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Full-Text Articles in Medicine and Health Sciences

Acute And Subacute Pulmonary Effects Of Diesel Exhaust Particles In Mice: Pathological Changes And Translocation Pathways To The Circulation, Theerayuth Kaewamatawong, Akinori Shimada, Takehito Morita, Wijit Banlunara, Anong Bintvihok Dec 2009

Acute And Subacute Pulmonary Effects Of Diesel Exhaust Particles In Mice: Pathological Changes And Translocation Pathways To The Circulation, Theerayuth Kaewamatawong, Akinori Shimada, Takehito Morita, Wijit Banlunara, Anong Bintvihok

The Thai Journal of Veterinary Medicine

To study the acute and subacute pulmonary effects of diesel exhaust particles (DEPs), mice were intratracheally instilled with 25, 50 or 100 μg of DEPs for dose response experiments. Histological alterations were determined at 3 days post-exposure. 50 or 100 μg of DEPs produced mild to moderate pulmonary inflammation and tissue injury characterized by infiltration of neutrophils and active alveolar macrophages (AMs), focal alveolitis and particle-laden AMs accumulation. Ultrastructural studies of treated animals showed the dissociation of basement membranes and erosion of type I alveolar epithelial cells. To investigate the time response, mice were instilled with 50 μg of DEPs …


Efficacy Of Various Strains Of Infectious Bronchitis Vaccine Against Nephropathogenic Infectious Bronchitis Virus Isolated From Chickens In Thailand, Tawatchai Pohuang, Kanlaya Chuachan, Niwat Chansiripornchai, Jiroj Sasipreeyajan Dec 2009

Efficacy Of Various Strains Of Infectious Bronchitis Vaccine Against Nephropathogenic Infectious Bronchitis Virus Isolated From Chickens In Thailand, Tawatchai Pohuang, Kanlaya Chuachan, Niwat Chansiripornchai, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

One hundred, 1 week old, female broiler chickens were divided into 5 groups, 20 chickens in each. Each group was vaccinated with a different strain of infectious bronchitis vaccine; Ma5, H120 and a local strain produced by the Department of Livestock Development for groups 3, 4 and 5, respectively. All the vaccinated chickens received intraocular vaccine at 1 week old. The chickens in groups 1 and 2 served as a negative control and a positive control group, respectively. All the chickens were weighed at 1, 4 and 6 weeks old and challenged at 4 weeks old with nephropathogenic infectious bronchitis …


The Efficacy Of Escherichia Coli Aroa-Live Vaccine In Broilers Against Avian E. Coli Serotype O78 Infection, Visut Rawiwet, Niwat Chansiripornchai Dec 2009

The Efficacy Of Escherichia Coli Aroa-Live Vaccine In Broilers Against Avian E. Coli Serotype O78 Infection, Visut Rawiwet, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The efficacy of an Escherichia coli (E. coli) aroA-live vaccine in the prevention of colibacillosis in chickens following intratracheal challenge with a virulent strain of E. coli O78 was investigated. Thirty-six, one day old broiler chickens were divided into 3 groups of 12 each. Chickens in each group were randomly divided into 2 replicates. The chickens in group 1 that were not vaccinated and challenged served as a negative control. The chickens in group 2 that were not vaccinated but received E. coli serotype O78 served as a positive control. The chickens in group 3 were vaccinated by the oral …


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2009

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Proceeding Of The 4th Asian Society Of Veterinary Pathologists (Asvp) Conference And Annual Meeting Of The Thai Association Of Veterinary Laboratory Diagnosticians (Tavld) -1, Achariya Sailasuta Dec 2009

Proceeding Of The 4th Asian Society Of Veterinary Pathologists (Asvp) Conference And Annual Meeting Of The Thai Association Of Veterinary Laboratory Diagnosticians (Tavld) -1, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep Dec 2009

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Proceeding Of The 4th Asian Society Of Veterinary Pathologists (Asvp) Conference And Annual Meeting Of The Thai Association Of Veterinary Laboratory Diagnosticians (Tavld) - 2, Achariya Sailasuta Dec 2009

Proceeding Of The 4th Asian Society Of Veterinary Pathologists (Asvp) Conference And Annual Meeting Of The Thai Association Of Veterinary Laboratory Diagnosticians (Tavld) - 2, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.


In Vitro Efficacy Of Human-Derived Probiotic, Lactobacillus Rhamnosus Against Pathogenic Bacteria In Fish And Frogs, Nopadon Pirarat, Komkiew Pinpimai, Katriya Chankow, Kotchakorn Malila, Nantrika Chansue, Waree Niyomtham, Channarong Rodkhum Dec 2009

In Vitro Efficacy Of Human-Derived Probiotic, Lactobacillus Rhamnosus Against Pathogenic Bacteria In Fish And Frogs, Nopadon Pirarat, Komkiew Pinpimai, Katriya Chankow, Kotchakorn Malila, Nantrika Chansue, Waree Niyomtham, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Probiotic supplementation is now being focused as an alternative method to control fish diseases worldwide. This study investigates the in vitro efficacy of a human-derived probiotic, Lactobacillus rhamnosus. The first results of the screening for antimicrobial activity using agar spot test and disc diffusion showed that L. rhamnosus has a broad range against twelve isolates (n=12) of Gram-positive and Gram-negative pathogenic bacteria in fish and frogs : Streptococcus iniae (n=4), Streptococcus agalactiae (n=3), Aeromonas hydrophila (n=3), Chryseobacterium indologenes (n=1) and Edwardsiella tarda (n=1). Agar spot test on killed probiotic bacteria indicated that only the metabolic product of probiotic is involved …


Detection Of Antibody Titer Against Toxoplasma Gondii In Aborted Sows And Gilts In A Swine Herd In Thailand, Padet Tummaruk, Woraporn Sukhumavasi, Sasiwimon Talummuk, Annop Kunavongkrit Dec 2009

Detection Of Antibody Titer Against Toxoplasma Gondii In Aborted Sows And Gilts In A Swine Herd In Thailand, Padet Tummaruk, Woraporn Sukhumavasi, Sasiwimon Talummuk, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

No abstract provided.


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj, Winai Chansaisakorn Dec 2009

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


The Asian Society Of Veterinary Pathologists (Asvp), Achariya Sailasuta Dec 2009

The Asian Society Of Veterinary Pathologists (Asvp), Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Dec 2009

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Effects Of Permethrin At A Prophylactic Dose For Ectoparasite Infection On Cholinesterase Activity In Dogs, Piyarat Chansiripornchai, Niwat Chansiripornchai Dec 2009

Effects Of Permethrin At A Prophylactic Dose For Ectoparasite Infection On Cholinesterase Activity In Dogs, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Effects of permethrin at a prophylactic dose for ectoparasite infection on cholinesterase (ChE) activity was evaluated. Eight healthy dogs were exposed to a spot-on formulation of permethrin on day 0. The results of serum ChE activity in all of the dogs which was measured at 24 h after exposure to permethrin significantly decreased when compared to those before exposure. However, the serum ChE activity in all the dogs which was measured at 36 and 96 h after exposure to permethrin showed no significant difference as compared to those before exposure. There were no clinical signs in all dogs throughout the …


The Indicative Influence Of Oxidative Stress On Low Milk Yields In Dairy Cattle, Witaya Suriyasathaporn, Usanee Vinitketkumnuen, Teera Chewonarin, Vena Chupia, Tanu Pinyopummintr Sep 2009

The Indicative Influence Of Oxidative Stress On Low Milk Yields In Dairy Cattle, Witaya Suriyasathaporn, Usanee Vinitketkumnuen, Teera Chewonarin, Vena Chupia, Tanu Pinyopummintr

The Thai Journal of Veterinary Medicine

The goal of this study was to identify the correlation between milk yield and milk malondialdehyde (MDA), an oxidative stress marker. The study was conducted using cows from eight small-holder dairy farms during October to December 2004. The data comprised of sample date, milk yield, and the recent calving date were recorded. Morning milk samples were collected monthly for the measurement of somatic cell counts (SCC) and MDA level using the automate counter and the modified Smith’s method, respectively. The final data set included 131 milk data from 74 cows. Overall means and SEM of milk yields, somatic cell scores …


In Vitro Antibiotic Susceptibility Of Aeromonas Hydrophila Isolated From Disease Ornamental Fish, Malinee Jongjareanjai, Nongnut Assawawongkasem, Nantarika Chansue Sep 2009

In Vitro Antibiotic Susceptibility Of Aeromonas Hydrophila Isolated From Disease Ornamental Fish, Malinee Jongjareanjai, Nongnut Assawawongkasem, Nantarika Chansue

The Thai Journal of Veterinary Medicine

Thirty bacterial isolates were derived from ornamental fish patients (n=30) of the Veterinary Medical Aquatic Animal Research Center (VMARC), Faculty of Veterinary Science, Chulalongkorn University. The isolated bacterial strains were identified by using commercial biochemical identification kit (API Kits, bioMerieux® SA France). The antibiotics susceptibility testing was performed by disc diffusion method, using 24 types of antibiotic discs (OXOID, Oxoid Ltd, UK). The result showed that the majority of the isolated bacteria was Aeromonas hydrophila (27/30). In the A. hydrophila susceptibility study, 66.30% of bacteria were antibiotic resistant, 9.35% were intermediately sensitive and 24.35% were sensitive strains against the tested …


Growth Characteristics Of The H5n1 Avian Influenza Virus In Chicken Embryonic Eggs And Mdck Cells, Wisanu Wanasawaeng, Napawan Bunpapong, Wichet Leelamanit, Roongroje Thanawongnuwech Sep 2009

Growth Characteristics Of The H5n1 Avian Influenza Virus In Chicken Embryonic Eggs And Mdck Cells, Wisanu Wanasawaeng, Napawan Bunpapong, Wichet Leelamanit, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Chicken embryonic eggs and Madin-Darby canine kidney (MDCK) cell line were used for comparing the propagation of avian influenza A (H5N1) virus (C2105Dx1, a Thai isolate). The growth of the H5N1 virus was determined by using HA test and 50% infectivity dose to assess the suitability of the systems supporting the propagation of the virus. The results indicated that the Thai H5N1 virus was propagated better in the chicken embryonic eggs, which should be considered as a system of choice for the avian H5N1 virus isolation. The optimal time for harvesting the selected Thai H5N1 virus was at 24 hours …


Ultrasound Diagnosis, Phiwipha Kamonrat Sep 2009

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Effects Of Medium Chain Fatty Acids, Organic Acids And Fructo-Oligosaccharide On Cecal Salmonella Enteritidis Colonization And Intestinal Parameters Of Broilers, Sucheera Chotikatum, Indhira Kramomthong, Kris Angkanaporn Sep 2009

Effects Of Medium Chain Fatty Acids, Organic Acids And Fructo-Oligosaccharide On Cecal Salmonella Enteritidis Colonization And Intestinal Parameters Of Broilers, Sucheera Chotikatum, Indhira Kramomthong, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

Medium chain fatty acids (MCA) are one of new additives used to control Salmonella infection and it is interesting to examine their physiological role in chickens comparing with other additives. The objective of this investigation was to study the effect of MCA, mixed organic acids (ORA) and fructo-oligosaccharide (FOS) on cecal Salmonella enterica serovar Enteritidis colonization and physiological changes of intestine in broilers. Six hundred, day old, male and female broiler chicks were allocated into 4 treatments. The treatments were CON: basal corn-soybean meal diet, FOS: basal diet supplemented with 4 g/kg FOS, ORA: basal diet plus water supplemented with …


Penaeid Shrimp Immune System, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Angel Isidro Campa-Cordova, Antonio Luna-Gonzalez, Felipe Ascencio Sep 2009

Penaeid Shrimp Immune System, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Angel Isidro Campa-Cordova, Antonio Luna-Gonzalez, Felipe Ascencio

The Thai Journal of Veterinary Medicine

Research on an innate immune system of penaeid shrimp is greatly motivated by economical requirements, because their culture is limited by the development of infectious diseases. As invertebrates, shrimp’s natural immunity acts as a fast and efficient defence mechanism against the pathogens. Their immune system involve hemocytes (for encapsulation, nodule formation and phagocytosis), several plasma components (antimicrobial peptides, histones, lysosomal enzymes, lipopolysaccharide, β-1,3-glucan binding proteins, and recognition molecules), and multimeric systems (clotting protein cascade, prophenoloxidase system). When these defense mechanisms fail to protect the shrimp against bacteria, viruses, fungi, protozoa and their products, disease develops and a negative impact takes …


Comparative Efficacy Of Enrofloxacin And Oxytetracycline By Different Administration Methods In Broilers After Experimental Infection With Avian Pathogenic Escherichia Coli, Niwat Chansiripornchai Sep 2009

Comparative Efficacy Of Enrofloxacin And Oxytetracycline By Different Administration Methods In Broilers After Experimental Infection With Avian Pathogenic Escherichia Coli, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The aims of this study were to compare the efficacy of 2 kinds of antibiotics that are commonly used in the Thai broiler industry and to determine the optimal times of drug administration. One hundred and sixty, 21-day-old broilers were divided into eight groups. Chickens in groups 1, 2 and 3 were challenged with avian pathogenic E. coli (APEC) and received oxytetracycline for the first 3 days after infection via oral, drinking water within 2 h and drinking water throughout the day, respectively. The chickens in groups 4, 5 and 6 were challenged with APEC and received enrofloxacin for the …


Efficacy Of Microsatellite Markers In Parentage Control In Swine, Wanwisa Yaemmeeklin, Jutarat Jirasupphachok, Weerapong Koykul, Duangsmorn Suwattana Sep 2009

Efficacy Of Microsatellite Markers In Parentage Control In Swine, Wanwisa Yaemmeeklin, Jutarat Jirasupphachok, Weerapong Koykul, Duangsmorn Suwattana

The Thai Journal of Veterinary Medicine

The efficacy of microsatellite markers was evaluated in order to use in parentage control in swine. Genomic DNA from 80 samples were extracted, and amplified using 16 microsatellite markers (D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, S0766, SJ859, SJ923, SJ924, SJ925, SJ926, SJ927, SJ929, X53085 and X63893) in each single polymerase chain reaction (PCR). The PCR products were analyzed using agarose and polyacrylamide gel electrophoresis (PAGE). The result showed that 15 microsatellite loci could be amplified except SJ925. Seven suitable microsatellite markers were selected for parentage control, including D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, and S0766. Allelic numbers of the selected …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn Sep 2009

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Sep 2009

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai Sep 2009

Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai

The Thai Journal of Veterinary Medicine

Mastitis is a most frequent and costly disease of dairy cattle worldwide. All three contagious mastitis pathogens, Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were investigated in 55 bulk tank milk samples from dairy cattle herds in Khon Kaen Province Thailand, by nested PCR and/or conventional bacterial culture. Bulk milk somatic cell count (BMSCC) was used as indicator for mastitis problem; i.e. > 5x105 somatic cells/ml of milk. The prevalence of Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were 1.8%, 21.8% and 7.3%, respectively. Results from BMSCC indicated mastitis problem in 47 herds. However, 34 high-BMSCC with negative-contagious pathogen identification samples …


Successful Transcervical Insemination In Giant Panda At Chiangmai Zoo, Thailand, Boripat Siriaroonrat, Ampika Thongphakdee, Wanlaya Tipkantha, Kannikar Nimtragul, Kornchai Kornkeawrat, Ratchaneewan Khejornwong, Prasertsak Buntragulpoontawee, Sumate Kamolnorranath Sep 2009

Successful Transcervical Insemination In Giant Panda At Chiangmai Zoo, Thailand, Boripat Siriaroonrat, Ampika Thongphakdee, Wanlaya Tipkantha, Kannikar Nimtragul, Kornchai Kornkeawrat, Ratchaneewan Khejornwong, Prasertsak Buntragulpoontawee, Sumate Kamolnorranath

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep Sep 2009

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa Sep 2009

Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa

The Thai Journal of Veterinary Medicine

This study was to conduct a surveillance of Mycoplasma synoviae (MS) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1-4.5 months in the area of Nakornpathom province in the September 2005-October 2006 period. Each bird was bled for MS serology by serum plate agglutination (SPA) and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MS antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA and PCR procedure were 12, 9 and 8 flocks, …


Proliferation And Apoptosis Of The Bitch Ovary During The Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Sukanya Manee-In, Promporn Raksaseri, Kongkiat Srisuwatanasagul Sep 2009

Proliferation And Apoptosis Of The Bitch Ovary During The Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Sukanya Manee-In, Promporn Raksaseri, Kongkiat Srisuwatanasagul

The Thai Journal of Veterinary Medicine

In order to maintain homeostasis of the ovary, proliferation and apoptosis occur simultaneously in different ovarian cell types. It was shown that steroid hormone which changed during the oestrous cycle, may regulate these mechanisms in normal mammal ovarian tissues. Therefore, the present study was aimed to study cell proliferation and apoptosis during different stages of the oestrous cycle in the bitch ovary by using immunohistochemistry and TUNEL assay. The bitch ovaries were collected at 4 different stages of the oestrous cycle, which were prooestrus, oestrus, dioestrus and anoestrus. Immunohistochemistry and TUNEL assay were applied to detect Ki-67 protein and apoptosis …


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep Jun 2009

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Comparison Of The Efficacy Of The Immune Complex And Conventionally Live Vaccine In Broilers Against Infectious Bursal Disease Infection, Niwat Chansiripornchai, Jiroj Sasipreeyajan Jun 2009

Comparison Of The Efficacy Of The Immune Complex And Conventionally Live Vaccine In Broilers Against Infectious Bursal Disease Infection, Niwat Chansiripornchai, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

One hundred and thirty two, one day old broiler chickens were divided into 4 groups. Group 1 (1 day old) was subcutaneously vaccinated with immune complex vaccine into the nape of the neck. Group 2 and 3 (14 days old) were vaccinated with different commercial products, intermediate-plus strains of live attenuated infectious bursal disease (IBD) vaccine I and II, respectively, via the oral route. Group 4 acted as the positive control. Chickens were bled at days 1, 14, 28 and 38 for evaluating IBD virus antibody titers by ELISA. The body weight, FCR, bursa to body weight ratios and bursal …