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Full-Text Articles in Medicine and Health Sciences
Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong
Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong
Master's Theses
Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …
Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend
Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend
Master's Theses
Raccoons (Procyon lotor) serve as a source of infection with various pathogens for people, most notably, rabies. Raccoons are specifically targeted for the control of rabies in the United States, primarily due to the 100% fatality rate in humans and animals. There is no cure or treatment for rabies and vaccines may be administered to people if it is administered promptly. In order to evaluate how successful trapping of raccoons is by using conventional methods, motion sensing cameras were placed on traps in order to captured raccoon behaviors. Pictures were coded based on these behaviors (around the trap but not …