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Full-Text Articles in Medicine and Health Sciences

Determining The Effects Of Different Bypass Fat Supplementation On Performance, Milkyield, And Milk Composition Of Anatolian Buffaloes, Sezer Öz, Seher Küçükersan Jan 2024

Determining The Effects Of Different Bypass Fat Supplementation On Performance, Milkyield, And Milk Composition Of Anatolian Buffaloes, Sezer Öz, Seher Küçükersan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to explore the effects of animal bypass fat (ABF) and palm bypass fat (PBF) added to the rations of Anatolianbuffaloes on dry matter consumption, feed conversion ratio, milk yield, some milk components, and parameters. A total of 21 heads ofbuffalo in the early lactation period; milk yield averages, lactation periods, lactation numbers, live weights, and ages were selected anddivided into three experimental groups, each consisting of 7 buffaloes. The experiment was conducted according to the crossover trialpattern. There was no statistically significant difference between the groups in terms of dry matter consumption and feed conversionratio (p > 0.05). …


Investigation Of Three Single-Nucleotide Polymorphisms (1252 G>A, 2534 G>A, And 2569 T>C) Of The Mbl1 Gene In Anatolian Buffaloes, Esma Gamze Aksel, Bi̇lal Akyüz Dec 2023

Investigation Of Three Single-Nucleotide Polymorphisms (1252 G>A, 2534 G>A, And 2569 T>C) Of The Mbl1 Gene In Anatolian Buffaloes, Esma Gamze Aksel, Bi̇lal Akyüz

Turkish Journal of Veterinary & Animal Sciences

It has been reported that since the MBL gene is involved in the first step of infection and inflammation processes, it may be associated with susceptibility to bacterial and viral diseases in living organisms. In this study, it was aimed to determine the genotypic variation of Anatolian buffaloes in terms of three (1252G>A, 2534 G>A, and 2569 T>C) single nucleotide polymorphisms (SNPs) within the MBL-1 gene. For this purpose, 103 Anatolian buffaloes were genotyped for relevant SNPs by PCR-RFLP process. The allele and genotype frequencies of the examined samples were determined, and the population was examined for …


Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron Aug 2023

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …


Genotyping Of The Κ - Casein Gene By Pcr - Rflp (Acui Digestion) And Its Relationship To Milk Yield Traits In Anatolian Buffaloes, Murrah Buffaloes, And Their Crosses, Orhan Karadağ, Vasfi̇ye Kader Esen, Mehmet Aki̇f Yüksel, Mustafa Küçükkebabçi, Semi̇h Sevi̇m, İlkay Çeti̇n, Bi̇nnur Kaptan, Mehmet İhsan Soysal Jan 2023

Genotyping Of The Κ - Casein Gene By Pcr - Rflp (Acui Digestion) And Its Relationship To Milk Yield Traits In Anatolian Buffaloes, Murrah Buffaloes, And Their Crosses, Orhan Karadağ, Vasfi̇ye Kader Esen, Mehmet Aki̇f Yüksel, Mustafa Küçükkebabçi, Semi̇h Sevi̇m, İlkay Çeti̇n, Bi̇nnur Kaptan, Mehmet İhsan Soysal

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the polymorphism of the κ - casein gene (kappa casein, CSN3) in buffaloes and determine its effects on milk yield traits. Data were obtained from 2016 to 2019 from all animals in the ongoing Anatolian Buffalo Breeding Project. Genomic DNA was collected from a total of 209 buffaloes of different ages and sexes, namely 31 Murrah, 46 Anatolian buffaloes, and 132 of their crosses (Murrah × Anatolian). Genotyping of CSN3 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) technique with restriction enzyme (AcuI) revealed three …


A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir Jan 2023

A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir

Turkish Journal of Veterinary & Animal Sciences

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …


Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs Dec 2022

Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs

The Thai Journal of Veterinary Medicine

Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations in freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify the fundamental differences between high (HF) and low freezability (LF) ejaculated spermatozoa in swamp buffaloes, which were limitedly described for this agricultural species. Twelve (12) different samples of bubaline semen were collected, cryopreserved and categorized into freezability phenotypes using post-thaw sperm motility as the basis. Sperm kinematics and functional plasma membrane integrity were evaluated in HF and LF samples using computer-assisted sperm analysis (CASA) and hypo-osmotic swelling test, respectively. The relative concentrations of some …


Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas Dec 2021

Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas

The Thai Journal of Veterinary Medicine

Shiga toxin-producing Escherichia coli (STEC) is an important causal agent of food borne diseases. Although the presence of STEC has been reported in cattle, goat, and sheep in previous studies in Bangladesh, there is seemingly no information on sorbitol non-fermenting STEC (SN-F STEC) carriage in buffalo. We aimed to assess the prevalence of SN-F STEC and to identify common virulence genes in SN-F STEC strains from apparently healthy buffaloes on smallholdings in coastal area in Bangladesh. Total 308 buffaloes on smallholdings in Patenga and Anwara Upazila, Chattogram division, Bangladesh were randomly sampled by collecting a swab from the recto-anal junction …


Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya Dec 2021

Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya

The Thai Journal of Veterinary Medicine

Application proper procedures for thawing of cryopreserved sperm are required for successful artificial insemination. The study was aimed to compare the effects of watery and dry thawing systems on motility, acrosome integrity, plasma membrane integrity, morphologic and kinematic characteristics of buffalo bull spermatozoa. Anatolian Buffalo bull spermatozoa, cryopreserved in volumes of 0.25 mL straws, were thawed by using 1) watery thawing system; a total of 10 straws were thawed in a water bath at 37 °C for 30 sec and 2) dry thawing system; a total of 10 straws were thawed in a dry thawing device at 37 °C for …


Associations Of Snps In Ghr Gene With Growth And Milk Yield Of Anatolian Buffaloes, Meti̇n Erdoğan, Mustafa Tekerli̇, Koray Çeli̇keloğlu, Özlem Hacan, Serdar Koçak, Zehra Bozkurt, Samet Çi̇nkaya, Mustafa Demi̇rtaş Jan 2021

Associations Of Snps In Ghr Gene With Growth And Milk Yield Of Anatolian Buffaloes, Meti̇n Erdoğan, Mustafa Tekerli̇, Koray Çeli̇keloğlu, Özlem Hacan, Serdar Koçak, Zehra Bozkurt, Samet Çi̇nkaya, Mustafa Demi̇rtaş

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the associations between single nucleotide polymorphisms (SNP) in the growth hormone receptor (GHR) gene and growth and milk yields of Anatolian buffaloes. Growth records of genotyped 3012 Anatolian buffaloes and 467 lactations of 329 cows from them were used. The overall means of weights at birth, six and 12 months of ages and lactation milk yield were 29.377 ± 0.342 kg, 96.15 ± 1.48 kg, 165.54 ± 2.18 kg, and 976.3 ± 39.9 kg, respectively. Three mutations (g.31601787 G > A, g.31601784 G > A, and g.31601783 G > del) in GHR were detected. A least-squares …


Effect Of Chemical- And Toxin Binder-Treated Cotton Seed Cake On Milk Production, Milkcomposition, And Aflatoxin Concentration In Milk Of Nili Ravi Lactating Buffaloes, Murtaza Ali Tipu, Anjum Khalique, Muhammad Naveed Ul-Haque, Muhammad Qamer Shahid, Maqsood Akhtar, Mubashir Ilyas Jan 2020

Effect Of Chemical- And Toxin Binder-Treated Cotton Seed Cake On Milk Production, Milkcomposition, And Aflatoxin Concentration In Milk Of Nili Ravi Lactating Buffaloes, Murtaza Ali Tipu, Anjum Khalique, Muhammad Naveed Ul-Haque, Muhammad Qamer Shahid, Maqsood Akhtar, Mubashir Ilyas

Turkish Journal of Veterinary & Animal Sciences

Twelve multiparous Nili Ravi buffaloes were assigned to 1 of 3 treatments in a randomized, replicated 3 × 3 Latin square design to evaluate the effect of chemical (calcium propionate)- and toxin binder (sodium bentonite)-treated cotton seed cake (CSC) on milk yield and transfer of milk toxin (M1). Periods (20 days) were allocated for each treatment. In the 1st treatment (control), CSC with 100 μg/kg of aflatoxin B1 (AFB1) was fed to buffaloes. The 2nd and 3rd treatments were 0.5% calcium-propionate-treated CSC and 1.0% sodium-bentonite-treated CSC, respectively. Each group had 4 multiparous buffaloes. The buffaloes given 0.5% calcium propionate CSC …


Seasonal Variations In Histomorphology And Histochemistry Of The Prostate Gland Ofbuffalo Bulls, Samikshya Sarangi, Anuradha Gupta, Neelam Bansal, Varinder Uppal Jan 2020

Seasonal Variations In Histomorphology And Histochemistry Of The Prostate Gland Ofbuffalo Bulls, Samikshya Sarangi, Anuradha Gupta, Neelam Bansal, Varinder Uppal

Turkish Journal of Veterinary & Animal Sciences

The prostate glands of buffalo bulls were collected from an abattoir immediately after slaughter and were fixed in 10% neutral buffered formalin for paraffin block preparation by an acetone-benzene schedule. Paraffin sections of 3-5 μm were obtained on glass slides with a rotary microtome and were stained with various histomorphological and histochemical stains. The distributions of glandular acini and ducts were variable throughout the length of the pelvic urethra. The disseminate part of the prostate gland was derived from the epithelium of the pelvic urethra and was a compound tubulo-alveolar type of gland. The glandular acini comprised three types of …


Biochemical And Physiological Responses Of Nili-Ravi Buffalo (Bubalus Bubalis) To Heatstress, Umair Younas, Muhammad Abdullah, Jalees Ahmed Bhatti, Nisar Ahmed, Faisal Shahzad, Musadiq Idris, Sana Tehseen, Muhammad Junaid, Saima Tehseen, Saeed Ahmed Jan 2020

Biochemical And Physiological Responses Of Nili-Ravi Buffalo (Bubalus Bubalis) To Heatstress, Umair Younas, Muhammad Abdullah, Jalees Ahmed Bhatti, Nisar Ahmed, Faisal Shahzad, Musadiq Idris, Sana Tehseen, Muhammad Junaid, Saima Tehseen, Saeed Ahmed

Turkish Journal of Veterinary & Animal Sciences

The present study was designed with an objective to evaluate the impact of various strategies to alleviate heat stress on certain physiological attributes viz. respiratory rate (RT), body surface temperature (BST), pulse rate (PR), and respiratory rate (RR); biochemical attributes viz. glucose, total protein (TP), and cholesterol; and endocrinological parameters (tri-iodothyronine and tetraiodothyronine) of the Nili-Ravi buffaloes during early summer season. Lactating Nili-Ravi buffaloes (n = 20) with close production and parity stage were divided into four treatment groups as group A (control group under roof shades only), B (experimental group under roof shades with antistress supplementation), C (experimental group …


Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal Jan 2019

Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal

Turkish Journal of Veterinary & Animal Sciences

In this study, we established the components of the buffalo spermatogonial stem cell (SSC) niche in prenatal (38.5-280 days of gestation), neonatal, prepubertal, and adult testes histochemically and immunohistochemically. Immunohistochemically, the changes in vimentin expression were evaluated. During prenatal life, the niche of primordial germ cells was formed by the surrounding mesenchymal cells and fibroblasts. The basement membrane (BM) was not organized at this stage. At 7.6 cm crown-rump length, testicular cords with a thin BM were seen. At the late gestational period, testicular cords with well-organized BM were seen. At the neonatal stage, the testicular cords were lined with …


Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts, Babu Lal Jangir, Raj Bahadur Bind, Pawan Kumar, Ramesh Somvanshi Jan 2017

Pathological Studies And Detection Of Different Bovine Papilloma Virus Types In Buffalocutaneous Warts, Babu Lal Jangir, Raj Bahadur Bind, Pawan Kumar, Ramesh Somvanshi

Turkish Journal of Veterinary & Animal Sciences

Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus (BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphology and association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which revealed that growths were either single or multiple, variable in shape and size, and present at different body sites. Histopathologically, these were categorized as papilloma, fibropapilloma, and fibroma. Polymerase chain reaction (PCR) revealed the presence of …


Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh Jan 2017

Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to look at four ultrasonographic approaches that diagnose acquired reticular diaphragmatic hernia (RDH) using either left or right caudal thoracic approaches (4th/5th intercostal space) and compare them to those made more cranially (1st/2nd intercostal space). Each sonographic observation was made in 27 unsedated, standing animals (22 buffaloes and 5 cattle) that were surgically confirmed for RDH. Using the right caudal thoracic approach, the herniated reticulum was seen as medial to the thoracic wall at a depth of 3-5 cm and adjacent to the cardiac silhouette. With the left caudal thoracic approach, the herniated reticulum …


Evidence Of Foot-And-Mouth Disease Virus Excretion In The Milk Ofapparently Healthy Vaccinated Buffaloes In Islamabad, Pakistan, Hafiziftikhar Ahmed, Umer Farooq, Aamer Bin Zahur, Khalid Naeem, Asma Latif, Hamid Irshad Jan 2017

Evidence Of Foot-And-Mouth Disease Virus Excretion In The Milk Ofapparently Healthy Vaccinated Buffaloes In Islamabad, Pakistan, Hafiziftikhar Ahmed, Umer Farooq, Aamer Bin Zahur, Khalid Naeem, Asma Latif, Hamid Irshad

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples (n = 200) were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD (78%; 156/200) in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% (19/156) of the …


Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong Sep 2016

Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong

The Thai Journal of Veterinary Medicine

Our previous study reported the expression of kisspeptin in the preoptic area (POA) and arcuate nucleus (ARC) of the hypothalamus of cycling buffalo cows. In ruminants, POA and ARC are the main hypothalamic nuclei through which kisspeptin acts on the hypothalamic-pituitary-gonadal axis, due to their stimulation of gonadotropin releasing hormone (GnRH) secretion. In many species, kisspeptin activates GnRH neurons via its receptors (KISS1R or GPR54). The present study was designed to explore the expression of KISS1R by GnRH neurons in the POA and ARC hypothalamic nuclei in buffalo cow brains by double-labeling immunohistochemistry. In both the POA and ARC areas, …


Dimensional Characteristics Of Spermatozoa Of Nili-Ravi Buffalo Bulls: Seasonaland Climatic Influences, Sajid Hameed, Saima Masood, Hafsa Zaneb, Muhammad Sarwar Khan, Muhammad Younis, Muhammad Avais, Muti Ur Rehman Khan Jan 2016

Dimensional Characteristics Of Spermatozoa Of Nili-Ravi Buffalo Bulls: Seasonaland Climatic Influences, Sajid Hameed, Saima Masood, Hafsa Zaneb, Muhammad Sarwar Khan, Muhammad Younis, Muhammad Avais, Muti Ur Rehman Khan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in 2 different climatic zones of Punjab Province of Pakistan, the Sahiwal and Bahawalpur divisions, simultaneously for the period of 1 year divided into seasons. Seven adult Nili-Ravi buffalo breeding bulls from each division were used. Dimensional characteristics of spermatozoa were determined. Season had a nonsignificant effect on the sperm head length in both divisions and a nonsignificant difference was also observed between bulls of the Sahiwal and Bahawalpur divisions. The buffalo bulls of both divisions exhibited significantly increased sperm head breadth in autumn, whereas a nonsignificant difference was observed between divisions. Sperm head shape values …


Pancreas Morphology And Duct System Of Swamp Buffaloes (Bubalus Bubalis), Tilladit Rung-Ruangkijkrai, Wuthichai Klomkleaw Jun 2014

Pancreas Morphology And Duct System Of Swamp Buffaloes (Bubalus Bubalis), Tilladit Rung-Ruangkijkrai, Wuthichai Klomkleaw

The Thai Journal of Veterinary Medicine

Swamp buffaloes have been important to Thai countryfolk for centuries. However, it’s meat consumption has increased continuously. Since meat and offal of cattle are very similar, pancreas might be used to differentiate buffalo offal from cow offal. Pancreases from 10 adult buffaloes of both sexes were obtained and studied anatomically. Studies revealed that, unlike human and other animals, buffalo pancreas had a unique F-shape with left lobe, right lobe, body, and uncinate process. There was no main pancreatic duct. Only one accessory pancreatic duct was found open into minor duodenal papilla similar to those of cow. Furthermore, unlike human and …


Genetic Characterization Of Paramphistomes Of Buffalo By Hat-Rapd Analysis, Giregowdanahalli Puttalakshmamma, Umed Ramani, Krishna Singh, Amrutlal Patel, Abdul Patel, Chaitanya Joshi Jan 2014

Genetic Characterization Of Paramphistomes Of Buffalo By Hat-Rapd Analysis, Giregowdanahalli Puttalakshmamma, Umed Ramani, Krishna Singh, Amrutlal Patel, Abdul Patel, Chaitanya Joshi

Turkish Journal of Veterinary & Animal Sciences

Paramphistomosis is widely prevalent in domestic farm animals, incurring heavy economic losses in several countries across the world. As the identification of these parasites based on morphological features is very difficult, molecular characterization is necessary to discriminate among different species. We performed polymorphic DNA fingerprint analysis of 3 different species of amphistomes collected from the rumen and bile ducts of buffaloes slaughtered at a local abattoir in Anand, Gujarat, India. The ruminal amphistomes were identified as Paramphistomum cervi and Gastrothylax indicus, while the hepatic amphistomes were identified as Gigantocotyle bathycotyle. High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis with 4 …


Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh Jan 2014

Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh

Turkish Journal of Veterinary & Animal Sciences

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (


Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes, Lakshman Sahoo, Suresh K. Singla Jan 2013

Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes, Lakshman Sahoo, Suresh K. Singla

Turkish Journal of Veterinary & Animal Sciences

Recovery rate and quality of oocytes were investigated from abattoir collected ovaries with and without corpora lutea (CL). The oocytes were categorized into four grades: grade-A, grade-B, grade-C, and grade-D based on the presence of the cumulus cells complex around the oocytes. The number of oocytes per ovary were 1.7 ±0.1 and 1.5 ±0.1, obtained from ovaries without CL and with CL, respectively. Recovery rate of different grades of oocytes from ovaries with CL and without CL were almost identical.


Ultrasonographic Evaluation Of Buffalo Eyes, Gholamreza Assadnassab, Majid Fartashvand Jan 2013

Ultrasonographic Evaluation Of Buffalo Eyes, Gholamreza Assadnassab, Majid Fartashvand

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to describe the ultrasonography appearance and measurements of normal buffalo eyes. Ocular ultrasonographic examinations were performed on 20 adult buffaloes. Transpalpebral ultrasonographic images were obtained with a 6-8 MHz linear transducer in the axial imaging plane. The ultrasonographic appearance of structures within the buffalo eye is similar to that in other species. When comparing measurements, the anteroposterior depth of the lens, vitreous chamber, and axial length of the globe on the left side were greater than on right, and the anterior chamber depth and scleroretinal rim wall thickness on the left side were also …


Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan Sep 2011

Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan

The Thai Journal of Veterinary Medicine

Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …


In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi Jan 2011

In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi

Turkish Journal of Veterinary & Animal Sciences

Conditions for in vitro maturation (IVM) of primary oocytes without conventionally used fetal calf serum and hormones were optimized in order to reduce the cost of laboratory produced buffalo embryos. Comparisons were made between oocyte recovery methods (aspiration vs. slicing) and IVM in medium 199 (static culture method vs. flux culture method) supplemented with 4-5 × 106 granulosa cells mL-1 that contained either estrus buffalo serum (EBS) or fetal calf serum (FCS). Recovery methods were compared according to yield, i.e. cumulus oocyte complexes per ovary (COCs/ovary), the expansion rate (% of COCs that expanded), and nuclear maturation rate (% of …


Prevalence Of Post Parturient Haemoglobinuria In Buffalo And Therapeutic Trials With Toldimfos Sodium And Tea Leaves In Pakistan, Aneela Zameer Durrani, Nadeem Kamal, Abdul Rauf Shakoori, Rana Muhammad Younus Jan 2010

Prevalence Of Post Parturient Haemoglobinuria In Buffalo And Therapeutic Trials With Toldimfos Sodium And Tea Leaves In Pakistan, Aneela Zameer Durrani, Nadeem Kamal, Abdul Rauf Shakoori, Rana Muhammad Younus

Turkish Journal of Veterinary & Animal Sciences

The present study was carried out to investigate the prevalence of post parturient haemoglobinuria in buffaloes, and to compare the therapeutic efficacy of toldimfos sodium, sodium acid phosphate, and tea leaves. The prevalence of disease in 1000 animals, grouped according to varying stages of lactation and pregnancy, was recorded. Screening of positive animals based on clinical signs of haemoglobinuria, anaemia, and moderate pyrexia was performed. Urine and blood samples were collected. Blood samples were processed for haematological parameters and biochemical profile. Blood films were made and stained. The animals that were positive for disease were treated with sodium acid phosphate, …


Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong Jun 2009

Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong

The Thai Journal of Veterinary Medicine

The present study aimed to examine the general morphology of Thai swamp buffalo’s oviducts by light microscopy (LM) and scanning electron microscopy (SEM) as well as the expression of estrogen receptor alpha (ERα) and progesterone receptor isoform B (PRB) by immunohistochemical technique at the prepubertal stage. The samples composed of the uterotubal junction (UTJ), isthmus, ampulla and infundibulum of prepubertal Thai swamp buffalo oviducts (n=6) were collected from a local slaughterhouse. The results showed that the epithelium of prepubertal Thai swamp buffalo oviducts was composed of ciliated and secretory cells but the feature of nuclear/cytoplasmic protrusions of secretory cells was …


An Evaluation Of Subclinical Mastitis During Lactation In Anatolian Buffaloes, Erhan Özenç, Mehmet Rifat Vural, Esra Şeker, Mehmet Uçar Jan 2008

An Evaluation Of Subclinical Mastitis During Lactation In Anatolian Buffaloes, Erhan Özenç, Mehmet Rifat Vural, Esra Şeker, Mehmet Uçar

Turkish Journal of Veterinary & Animal Sciences

Subclinical mastitis in Anatolian buffaloes (n = 71) in Afyon, Turkey was evaluated using 1637 milk samples collected monthly for 9 months. Tests applied to milk samples included the California Mastitis Test (CMT), somatic cell count (SCC), and bacteriological examination. The threshold limit for SCC was found as 130 × 10^3 cells/ml. Anatolian buffaloes are infected above this level and they are likely to be uninfected below this level. The highest rates of intramammary infection (IMI) per quarter (36.5%) and animal (69.1%) were encountered during the highest rainfall month of May. Candida spp., coagulase-negative staphylococcus (CNS) and Staphylococcus aureus and …


Harvesting And Evaluation Of Riverine Buffalo Follicular Oocytes, Huma Jamil, Hafiz Abdus Samad, Zafar Iqbal Qureshi, Najeeb Ur Rehman, Laeed Akbar Lodhi Jan 2008

Harvesting And Evaluation Of Riverine Buffalo Follicular Oocytes, Huma Jamil, Hafiz Abdus Samad, Zafar Iqbal Qureshi, Najeeb Ur Rehman, Laeed Akbar Lodhi

Turkish Journal of Veterinary & Animal Sciences

The low superovulatory response and poor recovery rate of oocytes are major impediments to the application of in vitro fertilization and embryo transfer technology in buffaloes. The present study aimed to evaluate the comparative efficacy of oocyte collection methods, season (low and peak breeding season), and ovarian status (presence or absence of corpus luteum) on the recovery rate and quality of recovered buffalo follicular oocytes. For this purpose riverine buffalo ovaries were collected from buffaloes slaughtered at Faisalabad Municipal Corporation Slaughter House. To study the efficiency of 3 different recovery methods the ovaries were dissected (n = 291), punctured (n …


Influence Of Bovine Somatotropin And Replacement Of Corn Dextrose With Concentrate On The Performance Of Mid-Lactating Buffaloes Fed Urea-Treated Wheat Straw, Muhammad Sarwar, Muhammad Asif Shahzad, Mahr-Un Nisa, Aboubakar Sufyan Jan 2007

Influence Of Bovine Somatotropin And Replacement Of Corn Dextrose With Concentrate On The Performance Of Mid-Lactating Buffaloes Fed Urea-Treated Wheat Straw, Muhammad Sarwar, Muhammad Asif Shahzad, Mahr-Un Nisa, Aboubakar Sufyan

Turkish Journal of Veterinary & Animal Sciences

The study examined the effect of bovine somatotropin (bST) in mid-lactating Nili-Ravi buffaloes (n = 18) given varying levels of Enzose (corn dextrose) in a urea-treated wheat straw (UTWS) based ration on nutrient intake, digestibility, nitrogen (N) utilisation, milk yield, and its composition in a completely randomised design with a 2 x 3 factorial arrangement. The control ration contained 60% UTWS and 40% concentrate. The other 2 rations were formulated to replace 50% or 100% concentrate portions of the ration with Enzose of equivalent energy. Enzose is a liquid derived from the enzymatic conversion of corn starch to dextrose, and …