gastroenteritis in many parts of the world. Southeast Asia (SEA) has been challenging for infectious diseases and antimicrobial resistance in recent years. Antibiotic resistance in Campylobacterisolates has been reported in humans and animals in this region.Since the SEA region is one of the top tourist destinations of the world, the provision of safe food is of importance for the travel-related foodborne infections. Therefore, it is essential to elucidate the status of antibiotic resistance of Campylobacterspp. in the SEA region to reduce the impact of infection and to implement …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses, Suthida Muangnoicharoen, Sakaoporn Prachantasena, Luck Hankla, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Campylobacteriosis, one of the most common food-borne diseases in humans, is mainly caused by the consumption of chicken meat contaminated with Campylobacter. In this study, we examined the recovery rate of Campylobacter from carcass rinse using direct plating method and selective enrichment method. Campylobacter genotypes obtained from both methods were also determined using flaA-short variable region (flaA-SVR) sequencing. Results showed that Campylobacter recovery rates by the direct plating method and selective enrichment method were 40.00% and 46.86%, respectively. However, when a combination of the two methods was used, the recovery rate increased to 64.57%. For genetic characterization of Campylobacter, 9 …

Prevalence And Antimicrobial Resistance Of Salmonella And Campylobacter Species Isolated From Laying Duck Flocks In Confinement And Free-Grazing Systems, Chalermkiat Saengthongpinit, Siriporn Kongsoi, Srisamai Viriyarampa, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Prevalence and antimicrobial resistance of Salmonella and Campylobacter species isolated from duck laying flocks in 7 confinement systems and 7 free-grazing systems were determined in central Thailand. In the confinement system, 13.8% (88/639) of Campylobacter spp. were isolated from cloacal swabs; 72.7% was identified as C. jejuni and 27.3% was identified as C. coli. From drinking and surface water samples, 60.0% of Campylobacter spp. were isolated; all was identified as C. jejuni. In the free-grazing system, 0.3% (2/700) of Campylobacter spp. were isolated from cloacal swabs; all was identified as C. jejuni. In addition, 52.9% was isolated from drinking and …

Antimicrobial Resistance Patterns And Flaa Genotypes Of Campylobacter Jejuni Isolated From Contracted Broiler Farms In Eastern Thailand, Petcharatt Charununtakorn, Sakaoporn Prachantasena, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

The increasing incidence of antibiotic-resistant Campylobacter has become a major public health concern. Since little is known about antimicrobial resistance of Campylobacter isolated from contracted broiler farms, the major type of farm that produces chicken meat for domestic consumption in Thailand, the objective of the present study was to determine the antimicrobial resistance patterns and genotypes of Campylobacter isolated from contracted broiler farms where antibiotics were routinely used in their production. Sixty-nine Campylobacter jejuni isolates from cloacal swabs of chickens reared in 2 small contracted broiler farms in eastern Thailand were tested for their susceptibility to 5 antimicrobial agents by …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effects of effective microorganisms (EM) product on ammonia reduction, growth performances, Salmonella and Campylobacter reduction and hematological changes in broilers. Five hundred and ninety four day-old female Arbor Acres were divided into 3 groups with 6 replicates in each group. Group 1 was the control group, while groups 2 and 3 received EM product in forms of daily spraying at 1:50 EM solution/m2 on litter and in drinking water at 1:800 dilution, respectively. Ammonia concentrations were measured weekly, while blood samples were collected at 21 and 42 days of age for …

Socioeconomic Determinants Of Geographic Disparities In Campylobacteriosis Risk: A Comparison Of Global And Local Modeling Approaches, Jennifer Weisent, Barton Rohrbach, John Dunn, Agricola Odoi

Agricola Odoi

Background: Socioeconomic factors play a complex role in determining the risk of campylobacteriosis. Understanding the spatial interplay between these factors and disease risk can guide disease control programs. Historically, Poisson and negative binomial models have been used to investigate determinants of geographic disparities in risk. Spatial regression models, which allow modeling of spatial effects, have been used to improve these modeling efforts. Geographically weighted regression (GWR) takes this a step further by estimating local regression coefficients, thereby allowing estimations of associations that vary in space. These recent approaches increase our understanding of how geography influences the associations between determinants and …

Socioeconomic Determinants Of Geographic Disparities In Campylobacteriosis Risk: A Comparison Of Global And Local Modeling Approaches, Jennifer Weisent, Barton Rohrbach, John R. Dunn, Agricola Odoi

Animal Science Publications and Other Works

Background

Socioeconomic factors play a complex role in determining the risk of campylobacteriosis. Understanding the spatial interplay between these factors and disease risk can guide disease control programs. Historically, Poisson and negative binomial models have been used to investigate determinants of geographic disparities in risk. Spatial regression models, which allow modeling of spatial effects, have been used to improve these modeling efforts. Geographically weighted regression (GWR) takes this a step further by estimating local regression coefficients, thereby allowing estimations of associations that vary in space. These recent approaches increase our understanding of how geography influences the associations between determinants and …

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Transgenesis Collection

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

Emergence Of Quinolone Resistance Among Chicken Isolates Of Campylobacter In Turkey, Serap Savaşan, Alper Çi̇ftçi̇, K. Serdar Di̇ker

Turkish Journal of Veterinary & Animal Sciences

The development and extent of quinolone-resistance in broiler isolates of campylobacters over a 15-year period was investigated. Minimal inhibitory concentrations of enrofloxacin, ciprofloxacin and nalidixic acid for 567 Campylobacter jejuni and 233 Campylobacter coli strains were determined by the agar dilution method. Of the strains, 180, 420 and 200 were isolated during 1987, 1992 and 2000, respectively. Enrofloxacin or ciprofloxacin resistance was not found in isolates from 1987. The first fluoroquinolone resistance (1.4% to enrofloxacin and 1.2% to ciprofloxacin) appeared in strains isolated in 1992, approximately 2 years after the licensing of enrofloxacin in Turkey. The rate of resistance to …

Immunoblot Analysis Of Campylobacters Of Chickens, Mehmet Akan, Jale Erdeğer, Zi̇ya İlhan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine immunogenic structures by immunoblotting in thermophilic Campylobacter strains isolated from chickens. The bands obtained from C. jejuni strains were found to be distributed between 10.8 and 116.5 kD by SDS-PAGE analysis of whole cell proteins. The bands 78, 63, 52, 48.5, 43.5, 29.8, 25.6, 22, 18.5, 17.2 and 16.5 kD were detected as the major common bands in all C. jejuni strains. Electrophoretic analysis of C. coli strains revealed that protein band profiles ranged between 10.3 and 118 kD, and 76, 63-62, 52, 48.5, 44 and 30.5 kD bands were all major …

Colonization, Translocation And Antibody Response In Experimental Campylobacter Infection Of Chickens, Hakan Yardimci, Jale Erdeğer, Mehmet Akan, Murat Yildirim

Turkish Journal of Veterinary & Animal Sciences

In this study we examined factors affecting the colonization of campylobacters in chick intestines, colonization times and elicited immune responses. In chicks, infected orally by minimal infective dose _{100} (MID_{100}), colonization in the intestines was observed to reach 100% during the 3-week period following C. jejuni and C. coli inoculation. When the SDS-PAGE protein profiles of the inoculated and isolated strains were examined similarities of 98.3% among C. jejuni strains and 98.5% among C. coli strains were determined. The results obtained from the ELISA tests performed with blood sera in the group infected with C. jejuni and in the control …