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Full-Text Articles in Medicine and Health Sciences

Quarantine And Health Screening Protocols For Wildlife Prior To Translocation And Release Into The Wild, Michael H. Woodford Dec 2000

Quarantine And Health Screening Protocols For Wildlife Prior To Translocation And Release Into The Wild, Michael H. Woodford

Other Publications in Zoonotics and Wildlife Disease

In recent years the translocation and release into the wild of wild-caught and captive-bred wild animals (mammals, birds, reptiles, amphibians and fish) has become a common practice, ostensibly for rehabilitation or conservation purposes. These wild animals comprise many varied taxa and the objectives of translocation and release may include:
(1) reintroducing a species that has become extinct in its natural range;
(2) restocking or reinforcing a population which has become depleted; and
(3) rehabilitating wild animals and birds which have been illegally captured and subsequently confiscated by Customs or national wildlife authorities. Welfare organizations also receive sick and injured wild …


Amino Acid Osmolytes In Regulatory Volume Decrease And Isovolumetric Regulation In Brain Cells: Contribution And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, M. Eugenia Torres-Marquez, Karla Hernandez-Fonseca, Arturo Ortega Oct 2000

Amino Acid Osmolytes In Regulatory Volume Decrease And Isovolumetric Regulation In Brain Cells: Contribution And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, M. Eugenia Torres-Marquez, Karla Hernandez-Fonseca, Arturo Ortega

School of Veterinary and Biomedical Sciences: Faculty Publications

Brain adaptation to hyposmolarity is accomplished by loss of both electrolytes and organic osmolytes, including amino acids, polyalcohols and methylamines. In brain in vivo, the organic osmolytes account for about 35% of the total solute loss. This review focus on the role of amino acids in cell volume regulation, in conditions of sudden hyposmosis, when cells respond by active regulatory volume decrease (RVD) or after gradual exposure to hyposmotic solutions, a condition where cell volume remains unchanged, named isovolumetric regulation (IVR). The amino acid efflux pathway during RVD is passive and is similar in many respects to the volume-activated anion …


Clinical, Pathological And Antigenic Aspects Of Bovine Viral Diarrhea Virus (Bvdv) Type 2 Isolates Identified In Brazil, E. F. Flores, L. H. G. V. Gil, S. A. Botton, R. Weiblen, J. F. Ridpath, L. C. Kreutz, C. Pilati, D. Driemeyer, V. Moojen, A. C. Wendelstein Oct 2000

Clinical, Pathological And Antigenic Aspects Of Bovine Viral Diarrhea Virus (Bvdv) Type 2 Isolates Identified In Brazil, E. F. Flores, L. H. G. V. Gil, S. A. Botton, R. Weiblen, J. F. Ridpath, L. C. Kreutz, C. Pilati, D. Driemeyer, V. Moojen, A. C. Wendelstein

School of Veterinary and Biomedical Sciences: Faculty Publications

Nucleotide sequencing and phylogenetic analysis of Brazilian bovine viral diarrhea virus (BVDV) field isolates identified four viruses belonging to the genotype 2. Comparison of 5’ UTR sequences from these isolates to those of North American BVDV type 2 revealed genomic variations that correlated with the geographic origins of the isolates. Two of the Brazilian type 2 viruses were isolated from clinical cases of gastroenteric/respiratory disease and two were isolated from healthy bovine fetuses. The clinical cases affected young animals (8- and 18-months-old) and were characterized by diarrhea, respiratory signs, extensive oral and digestive tract erosions, conjunctival and vulvar congestion, occasional …


Relative Volume Of The Cerebellum In Dolphins And Comparison With Anthropoid Primates, L. Marino, James K. Rilling, Shinko K. Lin, Sam H. Ridgway Oct 2000

Relative Volume Of The Cerebellum In Dolphins And Comparison With Anthropoid Primates, L. Marino, James K. Rilling, Shinko K. Lin, Sam H. Ridgway

Veterinary Science and Medicine Collection

According to the ‘developmental constraint hypothesis’ of comparative mammalian neuroanatomy, brain growth follows predictable allometric trends. Therefore, brain structures should scale to the entire brain in the same way across mammals. Evidence for a departure from this pattern for cerebellum volume has recently been reported among the anthropoid primates. One of the mammalian groups that has been neglected in tests of the ‘developmental constraint hypothesis’ is the cetaceans (dolphins, whales, and porpoises). Because many cetaceans possess relative brain sizes in the range of primates comparative tests of the ‘developmental constraint hypothesis’ across these two groups could help to delineate the …


Hsv Latency-Associated Transcript And Neuronal Apoptosis, Anthony B. Nesburn, Clinton J. Jones Sep 2000

Hsv Latency-Associated Transcript And Neuronal Apoptosis, Anthony B. Nesburn, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Thompson and Sawtell report that the Promega Anti-PARP p85 antibody did not recognize cleaved PARP in mouse or rabbit cells in their experiments, and conclude that the results reported with this antibody by Perng et al. (1) are an artifact. The Promega antibody was generated against a peptide based on the sequence of human p85. Although the corresponding bovine sequence differs by two amino acids, the antibody reacts with both human and bovine p85 (2). The mouse and rat sequences for this region of p85 differ from the human sequence by a single amino acid that corresponds to one of …


Intestinal Lesions Caused By A Strain Of Chlamydia Suis In Weanling Pigs Infected At 21 Days Of Age, Douglas G. Rogers, Arthur A. Andersen Sep 2000

Intestinal Lesions Caused By A Strain Of Chlamydia Suis In Weanling Pigs Infected At 21 Days Of Age, Douglas G. Rogers, Arthur A. Andersen

School of Veterinary and Biomedical Sciences: Faculty Publications

The objective of this study was to determine whether a strain of Chlamydia suis shown previously to be an intestinal pathogen in gnotobiotic piglets could cause diarrhea and intestinal lesions in young weanling pigs. Pigs from 2 sows were randomly assigned to 2 groups. Group 1 included 13 pigs that were weaned at 24 hours of age and then housed in isolator units and fed milk replacer and unmedicated starter ration. Group 2 included 8 pigs that nursed their respective sows, consumed unmedicated starter ration, and were weaned at 21 days of age. Ten pigs in group 1 and 6 …


Sarcocystis Neurona Diagnostic Primer And Its Use In Methods Of Equine Protozoal Myeloencephalitis Diagnosis, Clara K. Fenger, David E. Granstrom, Alvin A. Gajadhar, Jitender P. Dubey Aug 2000

Sarcocystis Neurona Diagnostic Primer And Its Use In Methods Of Equine Protozoal Myeloencephalitis Diagnosis, Clara K. Fenger, David E. Granstrom, Alvin A. Gajadhar, Jitender P. Dubey

Veterinary Science Faculty Patents

An amplification primer and probe which can be used in an in vitro diagnostic test for the presence of S. neurona in equine blood or cerebrospinal fluid. Sarcocystis neurona is responsible for the equine condition of protozoal myelitis. The amplification primer is seventeen nucleotides in length and complementary to a unique section of the small ribosomal subunit of Sarcocystis neurona. The primer encompasses nucleotide positions 1470-1487 of the small ribosomal subunit of S. neurona. The primer has the sequence 5' CCATTCCGGACGCGGGT SEQ ID NO:1.


Naturally Occurring Tuberculosis In White-Tailed Deer, Mitchell V. Palmer, Diana L. Whipple, Janet B. Payeur, David P. Alt, Kevin J. Esch, Colleen S. Bruning-Fann, John B. Kaneene Jun 2000

Naturally Occurring Tuberculosis In White-Tailed Deer, Mitchell V. Palmer, Diana L. Whipple, Janet B. Payeur, David P. Alt, Kevin J. Esch, Colleen S. Bruning-Fann, John B. Kaneene

Michigan Bovine Tuberculosis Bibliography and Database

Objective—To determine the distribution of lesions and extent of tissues infected with Mycobacterium bovis in a captive population of white-tailed deer.

Design—Cross-sectional study.

Animals—116 captive white-tailed deer.

Procedure—Deer were euthanatized, and postmortem examinations were performed. Tissues with gross lesions suggestive of tuberculosis were collected for microscopic analysis and bacteriologic culture. Tissues from the head, thorax, and abdomen of deer with no gross lesions were pooled for bacteriologic culture. Tonsillar, nasal, oral, and rectal swab specimens, fecal samples, and samples of hay and pelleted feed, soil around feeding sites, and water from 2 natural ponds were collected …


Efflux Of Osmolyte Amino Acids During Isovolumic Regulation In Hippocampal Slices, Rodrigo Franco, Octavio Quesada, Herminia Pasantes-Morales May 2000

Efflux Of Osmolyte Amino Acids During Isovolumic Regulation In Hippocampal Slices, Rodrigo Franco, Octavio Quesada, Herminia Pasantes-Morales

School of Veterinary and Biomedical Sciences: Faculty Publications

The efflux of potassium (K+) and amino acids from hippocampal slices was measured after sudden exposure to 10% (270 mOsm), 25% (225 mOsm) or 50% (150 mOsm) hyposmotic solutions or after gradual decrease (22.5 mOsm/min) in external osmolarity. In slices suddenly exposed to 50% hyposmotic solutions, swelling was followed by partial (74%) cell volume recovery, suggesting regulatory volume decrease (RVD). With gradual hyposmotic changes, no increase in cell water content was observed even when the solution at the end of the experiment was 50% hyposmotic, showing the occurrence of isovolumic regulation (IVR). The gradual decrease in osmolarity elicited …


Ceftriaxone-Resistant Salmonella Infection Acquired By A Child From Cattle, Paul D. Fey, Thomas J. Safranek, Mark E. Rupp, Eileen F. Dunne, Efrain Ribot, Peter C. Iwen, Patricia A. Bradford, Frederick J. Angulo, Steven H. Hinrichs Apr 2000

Ceftriaxone-Resistant Salmonella Infection Acquired By A Child From Cattle, Paul D. Fey, Thomas J. Safranek, Mark E. Rupp, Eileen F. Dunne, Efrain Ribot, Peter C. Iwen, Patricia A. Bradford, Frederick J. Angulo, Steven H. Hinrichs

Other Publications in Zoonotics and Wildlife Disease

Background
The emergence of resistance to antimicrobial agents within the salmonellae is a worldwide problem that has been associated with the use of antibiotics in livestock. Resistance to ceftriaxone and the fluoroquinolones, which are used to treat invasive salmonella infections, is rare in the United States. We analyzed the molecular characteristics of a ceftriaxone-resistant strain of Salmonella enterica serotype typhimurium isolated from a 12-year-old boy with fever, abdominal pain, and diarrhea.
Methods
We used pulsed-field gel electrophoresis and analysis of plasmids and β-lactamases to compare the ceftriaxone-resistant S. enterica serotype typhimurium from the child with four isolates of this strain …


Genetic Resistance To Experimental Infection With Mycobacterium Bovis In Red Deer (Cervus Elaphus), Colin G. Mackintosh, Tariq Qureshi, Ken Waldrup, Robert E. Labes, Ken G. Dodds, J. Frank T. Griffin Mar 2000

Genetic Resistance To Experimental Infection With Mycobacterium Bovis In Red Deer (Cervus Elaphus), Colin G. Mackintosh, Tariq Qureshi, Ken Waldrup, Robert E. Labes, Ken G. Dodds, J. Frank T. Griffin

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis (Tb) caused by Mycobacterium bovis is a worldwide threat to livestock and humans. One control strategy is to breed livestock that are more resistant to Mycobacterium bovis. In a 3-year heritability study 6 farmed red deer stags were selected from 39 on the basis of their differing responses to experimental challenge via the tonsillar sac with approximately 500 CFU of M. bovis. Two stags remained uninfected, two were moderately affected, and two developed serious spreading Tb. Seventy offspring, bred from these six stags by artificial insemination using stored semen, were similarly challenged with M. bovis. The …


Proceedings From The Conference On Bovine Tuberculosis – March 2000 Mar 2000

Proceedings From The Conference On Bovine Tuberculosis – March 2000

Michigan Bovine Tuberculosis Bibliography and Database

Table of Contents:

The Challenge, USDA by Craig Reed, DVM; John Clifford, DVM

The Comprehensive Strategic Plan by Joe VanTiem, DVM, MS

Surveillance of Non-Cervid (Cattle and Goats) Livestock by Nathan Zauel, DVM

Surveillance of Bovine Tuberculosis in Michigan Captive Cervidae Mike VanderKlok, DVM Surveillance of Domestic Carnivores for Tuberculosis Steven L. Halstead, DVM, MS Human Health Update: Bovine Tuberculosis in Michigan by Barbara Robinson-Dunn, PhD; Mary Grace Stobierski, DVM, MPH

Risks Associated with M. bovis in Michigan Free-ranging White-tailed Deer by Barbara Corso, DVM, MS

Economic Impact of Wildlife-Related Recreation in the 5-County Area: An Update by Dennis Propst, …


Virus-Induced Neuronal Apoptosis Blocked By The Herpes Simplex Virus Latency-Associated Transcript, Guey-Chuen Perng, Clinton J. Jones, Janice Ciacci-Zanella, Melissa Stone, Gail A. Henderson, Ada Yukht, Susan M. Slanina, Florence M. Hofman, Homayon Ghiasi, Anthony B. Nesburn, Steven L. Wechsler Feb 2000

Virus-Induced Neuronal Apoptosis Blocked By The Herpes Simplex Virus Latency-Associated Transcript, Guey-Chuen Perng, Clinton J. Jones, Janice Ciacci-Zanella, Melissa Stone, Gail A. Henderson, Ada Yukht, Susan M. Slanina, Florence M. Hofman, Homayon Ghiasi, Anthony B. Nesburn, Steven L. Wechsler

School of Veterinary and Biomedical Sciences: Faculty Publications

Latent infections with periodic reactivation are a common outcome after acute infection with many viruses. The latency-associated transcript (LAT) gene is required for wild-type reactivation of herpes simplex virus (HSV). However, the underlying mechanisms remain unclear. In rabbit trigeminal ganglia, extensive apoptosis occurred with LAT- virus but not with LAT+ viruses. In addition, a plasmid expressing LAT blocked apoptosis in cultured cells. Thus, LAT promotes neuronal survival after HSV-1 infection by reducing apoptosis.


Agonistic Behaviour And Biogenic Amines In Shore Crabs Carcinus Maenas, Lynne U. Sneddon, Alan C. Taylor, Felicity A. Huntingford, David G. Watson Feb 2000

Agonistic Behaviour And Biogenic Amines In Shore Crabs Carcinus Maenas, Lynne U. Sneddon, Alan C. Taylor, Felicity A. Huntingford, David G. Watson

Sentience Collection

To investigate the role of certain neurohormones in agonistic behaviour, fights were staged between pairs of size-matched male shore crabs Carcinus maenas, and blood samples were taken immediately after the contests had been resolved. Samples were also taken from these crabs at rest (before and after fighting) and after walking on a treadmill. A control group of crabs also had samples taken on each experimental day. Concentrations of tyramine, dopamine, octopamine, serotonin (5-HT) and norepinephrine were determined in each blood sample using a gas chromatography/mass spectrometry (GC-MS) system. Norepinephrine was not detectable in any of the samples, but the standards …


Department Of Veterinary And Biomedical Sciences: 2000 Annual Report Jan 2000

Department Of Veterinary And Biomedical Sciences: 2000 Annual Report

Veterinary and Biomedical Sciences -- Department Information and History

Foreword, 2000 John A . Schmitz. Professor and Department Head
Veterinary and Biomedical Sciences Personnel
Faculty Profiles
Teaching program
Research program
Extension Program
Nebraska Veterinary and Diagnostic Laboratory Systems
Grants and Contracts Funded or Active in 2000
Patents by VBMS Faculty in 2000
Publications by VBMS Faculty in 2000
Presentations by VBMS Faculty in 2000
Articles Regarding the Department in 2000
Selected Committees, Editorial and Other Appointments of VBMS Faculty
Departmental Budget Summaries
Nebraska Agricultural Statistics 1999


Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz Jan 2000

Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

Cell volume regulation is a property present in most animal cell lineages that allows them to recover their original volume after events of swelling or shrinkage. Such events can be caused by changes in external osmolarity or to osmotic gradients generated during normal cell functioning.4,6 The mechanism of cell volume regulation involves transmembrane fluxes of osmotically active solutes in the necessary direction to counteract the net gain or loss of intracellular water.9 The process through which cells recover their normal volume after swelling is named Regulatory Volume Decrease (RVD). This consists of the efflux of inorganic osmolytes, such as K …


Effect Of Amitraz And Xylazine On Some Physiological Variables Of Horses, A. Queiroz-Neto, A. B. Carregaro, G. Zamur, J Daniel Harkins, Thomas Tobin, M. I. Mataqueiro, S. C. Gonçalves Jan 2000

Effect Of Amitraz And Xylazine On Some Physiological Variables Of Horses, A. Queiroz-Neto, A. B. Carregaro, G. Zamur, J Daniel Harkins, Thomas Tobin, M. I. Mataqueiro, S. C. Gonçalves

Maxwell H. Gluck Equine Research Center Faculty Publications

The effect of the intravenous injection of amitraz (0.1 mg/kg) to horses on cardiac activity, respiratory rate, intestinal motor activity, rectal temperature, sweating and masticating was evaluated and compared to the effect of intravenous administration of xylazine (1mg/kg, iv) on heart rate, respiratory rate and intestinal activity. Amitraz caused a significant decrease in heart activity, respiratory rate and intestinal movements, but these effects were not as pronounced as those caused by xylazine. Amitraz also caused a significant relaxation of the rectal smooth muscle, and an apparent increase in sweating and in the frequency of horses found masticating hay. Rectal temperature …


Remifentanil In The Horse: Identification And Detection Of Its Major Urinary Metabolite, A. F. Lehner, P. Almeida, J. Jacobs, J Daniel Harkins, W. Karpiesiuk, W. E. Woods, L. Dirikolu, Jeffrey M Bosken, W. G. Carter, J. Boyles, C. Holtz, T. Heller, C. Nattrass, M. Fisher, Thomas Tobin Jan 2000

Remifentanil In The Horse: Identification And Detection Of Its Major Urinary Metabolite, A. F. Lehner, P. Almeida, J. Jacobs, J Daniel Harkins, W. Karpiesiuk, W. E. Woods, L. Dirikolu, Jeffrey M Bosken, W. G. Carter, J. Boyles, C. Holtz, T. Heller, C. Nattrass, M. Fisher, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Remifentanil (4-methoxycarbonyl-4-[(1-oxopropyl)phyenylamino]-1- piperidinepropionic acid methyl ester) is a μ-opioid receptor agonist with considerable abuse potential in racing horses. The identification of its major equine urinary metabolite, 4-methoxycarbonyl-4-[(1- oxopropyl)phenylamino]-1-piperidinepropionic acid, an ester hydrolysis product of remifentanil is reported. Administration of remifentanil HCl (5 mg, intravenous) produced clear-cut locomotor responses, establishing the clinical efficacy of this dose. ELISA analysis of postadministration urine samples readily detected fentanyl equivalents in these samples. Mass spectrometric analysis, using solid-phase extraction and trimethylsilyl (TMS) derivatization, showed the urine samples contained parent remifentanil in low concentrations, peaking at 1 h. More significantly, a major peak was identified as …


Comparison Of Postmortem Techniques For The Detection Of Mycobacterium Bovis In White-Tailed Deer (Odocoileus Virginianus), Scott D. Fitzgerald, John B. Kaneene, Kelly L. Butler, Kathy R. Clarke, Jean S. Fierke, Stephen M. Schmitt, Colleen S. Bruning-Fann, Rachel R. Mitchell, Dale E. Berry, Janet B. Payeur Jan 2000

Comparison Of Postmortem Techniques For The Detection Of Mycobacterium Bovis In White-Tailed Deer (Odocoileus Virginianus), Scott D. Fitzgerald, John B. Kaneene, Kelly L. Butler, Kathy R. Clarke, Jean S. Fierke, Stephen M. Schmitt, Colleen S. Bruning-Fann, Rachel R. Mitchell, Dale E. Berry, Janet B. Payeur

Michigan Bovine Tuberculosis Bibliography and Database

A retrospective study of various diagnostic postmortem techniques used in a 4-year surveillance program for detection of Mycobacterium bovis infection in wild white-tailed deer (Odocoileus virginianus) was conducted. The tests evaluated were routine histopathology, acid-fast staining, detection of acid-fast bacilli in culture, and an M. tuberculosis group-specific genetic probe applied to pure cultures. Each of these techniques were compared with a reference or “gold standard” of mycobacterial culture and identification. Histopathology, the most rapid form of testing for M. bovis infection in white-tailed deer samples, had a sensitivity of 98% and a specificity of 87%, resulting in a …


Use Of Gis To Evaluate Livestock-Wildlife Interactions Relative To Tuberculosis Spread On Molokai Island, Hawaii, Stephanie Bany, Jerome Freier Jan 2000

Use Of Gis To Evaluate Livestock-Wildlife Interactions Relative To Tuberculosis Spread On Molokai Island, Hawaii, Stephanie Bany, Jerome Freier

Michigan Bovine Tuberculosis Bibliography and Database

Historically, bovine tuberculosis has occurred and been eradicated several times on the Hawaiian island of Molokai. After the most recent depopulation of cattle, TB was again identified in an adult cow in 1997, thus continuing the investigation into why bovine tuberculosis is reoccurring on the island. The Centers for Epidemiology and Animal Health (CEAH) have implemented spatial analysis using ArcView software that, along with epidemiological investigations, attempts to identify the environmental factors responsible for maintaining this disease in natural foci. To examine the ecological aspects of tuberculosis transmission from wildlife to livestock, and to identify the foci of infection, we …


Elaeophorosis In Red Deer From Spain, Mónica Santín-Durán, J. M. Alunda, J. M. San Miguel, Eric P. Hoberg, C. De La Fuente Jan 2000

Elaeophorosis In Red Deer From Spain, Mónica Santín-Durán, J. M. Alunda, J. M. San Miguel, Eric P. Hoberg, C. De La Fuente

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Elaeophorosis, caused by Elaeophora elaphi, was observed in red deer (Cervus elaphus) from Toledo Province (Spain) for the first time. Adult specimens of Elaeophora elaphi were found in the hepatic vessels of nine of 151 red deer between October 1994 and September 1995; intensity of infection was two to 18 nematodes per host. Adult nematodes were only found during the period from fall through early spring. No differences were present between sex or age groups. Parasites were not found in a limited sample from fallow deer (Dama dama). Blood samples were negative for the presence of …


Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase Jan 2000

Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase

Qingsheng Li Publications

Mycobacterium avium is the most frequent cause of disseminated bacterial infection in patients with human immunodeficiency virus type 1 infection and in rhesus macaques with simian immunodeficiency virus (SIV) infection. This animal model of AIDS was used to test the hypothesis that this frequent association is the result of reciprocal enhancement of replication of both microorganisms. The replication of M. avium and SIV was analyzed in lymphatic tissues obtained from rhesus macaques experimentally inoculated with SIVmac who developed or remained free of overt M. avium infection. In situ hybridization, quantitative image analysis, and staining of M. avium and of macrophages …


Mortality In Serologically Unconfirmed Mediterranean Spotted Fever [With Reply], Pablo Yagupsky, Christopher D. Paddock, James E. Childs, Sherif R. Zaki, Stephen A. Berger Jan 2000

Mortality In Serologically Unconfirmed Mediterranean Spotted Fever [With Reply], Pablo Yagupsky, Christopher D. Paddock, James E. Childs, Sherif R. Zaki, Stephen A. Berger

Other Publications in Zoonotics and Wildlife Disease

I read with interest the article by Paddock et al. [1], who described the detection of occult mortality due to Rocky Mountain spotted fever (RMSF) by demonstrating Rickettsia rickettsii antigens or DNA in blood and tissues. In Mediterranean countries, including Israel, spotted fever is caused by members of the R. conorii complex, which are antigenically related to R. rickettsii. ...

To the Editor -- We appreciate the comments by Yagupsky, which underscore several of the salient features of spotted fever- group rickettsial infections that we emphasized in our report [2]. For patients, diagnosticians, and clinicians faced with the potentially tragic …


Prevalence Of Antibody To Hepatitis E Virus Among Rodents In The United States, Michael O. Favorov, Michael Y. Kosoy, Sergei A. Tsarev, James E. Childs, Harold S. Margolis Jan 2000

Prevalence Of Antibody To Hepatitis E Virus Among Rodents In The United States, Michael O. Favorov, Michael Y. Kosoy, Sergei A. Tsarev, James E. Childs, Harold S. Margolis

Other Publications in Zoonotics and Wildlife Disease

The recent identification of antibody to hepatitis E virus (HEV) in pigs, sheep, and cattle and characterization of an HEV isolated from domestic pigs suggest animal reservoirs for this virus. To investigate whether rodents might be a natural reservoir of HEV, the prevalence of anti-HEV was determined among a variety of species throughout the United States. Serum samples were obtained from 806 rodents of 26 species in 15 genera. Anti-HEV prevalence was assessed by 2 EIAs (mosaic protein- and 55-kDa protein-based), which gave concordant results. The highest prevalence of antibody was found in the genus Rattus (59.7%; 66/278). Overall, rodents …


Primary Isolation Of Ehrlichia Chaffeensis From Patients With Febrile Illnesses: Clinical And Molecular Characteristics, Steven M. Standaert, Tina Yu, Margie A. Scott, James E. Childs, Christopher D. Paddock, William L. Nicholson, Joseph Singleton Jr., Martin J. Blaser Jan 2000

Primary Isolation Of Ehrlichia Chaffeensis From Patients With Febrile Illnesses: Clinical And Molecular Characteristics, Steven M. Standaert, Tina Yu, Margie A. Scott, James E. Childs, Christopher D. Paddock, William L. Nicholson, Joseph Singleton Jr., Martin J. Blaser

Other Publications in Zoonotics and Wildlife Disease

Ehrlichia chaffeensis was sought among patients with a history of tick exposure and fever, and the accuracy of other diagnostic tests was compared with that of primary isolation. Among the 38 patients enrolled, E. chaffeensis was isolated from the blood of 7 (18%) and from cerebrospinal fluid specimens of 2 of these 7. All 7 patients also were positive by polymerase chain reaction (PCR) of blood, and 6 patients developed diagnostic titers of antibody to E. chaffeensis. The isolates were characterized by molecular analysis of the 16S rRNA gene, the 120-kDa protein gene, and the variable-length PCR target (VLPT) …


Predicting The Local Dynamics Of Epizootic Rabies Among Raccoons In The United States, Centers For Disease Control And Prevention, Emory University, National Center For Ecological Analysis And Synthesis Jan 2000

Predicting The Local Dynamics Of Epizootic Rabies Among Raccoons In The United States, Centers For Disease Control And Prevention, Emory University, National Center For Ecological Analysis And Synthesis

Other Publications in Zoonotics and Wildlife Disease

Mathematical models have been developed to explore the population dynamics of viral diseases among wildlife. However, assessing the predictions stemming from these models with wildlife databases adequate in size and temporal duration is uncommon. An epizootic of raccoon rabies that began in the mid-Atlantic region of the United States in the late 1970s has developed into one of the largest and most extensive in the history of wildlife rabies. We analyzed the dynamics of local epizootics at the county level by examining a database spanning more than 20 years and including 35,387 rabid raccoons. The size, number, and periodicity of …


Incidence Of Rocky Mountain Spotted Fever Among American Indians In Oklahoma, Jennifer H. Mcquiston, Robert C. Holman, Amy V. Groom, Stephen F. Kaufman, James E. Cheek, James E. Childs Jan 2000

Incidence Of Rocky Mountain Spotted Fever Among American Indians In Oklahoma, Jennifer H. Mcquiston, Robert C. Holman, Amy V. Groom, Stephen F. Kaufman, James E. Cheek, James E. Childs

Other Publications in Zoonotics and Wildlife Disease

Althought he state of Oklahoma has traditionally reported very high incidence rates of Rocky Mountain spotted fever (RMSF) cases, the incidence of RMSF among the American Indian population of the state has not been studied. The authors used data from several sources to estimate the incidence of RMSF among American Indians in Oklahoma.