Medicine and Health Sciences Commons^™

Autoregulation Of Cortical Blood Flow In Response To Phenylephrine Induced Hypertension In The Normocapnic And Hypercapnic Cat, Forrest Ritland

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this research was to determine the degree of autoregulation of cortical blood flow in the cat, and to evaluate the usefulness of the heated thermocouple technique for measurement of local cortical blood flow. We determined the relationship between local flow and voltage output from our thermoelectric flow probe by in vitro experiments with an artificial kidney. We measured local cortical blood flow in 17 cats during episodes of phenylephrine-induced hypertension, under conditions of normocapnia and, in some cases, hypercapnia. Arterial blood pressure and voltage output from the flow probe thermocouples were recorded continuously with a Physiograph-six monitor. …

The Effects Of Insulin On Hepatic Glucocorticoid Receptor Content In The Diabetic Rat, Jeffrey John Yourick

Masters Theses

The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.

The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. …

The Effects Of Prostaglandin E2 On The Healing Processes Of Rat Perforated Tympanic Membranes, Diane Sue Saylor

Masters Theses

Prostaglandin E₂ (PGE₂) was topically administered to perforated rat tympanic membranes in an attempt to facilitate regrowth of the membranes. Every 24 hours for the first four postperforation days, two groups of tympanic membranes were processed and examined histologically for differences between PGE₂ treated and control animals.

At 24 hours postperforation granulocyte infiltration at the perforation site was greater in the test animals. By 48 hours blood clots were larger in PGE₂ treated animals. Finally, the epithelium was thinner in test animals than in control animals at 96 hours.

Slight tendencies for the test animals …